Kısraklarda Erken Gebelik Tespiti

Year 2012, Volume: 7 Issue: 1, 67 - 75, 25.04.2012

Mehmet Atlı Mehmet Köse Ercan Kurar Aydın Güzeloglu Ahmet Semacan

Abstract

At yetiştiriciliğinde kısa olan üreme sezonunun iyi değerlendirilip, sezon boşa geçirilmeden kısraklarda gebeliğin
oluşturulması öncelikli hedefler arasında yer almaktadır. Sınırlı üreme sezonuna sahip kısrakların ülkemizin de içinde
bulunduğu coğrafyada ilkbahar aylarında başlayan ovaryum aktiviteleri sonbahara kadar devam etmektedir. Bu süreç içinde
reprodüktif herhangi bir sorunu olmayan kısraklarda düzenli östrus siklusu sayısıda oldukça azdır. Dolayısıyla gebeliğin
mümkün olan en kısa zamanda tespitinin kritik önemi bulunmaktadır. Bu amaçla farklı tanı yöntemleri kullanılmasına
rağmen, gebeliğin mümkün olan en kısa zamanda teşhisine olanak sağlayacak etkin ve pratik bir yönteme halen ihtiyaç
duyulmaktadır. Bu derleme kapsamında öncelikle kısrakların erken gebelik fizyolojisi hakkında kısaca bilgi verilecektir.
Mevcut gebelik tanı yöntemleri (östrusların izlenmesi, rektal palpasyon, ultrasonografi, progesteron, östrojen, eCG, erken
gebelik faktörünün ölçümü ve gebeliğe bağlı gen ekspresyonları ile gebelik tespiti) avantajları ve dezavantajları açısından
tartışılacaktır. Ayrıca, gebeliğin maternal kabul mekanizmasının moleküler düzeyde anlaşılması amacıyla yapılan güncel
çalışmalar ve gebeliğin tanısında alternatif yaklaşımlar hakkında bilgi verilecektir.

