The effects of chloramphenicol, thiamphenicol and florfenicol on glucose 6-phosphate dehydrogenase (G6PD), reduced glutathione (GSH) enzyme activities and some haematological parameters in mice

Year 2007, Volume: 2 Issue: 4, 159 - 163, 18.04.2010

Ali Karadeniz Sinan İnce Harun Alp

Abstract

The aim of present study was to investigate the effects of chloramphenicol thiamphenicol and florfenicol on activities of reduced glutathione (GSH) and glucose-6 phosphate dehydrogenase (G6PD) enzymes that play an important role in the free radical metabolism of erythrocytes and haematological parameters in the mice. In the study, 70 male Swiss albino mice were used. Animals were divided into seven groups, each having 10 mice. Each antibiotic were given ad libitum in drinking water at levels of 0 (control), 100 mg/kg and 200 mg/kg for 7 days. Biochemical and haematological analysis were studied on days 1, 7 and following 14. Both dosages of chloramphenicol and thiamphenicol, but not florfenicol were induced a significant reduction (p < 0.05) in GSH and G6PD enzyme activities on days 7 and 14 compared to control group. Hovewer, only high dosage of thiamphenicol and both dosage of chloramphenicol were induced a significant (p < 0.05) decrease in red blood cell (RBC) count, hemoglobin (Hb) value, packed cell volume (PCV) , white blood cell (WBC) and neutrophil percentage on day 14 compared to control group. The present findings indicate that chloramphenicol and thiamphenicol have significant inhibition effects on the activities of erythrocytes GSH, G6PD enzymes and some haematological parameters in mice.

Keywords

Antibiotic, Glucose-6 phosphate dehydrogenase, Reduced glutathione, Haematological parameter, mouse

Farelerde glukoz-6 fosfat dehidrogenaz (G6PD), redükte glutatyon (GSH) enzim aktiviteleri ile bazı hematolojik parametreler üzerine kloramfenikol, tiamfenikol ve florfenikolün etkileri

Abstract

Bu çalışmanın amacı farelerde, eritrositlerin serbest radikal metabolizmasında rol oynayan
glukoz-6 fosfat dehidrogenaz (G6PD) ve glutatyon (GSH) enzim aktiviteleri ile kan değerleri üzerine
kloramfenikol, tiamfenikol ve florfenikolün etkilerinin araştırılmasıdır. Çalışmada 70 adet Swiss albino erkek
fare kullanıldı. Hayvanlar herbirinde 10 adet fare olacak şekilde 7 gruba ayrıldı. Her bir antibiyotik içme
suları ile ad libitum olarak 0 (kontrol), 100 mg/kg ve 200 mg/kg dozlarında 7 gün uygulandı. Biyokimyasal
ve hematolojik analizler uygulamanın 1. ve 7. günleri ile takip eden 14. günde yapıldı. Kloramfenikol ve
tiamfenikolün her iki dozu GSH ve G6PD enzim aktivitelerini kontrol grubuna oranla 7. ve 14. günlerde
istatistiksel olarak önemli oranda düşürürken (p < 0.05) florfenikolün etki yapmadığı tespit edildi. Bununla
birlikte tiamfenikolün sadece yüksek dozu ve kloramfenikolün ise her iki dozu kontrol grubu ile
karşılaştırıldığında 14. günde alyuvar sayısı (RBC), hemoglobin (Hb) düzeyi, hematokrit (PCV) değer,
akyuvar sayısı (WBC) ve nötrofil yüzde oranını önemli oranda azalttı (p < 0.05). Elde edilen bulgular
farelerde kloramfenikol ve tiamfenikolün eritrosit GSH ve G6PD enzim aktivitesi ile bazı hematolojik
parametreler üzerine engelleyici bir etkiye sahip olduğunu gösterdi.

Keywords

Antibiyotik, Glukoz-6 fosfat dehidrogenaz, Redükte glutatyon, Hematolojik parametre, Fare

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