Siklofosfamidle İndüklenmiş Ratlarda Serbest Radikaller ve Antioksidanlar Üzerine Naringinin Protektif Etkileri

Öz

Bu çalışmada siklofosfamid (CYP) ile uygulanan ratlarda kan ve serum örneklerinde Naringin (NA)’in antioksidanlar ve serbest radikaller üzerine protektif etkilerinin araştırılması amaçlandı. Araştırmamızda yaklaşık 200-250 g ağırlığında, 40 adet erkek erişkin Sprague Dawley rat kullanıldı ve her bir grupta 8 ratın bulunduğu 5 grup oluşturuldu. Kontrol grubuna 10 gün boyunca intra gastrik (i.g.) serum fizyolojik (1 ml) verildi. CYP grubuna 10 gün boyunca i.g. serum fizyolojik verildi ve 10. gün tek doz intra peritonal (i.p.) CYP (200 mg/kg) enjekte edildi. NA50+CYP ve NA100+CYP gruplarına 10 gün boyunca sırasıyla serum fizyolojikte çözdürülmüş 50 ve 100 mg/kg dozunda i.g. NA uygulandı ve NA’nın son enjeksiyondan sonra tek doz CYP (200 mg/kg, i.p.) enjekte edildi. NA100 grubuna ise 10 gün boyunca 100 mg/kg dozunda i.g. NA uygulandı. Deneyin 12. gününde ratlar tiopental sodyum (20 mg/kg) ile anestezi altına alınarak intra kardiyak kan örnekleri alındı ve dekapite edildiler. Ratlardan elde edilen kan ve serum örneklerinde Total Antioksidan Durumu (TAS), Total Oksidan Durumu (TOS), Oksidatif stres indeksi (OSİ) ve 8-Hydroxydeoxyguanosine (8-OHdG) parametreleri analiz edildi. CYP’nin ratlarda oksidatif stresi artırdığı, antioksidanları baskıladığı ve DNA hasarına yol açtığı tespit edildi. NA’nın farklı dozlarının, CYP’nin sebep olduğu oksidatif stresi baskıladığı, antioksidan miktarını artırdığı ve DNA hasarını önlediği belirlendi.

Anahtar Kelimeler

• Antioksidan
• Naringin
• Rat
• Serbest Radikal
• Siklofosfamid

Protective Effects of Naringin on Free Radicals and Antioxidants in Cyclophosphamide Induced Rats

Abstract

In this study, it was aimed to investigate the protective effects of Naringin (NA) on antioxidants and free radicals in blood and serum samples in rats administered with cyclophosphamide (CYP). In our study, 40 male adult Sprague Dawley rats weighing about 200-250 g were used and 5 groups were formed with 8 rats in each group. The control group was given to intra-gastric (i.g.) saline (1 ml) for 10 days. The CYP group was given i.g. saline for 10 days and on the 10th day, a single dose of intraperitoneal (i.p.) CYP (200 mg/kg) was injected. NA50+CYP and NA100+CYP groups were administered NA at a dose of 50 and 100 mg/kg dissolved in saline for 10 days, respectively and and single dose CYP (200 mg/kg, i.p.) after NA last injection was injected. On the 12th day of the experiment, the rats were anesthetized with thiopental sodium (20 mg/kg) and intra cardiac blood samples were taken and they were decapitated. Total Antioxidant Status (TAS), Total Oxidant Status (TOS), Oxidative stress index (OSI) and 8-Hydroxydeoxyguanosine (8-OHdG) parameters in the blood and serum samples obtained from the rats were analyzed. It was determined that CYP increases oxidative stress in rats, suppresses antioxidants and causes DNA damage. It was determined that different doses of NA suppress oxidative stress caused by CYP, increase the amount of antioxidants and prevent DNA damage.

Keywords

• Antioxidant
• Cyclophosphamide
• Free radical
• Naringin
• Rat

References

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