Keywords

Erken gebelik tespiti, Kısrak

References

• Allen WR., 2000. The physiology of early pregnancy in the mare. Proc. Am. Assoc. Equine Pract., 40, 338-354.
• Allen WR., Antczak DF., 2000. Reproduction and modern breeding technologies in the mare. In “The Genetics of the Horse”, Eds., A Bowling, A Ruvinsky, CAB In- ternational, NY, USA.
• Allen WR., 2001. Luteal deficiency and embryo mortality in the mare. Reprod. Dom. Anim., 36, 121-131.
• Allen WR., 2005. Maternal recognition and maintenance of pregnancy in the mare. Anim. Reprod., 2, 209- 223.
• Allen WR., Wilsher S., 2009. A review of implantation and early placentation in the mare. Placenta, 30, 1005- 1015.
• Aslan S., Fındık M., Izgür H., Çelebi M., Çelebi M., 1997. Pregnancy diagnosis by using PMSG-Latex Test in mares. Tr. J. Vet. Anim. Sci., 21, 195-199.
• Atli MO., Kurar E., Kayis SA., Aslan S., Semacan A., Celik S., Guzeloglu A., 2010. Evaluation of genes involved in prostaglandin action in equine endometrium during estrous cycle and early pregnancy. Anim. Reprod. Sci.,122, 124-132.
• Atli MO., Guzeloglu A., Dinç DA., 2011. Expression of wingless type (WNT) genes and their antagonists at mRNA levels in equine endometrium during the es- trous cycle and early pregnancy. Anim. Reprod. Sci., 125, 94-102.
• Battut I., Colchen S., Fieni F., Tainturier D., Bruyas JF., 1997. Success rates when attempting to nonsurgi- cally collect equine embryos at 144, 156 or 168 hours after ovulation. Equine Vet. J., Suppl., 25, 60- 62.
• Bergfelt DR., Adams GP., 2007. Ovulation and corpus luteum development. In “Current Therapy in Equine Reproduction”, Eds., JC Samper, JF Pycock, AO McKinnon, W.B. Saunders Elsevier, USA.
• Collins AM., Buckley TC., 1993. Comparison of methods for early pregnancy detection. J. Equi. Vet. Sci., 13, 627-630.
• Cordoba MC., Sartori R., Fricke PM., 2001. Assessment of a commercially available early conception factor (ECF) test for determining pregnancy status of dairy cattle. J. Dairy Sci., 84, 1884-1889.
• Cross DT., Ginther OJ., 1988. Uterine contractions in nonpregnant and early pregnant mares and jennies as determined by ultrasonography. J. Anim. Sci., 66, 250-254.
• Daels PF., Shideler S., Lasley BL., Hughes JP., Stabenfeldt GH., 1990. Source of oestrogen in early pregnancy in the mare. J. Reprod. Fertil., 90, 55-61
• Daels PF., DeMoraes JJ., Stabenfeldt GH., Hughes JP., Lasley BL., 1991. The corpus luteum: source of oes- trogen during early pregnancy in the mare. J. Re- prod. Fertil., 44, 501-508.
• Daels PF., Hughes JP., 1993. The normal estrous cycle. In “Equine Reproduction”, Eds., AO Mckinnon, JL Voss JL, 1st edition, Lea & Febiger, Philadelphia, USA.
• Daels PF., Albrecht BA., Mohammed HO., 1998. Equine chorionic gonadotropin regulates luteal steroido- genesis in pregnant mares. Biol. Reprod., 59, 1062- 1068.
• Dixit VD., Parvizi N., 2000. Lymphocytic secretion of adrenocorticotropic hormone throughout preg- nancy in cows and its possible physiological role. Reprod. Domes. Anim., 3-4,171.
• Dixit VD., Parvizi N., 2001. Pregnancy stimulates secretion of adrenocorticotropin and nitric oxide from periph- eral bovine lymphocytes. Biol. Reprod., 64, 242-248.
• England GCW., 2005. Fertility and Obstetrics in the Horse. 3rd edition., Blackwell Publishing, Indian.
• Fay JE., Douglas RH., 1982. The use of radioreceptor assay fort he detection of pregnancy in the mare. Therio- genology, 18, 431-444.
• Foristall KM., Roser JF., Liu IKM., Lasley B., Munro CJ., Carneiro GF., 1998. Development of a Tandem Hor- mone Assay for the detection of pregnancy in the Miniature Mare. Proc. Am. Assoc. Equine Pract., 44, 52-53.
• Gandy B., Tucker W., Ryan P., Williams A., Tucker A., Moore A., Godfrey R., Willard S., 2001. Evaluation of the early conception factor (ECF) test for the detec- tion of nonpregnancy in dairy cattle. Theriogenol- ogy, 56, 637-647.
