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Abstract

In this case report, 8 fish samples taken from a commercial Rainbow trout farm during outbreak in the Black Sea Region were investigated. Causal agent of the outbreak was detected and its antibiotic susceptibility profile was investigated. The outbreak was observed at 21oC temperature in August, 2014. The 70-75 % mortality rate was caused by the agent in a month. Lactococcus garvieae were isolated from liver, spleen and kidney of 4 fish on the Tryptic Soy Agar, McConkey Agar and Shotts-Waltman Agar as the result of bacteriological examinations. Isolated strains were identified by biochemical tests and were confirmed by molecular methods. Similar phenotypic properties were shared by outbreak isolates. All the strains were sensitive to florfenicol, erythromycin, oxytetracycline, amoxicillin, ampicillin while all strains were resistant to trimethoprim, sulfamethoxazole/trimethoprim and oxolinic acid. The sensitivity of enrofloxacin, cefoperazone, kanamycin, neomycin, and gentamicin were determined at varying rates. Multidrug resistance was also detected in all the strains

Keywords

• Antibiotic susceptibility, Isolation, Lactococcus garvieae, PCR, Rainbow trout (Oncorhynchus mykiss)

Bir Alabalık Çiftliğinde Doğal Enfekte Gökkuşağı Alabalıklarından (Oncorhynchus mykiss Walbaum, 1792) Lactococcus garvieae’nin Kültür ve PCR ile Saptanması ve Etkenin Antibiyotik Duyarlılık Profillerinin Belirlenmesi

Abstract

Bu olgu sunumunda, Karadeniz Bölgesi’nde enfeksiyon çıkan bir ticari gökkuşağı alabalığı çiftliğinden toplanan 8 adet balık örneği incelendi. Hastalık etkeni saptandı ve etkenin antibiyotik duyarlılık profili araştırıldı. Salgın 21^oC su sıcaklığında ve 2014 yılı Ağustos ayı içerisinde görüldü. Enfeksiyon bir aylık süre içerisinde % 70-75 oranında mortaliteye neden oldu. Bakteriyolojik incelemeler sonucunda 4 balığın karaciğer, dalak ve böbreklerinden Tryptic Soy Agar, McConkey Agar ve Shotts-Waltman Agar’da Lactococcus garvieae izole edildi. Suşların identifikasyonu kültür tekniği ile konfirmasyonu ise moleküler yöntemlerle yapıldı. Çalışmada elde edilen izolatların fenotipik özelliklerinin birbirleriyle uyumlu olduğu saptandı. Suşların tamamı florfenikol, eritromisin, oksitetrasiklin, amoksisilin ve ampisiline duyarlı, trimetoprim, sulfametoksazol/trimetoprim ve okzolinik asite dirençli bulundu. Enrofloksasin, sefoperazon, kanamisin, neomisin, ve gentamisine karşı değişen oranlarda duyarlılık belirlenirken suşların tamamında çoklu antibiyotik direnci tespit edildi.

Keywords

• Antibiyotik duyarlılık, Gökkuşağı alabalığı (Oncorhynchus mykiss), İzolasyon, Lactococcus garvieae, PZR

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