Yetişkin Bıldırcınların Gastrointestinal Sisteminde Gastrin, Somatostatin ve Serotonin Salgılayan Hücreler Üzerine İmmunohistokimyasal Bir Araştırma

Year 2011, Volume: 6 Issue: 3, 183 - 193, 21.12.2011

Nejdet Şimşek , Ali Karadeniz Zekeriya Özüdoğru Adem Kara , İsmail Can

Abstract

Bu araştırmada, yetişkin bıldırcınların gastrointestinal sistemindeki gastrin (G hücreleri), somatostatin (D hücreleri) ve
serotonin (EC hücreleri) immunopozitif hücrelerin dağılımı, yerleşimi ve sıklığının immunohistokimyasal yöntemlerle
belirlenmesi amaçlandı. Çalışmada, 10 adet sağlıklı yetişkin bıldırcının glanduler ve musküler mideleri, duodenum, jejunum
ve ileumları materyal olarak kullanıldı. Alınan doku kesitleri streptavidin biotin peroksidaz yöntemi ile immunohistokimyasal
olarak boyandı. Gastrin ve serotonin immunopozitif hücrelerin glanduler ve musküler midelere göre ince barsaklarda daha
fazla olduğu belirlendi. Bununla birlikte, somatostatin immunopozitif hücreler mide ve ince barsakların tamamında çok az
sayıda görüldü. Ayrıca, G, D ve EC hücrelerinin her iki midede genellikle kapalı tip, ince barsak bölümlerinde ise hem açık
hem de kapalı tip endokrin hücreler halinde bulunduğu da tespit edildi. Sonuç olarak, bıldırcınların glanduler ve musküler
mideleri, duodenum, jejunum ve ileumlarında G, D ve EC hücrelerinin değişen sayılarda bulunduğu belirlendi. Ayrıca,
duodenum ve ileum mukozasında, G ve EC hücrelerinin D hücrelerine göre daha fazla bulunduğu da tespit edildi.

Keywords

Bıldırcın, Enteroendokrin hücreler, Gastrointestinal sistem, İmmunohistokimya

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