• Gifford CA., Racicot K., Clark DS., Austin KJ., Hansen TR., Lucy MC., Davies CJ., Ott TL., 2007. Regulation of interferon-stimulated genes in peripheral blood leu- kocytes in pregnant and bred, nonpregnant dairy cows. J. Dairy Sci., 90, 274–280.
• Ginther OJ., 1992. Reproductive biology of the mare. In “Basic and Applied Aspect”, Ed., OJ Ginther, USA Equieservices, Cross Plains, WI, USA.
• Ginther OJ., 1998. Equine pregnancy: physical interactions between the uterus and conceptus. Proc. Am. Assoc. Equine Pract., 73-104.
• Gordon I. Controlled Reproduction in Horses, Deer and Camelids. CAB International, UK. 99-118.
• Green JC., Okamura CS., Poock SE., Lucy MC., 2010. Measurement of interferon-tau (IFN-τ) stimulated gene expression in blood leukocytes for pregnancy diagnosis within 18–20 d after insemination in dairy cattle. Anim. Reprod. Sci., 121, 24–33.
• Gunther JD., Foley CW., Gaverick HA., Plotka ED., 1980. Comparison of milk and blood plasma progesterone concentrations in cycling and pregnant mares. J. Anim. Sci., 51, 1131-1138.
• Hicks BA., Etter SJ., Carnahan KG., Joyce MM., Assiri AA., Carling SJ., Kodali K., Johnson GA., Hansen TR., Mirando MA., Woods GL., Vanderwall DK., Ott TL., 2003. Expression of the uterine Mx protein in cyclic and pregnant cows, gilts, and mares. J. Anim. Sci., 81, 1552–1561.
• Hill JA., Choi BC., 2000. Immunodystrophism: evidence for a novel alloimmune hypothesis for recurrent preg- nancy loss involving Th1-type immunity to tro- phoblast. Semin. Reprod. Med., 18, 401-405.
• Horteloup MP., Threlfall WR., Funk JA., 2005. The early conception factor (ECFTM) lateral flow assay for non-pregnancy determination in the mare. Therio- genology, 64, 1061–1071.
• Hyland JH., 1990. Reproductive endocrinology: it’s role in fertility and infertility in the horse. Br. Vet. J., 146, 1- 16.
• Kahn W., 1992. Ultrasonograpy as a diagnostic tool in female animal reproduction. Anim. Reprod. Sci., 28, 1-10.
• Kahn W., 1994. Veterinary Reproductive Ultrasonography. 1st edition., Mosby-Wolfe, London, England.
• Kılıçarslan MR., Soylu MK., Şenünver A., Kırşan İ., Carioğlu B., 1996. The use of ultrasonografik techniques for the diagnosis of early pregnancy in mares. Kafkas Üniv. Vet. Fak. Derg., 2, 147-150.
• Klein C., Scoggin KE., Ealy AD., Troedsson MH., 2010. Transcriptional profiling of equine endometrium during the time of maternal recognition of preg- nancy. Biol. Reprod., 83, 102-113.
• Kurar E., Atlı MO., Güzeloğlu A., Semacan A., 2011. POMC, INOS, PGES, IL-4, IL-5 and IL-10 gene expression in peripheral blood mononuclear cells of cyclic and pregnant mares. Kafkas Üniv. Vet. Fak. Derg., 17, 319-323.
• Kurar E., Atli MO., Guzeloglu A., Kayıs SA., Semacan A., 2012. Expression of Toll-like receptors (TLRs) in the equine peripheral blood mononuclear cells (PMBCs) during the early pregnancy and estrous cycle. Euro- sian Vet. J., Article in press.
• McDowell KJ., Sharp DC., Grubaugh W., Thatcher WW., Wilcox CJ., 1988. Restricted conceptus mobility re- sults in failure of pregnancy maintenance in mares. Biol. Reprod., 39, 340–348.
• McKinnon AO., 1993. Diagnosis of Pregnancy. In “Equine Reproduction”, Eds., AO Mckinnon, JL Voss, Lea & Febiger, Philadelphia, USA.
• McKinnon AO., Pycock JF., 2007. Maintenance of pregnancy. In “Current Therapy in Equine Reproduc- tion”, Eds., JC Samper, JF Pycock, AO McKinnon, W.B. Saunders Elsevier, USA.
• Metcalf ES., McCue P., Jasko DJ., Roor JB., 2004. Evalua- tion of a test for equine early conception factor. 50th Annual Convention of the American Associa- tion of Equine Practitioners , Denver, Colorado, USA.
• Meyer F., Ramanujam KS., Gobert AP., James SP., Wilson KT., 2003. Cutting edge: cyclooxygenase-2 activation suppresses Th1 polarization in response to Helico- bacter pylori. J. Immunol., 171, 3913-3917.
