Investigations on Clinic, Haematology, Biochemistry, Oxidative Stress, Acute Phase Proteins in Infectious Respiratory Disease Complex (BRDC) in Cattle
Abstract
In this study, clinical status,
treatment, acute phase proteins, oxidant and antioxidant status, changes in
serum biochemistry and hematological parameters in Bovine Respiratory Disease
Complex (BRDC) in cattle were investigated. A total of 30 cattle were used in
the study. The sick animals included in the study were divided into two groups.
Group I (n = 10) given tulathromycin + flunixin-meglumine + Vitamin E and
selenium combination. Group II (n = 10) given tulathromycin + flunixin-meglumine.
Blood samples were collected one time from the control group (n = 10), and four
times (pre-treatment and in 1, 3, 5 days of post-treatment) from the patient
groups. Serum acute phase proteins (Hp and SAA), biochemical and hematological
analyzes, total antioxidant (TAS) and total oxidant (TOS) levels were
determined from all samples. Pre-treatment (day 0) serum Fe and Ca
concentrations in both disease groups were significantly (P<0.05) lower compared to the control group. It was
observed that these parameters improved parallel to clinical improvement with
treatment. It was determined that the total leukocyte counts of the patient
groups were significantly higher than the control group before the treatment.
In both treatment groups, pre-treatment serum amyloid A and haptaglobulin
levels and TOS levels were higher than the control group (P<0.05). In conclusion, it was found
that significant increases occurred in serum Hp and SAA concentrations in acute
phase proteins and serum TOS concentrations in oxidative stress parameters in
Bovine Respiratory Disease Complex, and that there were significant hematologic
and biochemical changes in this disease.
Keywords
References
- 1. Blood DC., Radostits OM., 1994. Veterinary Medicine, 8th ed., 749-758, Bailliere-Tindall, London.
- 2. Jericho KWF., Kozub GC., 2004. Experimental infectious respiratory disease in groups of calves: Lobar distribution, variance, and sample-size requirements for vaccine evaluation. Can J Vet Res, 68, 118-127.
- 3. Martin SW., Lumsden JH., 1987. The relationship of hematology and serum biochemistry parameters to treatment for respiratory disease and weight gain in Ontario feedlot calves. Can J Vet Res, 51, 499-505.
- 4. Verhoeff J., Van der Ban M., Van Nieuwstadt AP., 1984. Bovine respiratorysyncytial virus infections in young dairy cattle: clinical and haematological findings. Vet Rec, 114, 9-12.
- 5. Nakajima Y., Momotani E., Murakami T., Ishikawa Y., Morimatsu M., Saito M., Suzuki H., Yasukawa K., 1993. Induction of acute phase protein by recombinant human interleukin-6 (IL-6) in calves. Vet Immunol Immunopathol, 35, 385-391.
- 6. Petersen HH., Nielsen JP., Heegaard MH., 2004. Application of acute phase protein measurements in veterinary clinical chemistry. Vet Res, 35,163-187.
- 7. Murata H., Shimada N., Yoshioka M., 2004. Current research on acute phase proteins in veterinary diagnosis: an overview. Vet J, 168, 28-40.
- 8. Conner JG., Eckersall PD., 1988. Bovine and Canine Acute Phase Proteins Vet Res Commun, 12, 169-178.
- 9. Sies H., 1997. İmpaired endothelial and smooth muscle cell function ın oxidative stress. Exp Physiol, 82, 291-295. 10. Carter GR., 1984. Genus I Pasteurella In “Bergey’s Manual of Systematic Bacteriology”, Ed., Krig N.R., Holt JG, 552-558, Williams and Wilkins, Baltimore, USA.
- 11. Holt JG., Kreig NR., Sneath PHA., Staley JT., Williams ST., 1994. Bergey’s Manual of Determinative Bacteriology. Ed., Hensley W.R., 196, Williams and Wilkins, Baltimore, USA.
- 12. Allen JW., Viel L., Bateman KG., Rosental S., Shewen PE., Sheard PP., 1991. The microbial flora of respiratory tract in feedlot calves: Associations between nasopharyngial and broncoalveolar lavage cultures. Can J Vet Res, 55, 341-346.
- 13. Angen O., Thomsen J., Larsen LE., Larsen J., Kokotovic B., Heegard PMH., Enemark JMD., 2009. Respiratory diseases in calves: Microbiological investigations on trans-tracheally aspirated bronchoalveolar fluid and acute phase protein response. Vet Microbiol, 137, 165-171.
- 14. Al-Qudah KM., 2009. Oxidative stress in calves with acute or chronic bronchopneumonia. Rev Med Vet, 160, 5, 231-236.
