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Abstract

In this study, the effect of sildenafil citrate on some haematological parameters and mineral levels in the early stages of wound healing was investigated.Rats were randomly allocated into three treatment groups consisted of 10 rats in each. Groups were designed as control (group I), diabetic (group II) and diabetic plus sildenafil citrate (group III), respectively. Normal wound care was performed for control and group II. Sildenafil citrate was also administered with normal wound care for group III at 0.7 mg/kg dosage for 3 days via intraperitoneally (i.p.) once a day. Blood samples were collected before and at 1., 2., and., 3. days of after administration. Full blood erythrocyte (RBC), haemoglobin (HG), haematocrit (HCT) values were determined using standard methods; spectrophotometric methods were used for determination of the serum iron (Fe), manganese (Mn), copper (Cu), zinc (Zn), lead (Pb), potassium (K), calcium (Ca), and magnesium (Mg) levels. Sildenafil citrate increased the serum mineral levels (i.e. Zn, Cu and Mg), as having favourable impact on diabetic wound healing process. Likewise, it decreased the level of iron mineral, as having an adverse effect on the healing process. In conclusion, it was concluded that sildenafil citrate had a favourable effect on the healing process by shortening the inflammatory period, which is the first period of wound healing process

Keywords

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Deneysel Olarak Diabet Oluşturulmuş Ratlarda Yara İyileşmesinde Sildenafil Sitratın Bazı Hematolojik Parametrelere ve Mineral Maddelere Etkisi

Öz

Bu çalışmada sildenafil sitratın yara iyileşmesinin erken dönemlerinde bazı hematolojik parametreler ve mineral maddeler üzerine olan etkisi araştırıldı. Bu amaçla her grupta 10’ar adet rat olacak şekilde üç grup (1. Grup: Kontrol, 2. Grup: Diabet ve 3. Grup: Diabet + Sildenafil Sitrat) oluşturuldu. Birinci ve ikinci gruba normal yara bakımı, üçüncü gruba ise normal yara bakımının yanında 0.7 mg/kg dozunda 3 gün süreyle günde bir kez olmak üzere intraperitonal (i.p.) sildenafil sitrat verildi. Uygulamalara başlamadan önce 1. 2. ve 3. günlerde kan alındı. Tam kanda eritrosit (RBC), hemoglobin (HG), hematokrit (HCT), değerlerine klasik yöntemlerle; serumda demir(Fe), mangan (Mn), bakır (Cu), çinko (Zn), kurşun (Pb), potasyum (K), kalsiyum (Ca) ve magnezyum (Mg) değerlerine ise spektrofotometrik metodla bakıldı. Sildenafil sitrat, diabetli yaralarda iyileşme sürecine olumlu etki eden mineralllerden Zn, Cu ve Mg değerlerini arttırdı. İlk dönemlerde iyileşme sürecine olumsuz etki eden Fe minerali değerlerini düşürdü. Sonuç olarak, bu şekilde yara iyileşmesinin ilk dönemi olan yangısel dönemi kısaltarak, iyileşme sürecine olumlu etki yaptığı kanaatine varıldı. 

Anahtar Kelimeler

• Diabet, Hematolojik parametreler, Mineral maddeler, Rat, Yara iyileşmesi

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