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Sıçanlarda Karbon Tetraklorürün (CCl4) Neden Olduğu Koagülasyon Bozukluklarında Etil Pirüvatın Etkisi

Year 2017, Volume: 12 Issue: 2, 137 - 142, 30.10.2017
https://doi.org/10.17094/ataunivbd.347965

Abstract

Çalışmada, sıçanlarda
karbon tetraklorürün (CCl4) neden olduğu koagülasyon bozukluklarında
etil pirüvatın (EP) etkisi araştırıldı. Kırk erkek Sprague Dawley ırkı sıçan
dört eşit gruba ayrıldı. Kontrol grubuna 1 ml Ringer laktat solüsyonu 0., 1,5.
ve 6. saatlerde periton içi verildi. Karbon tetraklorür grubuna 1.6 g/kg CCl4
periton içi yolla tek doz verilmesini takiben 1.5 ve 6 saat sonra 1 ml Ringer
laktat uygulandı. Etil pirüvat grubuna 40 mg/kg dozda etil pirüvat 0, 1.5 ve 6.
saatlerde periton içi enjekte edildi. Etil pirüvat+CCl4 grubuna ise
tek doz 1.6 g/kg CCl4 uygulamasından 30 dak. önce ve 1 ve 6 saat
sonra 40 mg/kg dozda etil pirüvat periton içi uygulandı. Karbon tetraklorür,
protrombin zamanı (PTZ) ve aktive parsiyel tromboplastin zamanı (aPTT)
değerlerinde uzama (P<0.05), fibrinojen düzeyinde azalma (P<0.05) ve alanin
aminotransferaz (ALT) aktivitesinde artma (P<0.05) oluşturdu. Öte yandan,
CCl4 grubuyla karşılaştırıldığında, CCl4 uygulamasından
30 dak. önce ve 1 ve 6 saat sonra 40 mg/kg dozda verilen etil pirüvat fibrinojen
düzeyini artırırken (P<0.05) PTZ ve aPTT sürelerinde ve ALT aktivitesinde
azalmaya (P<0.05) neden oldu. Sonuç olarak, etil pirüvatın sıçanlarda CCl4’ün
neden olduğu pıhtılaşma bozuklukları üzerine koruyucu bir etki gösterebileceği
tespit edildi. Etil pirüvatın bu etkisi, karaciğer hastalıklarıyla ilişkili
pıhtılaşma bozukluklarının önlenmesinde yararlı olabilir.