- 15. Gökce HI., Woldehivet Z., 1999. The effects of Ehrlichia (Cytoecetes) phagochytophila on the Clinical chemistry of sheep and goats. J Vet Med, 46, 93-103.
- 16. Smith JE., Debowes RM., Cipriano JE., 1986. Exogenous corticosteroids increase serum iron concentrations in mature horses and ponies, J Am Vet Med Assoc, 188, 1296-1298.
- 17. Talkhan OFA., Salama SEM., Kholy MME., Mosallam SA., Atwa EI., 2009. Bacterial agent of respiratory manifestation in cattle and associated biochemical alterations in Menoufiea Governorate. Nature Sci, 7, 26-30.
- 18. Godson DL., Campos M., Attah-Poku SK., Redmond MJ., Cordeiro DM., Sehti MS., Harland RJ., Babiuk LA., 1996. Serum haptoglobin as an indicator of the acute phase response in bovine respiratory disease. Vet Immunol Immunopathol, 51, 277-292. 19. Nikunen S., hartel H., Orro T., Neuvonen E., Tanskanen R., Kivela SL., Sankari S., Aho P., Pyörala S., Saloniemi H., Soveri T., 2007. Association of bovine respiratory disease with clinical status and acute phase proteins. Comp Immunol Microbiol Infec Dis, 30, 143-151.
- 20. Heegaard PMH., Godson DL., Toussaint MJM., Tjornehoj K., Larsen LE., Viuff B., Ronsholt L., 2000. The acute phase response of haptoglobin and serum amyloid A (SAA) in cattle undergoing experimental infection with bovine respiratory syncytial virus. Vet Immunol Immunopathol, 77, 151-159.
- 21. Valarcher JF., Taylor G., 2007. Bovine respiratory syncytial virus infection. Vet Res, 38, 153-158.
Sığırlarda Enfeksiyöz Solunum Sistemi Hastalıkları Kompleksinde (BRDC) Klinik, Hematoloji, Biyokimya, Oksidatif Stres, Akut Faz Proteinler Üzerinde Araştırmalar
Abstract
Bu çalışmada,
sığırlarda solunum sistemi kompleksi (BRDC) hastalığında klinik, tedavi, akut
faz proteinler, oksidant ve anti oksidant durumu, serum biyokimyası ve
hematolojik parametrelerdeki değişmeler araştırıldı. Çalışmada toplam 30 sığır
kullanıldı. Çalışma kapsamına alınan hasta hayvanlar iki gruba ayrıldı. I.
Gruptaki hayvanlara (n=10), tulatromycin +Flunixin meglumin + Vitamin E ve
selenyum kombinasyonu uygulandı. II. gruptaki hayvanlara (n=10) tulatromycin+
flunixin meglumin uygulandı. Kontrol grubundan (n=10) tek sefer, hasta
gruplarda tedavi öncesi (0. gün) ve tedavi sonrası 1, 3 ve 5. günlerde kan ve
serum örnekleri alınarak biyokimyasal, hematolojik analizlerle serum akut faz
proteinleri (Hp ve SAA), Total antioksidant (TAS) ve Total oksidant düzeyleri
(TOS) ölçüldü. Hasta grupların her ikisinde de serum Fe, Ca
konsantrasyonlarının tedavi öncesinde (0. gün) kontrol grubuna göre önemli
derecede (P<0.05) düşük olduğu belirlendi. Bu parametrelerin tedaviyle
birlikte klinik iyileşmeye paralel olarak düzeldiği gözlendi. Hasta grupların
tedavi öncesinde total lökosit sayılarının kontrol grubuna göre önemli derecede
yüksek olduğu belirlendi. Her iki tedavi grubundaki hayvanlarda tedavi öncesi
serum amyloid A ve haptoglobulin düzeyleri ile TOS değerlerinin kontrol grubuna
göre yüksek olduğu belirlendi (P<0.05). Sonuç olarak, sığırlarda solunum
sistemi hastalıkları kompleksinde hematolojik ve biyokimyasal değişimlerin
olduğu ve özellikle de akut faz proteinlerinden serum Hp ve SAA ile oksidatif
stres parametrelerinden serum TOS konsantrasyonlarında önemli artışların olduğu
saptandı.
Keywords
References
- 1. Blood DC., Radostits OM., 1994. Veterinary Medicine, 8th ed., 749-758, Bailliere-Tindall, London.
- 2. Jericho KWF., Kozub GC., 2004. Experimental infectious respiratory disease in groups of calves: Lobar distribution, variance, and sample-size requirements for vaccine evaluation. Can J Vet Res, 68, 118-127.
