Effects of Rutin on 5-Fluorouracil-Induced Nephrotoxicity in Rats

Abstract

5-Fluorouracil (5-FU) is a widely used agent in chemotherapy. This study was aimed to investigate the effects of Rutin on 5-FU-induced nephrotoxicity in rats. In our study, 40 male adult rats were used and the rats were divided into five groups as Control, 5-FU, Rutin50+5-FU, Rutin100+5-FU, and Rutin100. According to the experimental protocol, rats were administered 50 and 100 mg/kg doses of Rutin and a single dose (200 mg/kg) of 5-FU for 14 days. On the 15th day of the experimental study, intracardiac blood samples were taken from rats under anesthesia, and rats were decapitated to obtain kidney tissue samples. Urea, creatinine, and blood urea nitrogen (BUN) values in serum samples were analyzed in an autoanalyzer. Kidney tissues were detected in 10% formalin and hematoxylin-eosin (HE) staining was performed. Histopathological examination was performed to determine the effects of 5-FU and Rutin application on kidney tissue. In the 5-FU group; serum urea, creatinine and BUN levels increased significantly compared to other groups. It was observed that an especially high dose of Rutin prevented 5-FU-induced increases in kidney function tests and histopathological changes caused by 5-FU in kidney tissue. In consequence of this study, it was determined that Rutin significantly prevented 5-FU-induced kidney toxicity in rats.

Keywords

• 5-Fluorouracil
• Nephrotoxicity
• Rat
• Rutin

Sıçanlarda 5-Florourasil ile İndüklenen Nefrotoksisitede Rutin’in Etkileri

Öz

5-Florourasil (5-FU) kemoterapide yaygın olarak kullanılan bir ajandır. Bu çalışmada, sıçanlarda 5-FU ile indüklenen nefrotoksisitede Rutin’in etkilerinin araştırılması amaçlandı. Çalışmamızda 40 adet erkek erişkin sıçan kullanıldı ve sıçanlar Kontrol, 5-FU, Rutin50+5-FU, Rutin100+5-FU ve Rutin100 olmak üzere beş gruba ayrıldı. Deneysel protokole göre sıçanlara, 14 gün boyunca Rutin’in 50 ve 100 mg/kg dozları ve tek doz (200 mg/kg) 5-FU uygulandı. Deneysel çalışmanın 15. gününde sıçanlardan anestezi altında intrakardiyak kan örnekleri alındı ve böbrek doku örneklerinin alınması için sıçanlar dekapite edildi. Serum örneklerinde üre, kreatinin ve kan üre nitrojen (BUN) değerleri otoanalizörde analiz edildi. Böbrek dokuları %10’luk formalinde tespit edildi ve hematoksilen-eozin (HE) boyaması yapıldı. Böbrek dokusunda 5-FU ve Rutin uygulamasının etkilerinin belirlenmesi amacıyla histopatolojik inceleme yapıldı. 5-FU grubunda; serum üre, kreatinin ve BUN seviyeleri diğer gruplara göre anlamlı düzeyde arttı. Rutin’in özellikle yüksek dozu, böbrek fonksiyon testlerinde 5-FU kaynaklı artışları ve 5- FU’nun böbrek dokusunda meydana getirmiş olduğu histopatolojik değişiklikleri önlediği görüldü. Bu çalışmanın sonucunda, Rutin’in sıçanlarda 5-FU ile indüklenen böbrek toksisitesini önemli ölçüde önlediği belirlendi.

Anahtar Kelimeler

• 5-Florourasil
• nefrotoksisite
• rutin
• Sıçan

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