• Mitchell D., 1971. Early fetal death and a serum gonad- otrophin test for pregnacy in the mares. Can. Vet. J., 12, 41-44.
• Nett TM., Holtan DW., Estergreen VL., 1975. Oestrogens, LH, PMSG, and prolactin in serum of pregnant mares. J. Reprod. Fertil, 23, 457-462.
• Nett TM., Picket EW., Seidel GE., Voss JL., 1976. Levels of luteinizing hormone and progesterone during the estrous cycle and early pregnancy in mares. Biol. Reprod., 14, 412-415.
• Ohnuma K., Yokoo M., Ito K., Nambo Y., Miyake YI., Komatsu M., Takahashi J., 2000. Study of early preg- nancy factor (EPF) in equine (Equus caballus). Am. J. Reprod. Immunol., 43, 174-1799.
• Oliveira JF., Henkes LE., Ashley RL., Purcell SH., Smirnova NP., Rao Veeramachaneni DN., Anthony RV., Hansen TR., 2008. Expression of interferon (IFN)-stimulated genes in extrauterine tissues during early pregnancy in sheep is the consequence of endocrine IFN-τ re- lease from the uterine vein. Endocrinology, 149, 1252–1259.
• Oriol JG., Sharom FJ, Betteridge KJ., 1993. Developmen- tally regulated changes in the glycoproteins of the equine embryonic capsule. J. Reprod. Fert., 99, 653– 664.
• Parker E., Tibary A., Vanderwall DK., 2005. Evaluation of a new early pregnancy test in mares. J. Equi. Vet. Sci., 25, 66-69.
• Pycock JF., 2007. Pregnancy diagnosis in the mare. “Current Therapy in Equine Reproduction”, Eds., JC Samper, JF Pycock, AO McKinnon, Saunders Elsevier, USA.
• Reinhard G., Noll A., Schlebusch H., Mallmann P., Ruecker AV., 1998. Shifts in the TH1/TH2 balance during hu- man pregnancy correlate with apoptotic changes. Biochem. Biophys. Res. Commun., 245, 933-938.
• Roberts SJ., 1986. Gestation and pregnancy diagnosis in the mare. In “Current Therapy in Theriogenology”, Ed., DA Marrow, WB. 1st edition Saunders Com- pany, Philadelphia, USA.
• Rooke J., Ewen M., McEvoy T., Entrican G., Ashworth C., 2005. Effect of inclusion of serum and granulocyte- macrophage colony stimulating factor on secretion of interferon-tau during the in vitro culture of ovine embryos. Reprod. Fertil. Dev., 17, 513-521.
• Roozendaal R., Vellenga E., Postma DS., De Monchy JG., Kauffman HF., 1999. Nitric oxide selectively de- creases interferon-gamma expression by activated human T lymphocytes via a cGMP-independent mechanism. Immunology, 98, 393-399.
• Sayegh AI., Adams SB., Peter AT., Wilson DG., 1996. Equine rectal tears: causes an management. Comp. Cont. Educ. Pract. Vet., 18, 1131-1139.
• Şenünver A., Horoz H., Kılıçarslan MR., Konuk SC., Tek Ç., Alkan S., 1995. Kısraklarda östrus ve ovulasyonun PGF2a ile uyarılması. Bültendif, 5, 11-14.
• Takagi M., Nishimura K., Oguri N., Ohnumo K., Ito K., Takahashi J., Yasuda Y., Miyazawa K., Sato K., 1998. Measurement of early pregnancy factor activity for monitoring the viability of the equine embryo. Theriogenology, 50, 255-262.
• Trebble TM., Wootton SA., Miles EA., Mullee M., Arden NK., Ballinger AB., Stroud MA., Burdge GC., Calder PC., 2003. Prostaglandin E2 production and T cell function after fish-oil supplementation: response to antioxidant cosupplementation. Am. J. Clin. Nutr., 78, 376-382.
• Walker W., Rotondo D., 2004. Prostaglandin E2 is a potent regulator of interleukin-12- and interleukin-18- induced natural killer cell interferon-gamma synthe- sis. Immunology, 111, 298-305.
• Weber JA., Freeman DA., Vanderwall DK., Woods GL., 1991. Prostaglandin E2 secretion by oviductal trans- port-stage equine embryos. Biol. Reprod., 45, 540- 543.
• Wegmann TG., Lin H., Guilbert L., Mosmann TR., 1993. Bidirectional cytokine interactions in the maternal- fetal relationship: is successful pregnancy a TH2 phenomenon. Immunol. Today, 14, 353-356.
• Wilder RL., 1998. Hormones, pregnancy and autoimmune diseases. Ann NY. Acad. Sci., 840, 45-50.