- 3. Martin SW., Lumsden JH., 1987. The relationship of hematology and serum biochemistry parameters to treatment for respiratory disease and weight gain in Ontario feedlot calves. Can J Vet Res, 51, 499-505.
- 4. Verhoeff J., Van der Ban M., Van Nieuwstadt AP., 1984. Bovine respiratorysyncytial virus infections in young dairy cattle: clinical and haematological findings. Vet Rec, 114, 9-12.
- 5. Nakajima Y., Momotani E., Murakami T., Ishikawa Y., Morimatsu M., Saito M., Suzuki H., Yasukawa K., 1993. Induction of acute phase protein by recombinant human interleukin-6 (IL-6) in calves. Vet Immunol Immunopathol, 35, 385-391.
- 6. Petersen HH., Nielsen JP., Heegaard MH., 2004. Application of acute phase protein measurements in veterinary clinical chemistry. Vet Res, 35,163-187.
- 7. Murata H., Shimada N., Yoshioka M., 2004. Current research on acute phase proteins in veterinary diagnosis: an overview. Vet J, 168, 28-40.
- 8. Conner JG., Eckersall PD., 1988. Bovine and Canine Acute Phase Proteins Vet Res Commun, 12, 169-178.
- 9. Sies H., 1997. İmpaired endothelial and smooth muscle cell function ın oxidative stress. Exp Physiol, 82, 291-295. 10. Carter GR., 1984. Genus I Pasteurella In “Bergey’s Manual of Systematic Bacteriology”, Ed., Krig N.R., Holt JG, 552-558, Williams and Wilkins, Baltimore, USA.
- 11. Holt JG., Kreig NR., Sneath PHA., Staley JT., Williams ST., 1994. Bergey’s Manual of Determinative Bacteriology. Ed., Hensley W.R., 196, Williams and Wilkins, Baltimore, USA.
- 12. Allen JW., Viel L., Bateman KG., Rosental S., Shewen PE., Sheard PP., 1991. The microbial flora of respiratory tract in feedlot calves: Associations between nasopharyngial and broncoalveolar lavage cultures. Can J Vet Res, 55, 341-346.
- 13. Angen O., Thomsen J., Larsen LE., Larsen J., Kokotovic B., Heegard PMH., Enemark JMD., 2009. Respiratory diseases in calves: Microbiological investigations on trans-tracheally aspirated bronchoalveolar fluid and acute phase protein response. Vet Microbiol, 137, 165-171.
- 14. Al-Qudah KM., 2009. Oxidative stress in calves with acute or chronic bronchopneumonia. Rev Med Vet, 160, 5, 231-236.
- 15. Gökce HI., Woldehivet Z., 1999. The effects of Ehrlichia (Cytoecetes) phagochytophila on the Clinical chemistry of sheep and goats. J Vet Med, 46, 93-103.
- 16. Smith JE., Debowes RM., Cipriano JE., 1986. Exogenous corticosteroids increase serum iron concentrations in mature horses and ponies, J Am Vet Med Assoc, 188, 1296-1298.
- 17. Talkhan OFA., Salama SEM., Kholy MME., Mosallam SA., Atwa EI., 2009. Bacterial agent of respiratory manifestation in cattle and associated biochemical alterations in Menoufiea Governorate. Nature Sci, 7, 26-30.
- 18. Godson DL., Campos M., Attah-Poku SK., Redmond MJ., Cordeiro DM., Sehti MS., Harland RJ., Babiuk LA., 1996. Serum haptoglobin as an indicator of the acute phase response in bovine respiratory disease. Vet Immunol Immunopathol, 51, 277-292. 19. Nikunen S., hartel H., Orro T., Neuvonen E., Tanskanen R., Kivela SL., Sankari S., Aho P., Pyörala S., Saloniemi H., Soveri T., 2007. Association of bovine respiratory disease with clinical status and acute phase proteins. Comp Immunol Microbiol Infec Dis, 30, 143-151.
- 20. Heegaard PMH., Godson DL., Toussaint MJM., Tjornehoj K., Larsen LE., Viuff B., Ronsholt L., 2000. The acute phase response of haptoglobin and serum amyloid A (SAA) in cattle undergoing experimental infection with bovine respiratory syncytial virus. Vet Immunol Immunopathol, 77, 151-159.
- 21. Valarcher JF., Taylor G., 2007. Bovine respiratory syncytial virus infection. Vet Res, 38, 153-158.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Araştırma Makaleleri |
| Authors | |
| Publication Date | April 30, 2017 |
| Published in Issue | Year 2017 Volume: 12 Issue: 1 |