References

  • 1. O'Donnell-Tormey J., Nathan CF., Lanks K., DeBoer CJ., de la Harpe J., 1987. Secretion of pyruvate: an antioxidant defense of mammalian cells. J Exp Med, 165, 500–514.
  • 2. Uchiyama T., Delude RL., Fink MP., 2003. Dose-dependent effects of ethyl pyruvate in mice subjected to mesenteric ischemia and reperfusion. Intensive Care Med, 29, 2050-2058.
  • 3. Salahudeen AK., Clark EC., Nath KA., 1991. Hydrogen peroxide-induced renal injury. A protective role for pyruvate in vitro and in vivo. J Clin Investing, 88, 1886-1893.
  • 4. Bunger R., Mallet RT., Hartman DA., 1989. Pyruvate-enhanced phosphorylation potential and inotropism in normoxic and postischemic isolated working heart: near complete prevention of reperfusion contractile failure. Eur J Biochem, 180, 221-233.
  • 5. Cicalese L., Lee K., Schraut W., Watkins S., Borle A., Stanko R., 1999. Pyruvate prevents ischemia-reperfusion mucosal injury of rat small intestine. The Am J Surg, 171, 97-100.
  • 6. Sileri P., Schena S., Morini S., Rastellini C., Pham S., Benedetti E., Cicalese L., 2001. Pyruvate inhibits hepatic ischemi reperfusion injury in rats. Transplant, 72, 27-30.
  • 7. Tawadrous ZS., Delude RL., Fink MP., 2002. Resuscitation from hemorrhagic shock with Ringer’s ethyl pyruvate solution improves survival and ameliorates intestinal mucosal hyperpermeability in rats. Shock, 17, 473-477.
  • 8. Ulloa L., Ochani M., Yang H., Tanovic M., Halperin D., Yang R., Czura CJ., Fink MP., Tracey KJ., 2002. Ethyl pyruvate prevents lethality in mice with established lethal sepsis and systemic inflammation. PNAS, 99, 12351-12356.
  • 9. Yang R., Uchiyama T., Alber SM., Han X., Watkins SK., Delude RL., Fink MP., 2004. Ethyl pyruvate ameliorates distant organ injury in a murine model of acute necrotizing pancreatitis. Crit Care Med, 32, 1453-1459.
  • 10. Epperly M., Jin S., Nie S., Cao S., Zhang X., Franicola D., Wang H., Fink MP., Greenberger JS., 2007. Ethyl pyruvate, a potentially effective mitigator of damage after total-body irradiation. Radiat Res, 168, 552-559.
  • 11. Wang LV., Wang LK., Chen H., Fan C., Li X., He CM., Gong ZJ., 2012. Ethyl pyruvate protects against experimental acute-on-chronic liver failure in rats, World J Gastroenterol, 18, 5709-5718.
  • 12. Hatch GE., Santrock J., Slade R., Hayes JM., 1988. Detection of CCL4-induced oxidation of hepatic tissue in vivo by oxigen-18 tracing. Toxicol Appl Pharmacol, 93, 81-88.
  • 13. Okazaki M., Zhang LS., Suzuki M., Sakamoto K., 1988. The measurement of platelet aggregation and ATP-release in mice with liver damage induced by carbontetrachloride (CCl4) using a whole blood aggregometer. Jpn J Pharmacol, 48, 407-15.
  • 14. Okazaki M., Furuya E., Shin Y., Sakamoto K., 1986. Studies on alterations in blood coagulative and fibrinolytic activities after single and multiple administrations of carbontetrachloride in mice. Jpn J Pharmacol, 41, 447-458.
  • 15. Vazquez C., Bujan J., Vallejo D., 1990. Blood coagulation variations induced by carbontetrachloride inhalation in Wistar rats. Toxicol Appl Pharmacol, 103, 206-213.
  • 16. Sakamoto K., Otsuka K., Kasahara T., Abe K., 1986. Effect of carbontetrachloride on blood coagulation and fibrinolytic activities in rats. Nihon Yakurigaku Zasshi, 88, 255-262.
  • 17. Haro C., Lazarte S., Zelaya H., Alvarez S., Agüero G., 2009. Yogurt: Effect on leukocytes and blood coagulation in an acute liver injury model. J Med Food, 12, 796-802.
  • 18. Arıcı OF., Çetin N., 2011. Protective role of ghrelin against carbontetrachloride (CCl4)- induced coagulation disturbances in rats. Regul Pept, 166, 139-142.
  • 19. Çetin E., Kanbur M., Çetin N., Eraslan G., Atasever A., 2011. Hepatoprotective effect of ghrelin on carbontetrachloride-induced acuteliver injury in rats. Regul Pept, 171, 1-5.
  • 20. Lee L., Kwon W., Jo Y., Suh G., Youn Y., 2008. Protective effects of ethyl pyruvate treatment on paraquat-intoxicated rats. Hum Exp Toxicol, 27, 49-54.
  • 21. Amitrano L., Guardascione MA., Brancaccio V., Balzano A., 2002. Coagulation disorders in liver disease. Semin Liver Dis, 22, 83-96.
  • 22. Ishii H., Kuboki M., Ohkura M., Hiraishi S., Tsubouchi J., Kazama M., 1985. Effect of intraperitoneal injection of topical thrombin on the coagulation and fibrinolysis of rabbits with experimental liver damages. Nihon Yakurigaku Zasshi, 85, 97-110.
  • 23. Nieuwenhuizen W., Emeis JJ., Vermond A., Kurver P., van der Heide D., 1980. Studies on the catabolisms and distribution of fibrinogen in rats. Biochem Biophys Res Comm, 97, 49-55.
  • 24. Kelly DA., Tuddenham EG., 1986. Haemostatic problems in liver disease. Gut, 27, 339-349.
  • 25. Kung CW., Lee YM., Yen MH., 2011. In vivo anticoagulan effect of ethyl pyruvate in endotoxemic rats. Thromb Res, 127, 582-588.
  • 26. Dong W., Cai B., Pena G., Pisarenko V., Vida G., Doucet D., Lee M., Sharpe S., Lu Q., Xu DZ., Ramos L., Deitch EA., Ulloa L., 2010. Ethyl pyruvate prevents inflammatory responses and organ damage during resuscitation in porcine hemorrhage. Shock, 34, 205-213.
  • 27. Duncan JR., Prasse KW., Mahaffey EA., 1986. Erythrocytes. 2nd ed., 7-9, Veterinary laboratory medicine clinical pathology, Lowa State University Press, Ames, Lowa.
  • 28. Yoshiyuki T., Yoshikazu O., Takemi Y., 2006. Effects of two formulations of combination drug (liver extract and flavin adenine dinucleotide sodium), acelart and adelavin, on carbontetrachloride-induced experimental hepatic dysfunction and weight in riboflavin deficient rats. Jpn Pharmacol Ther, 34, 669-674.
  • 29. Wang W., Zhao F., Fang Y., Li X., Shen L., Coa T., Zhu H.,2013. Glycyrrhizin protects against porcine endotoxemia through modulation of systemic inflammatory response. Crit Care, 17, R44.

Effect of Ethyl Pyruvate on Carbon Tetrachloride (CCl4)-Induced Coagulation Disturbances in Rats

Year 2017, Volume: 12 Issue: 2, 137 - 142, 30.10.2017
https://doi.org/10.17094/ataunivbd.347965

Abstract

The effect of ethyl pyruvate (EP)
on carbon tetrachloride (CCl4)-induced haemostatic disturbances in
rats was investigated in this study. Forty male Sprague-Dawley rats were
divided into four equal groups.
The control group was given intraperitoneally 1 ml
of Ringer’s lactate solution at 0 h, 1,5 h and 6 hours
. The
CCl4 group was treated intraperitoneally with a single dose of 1.6
g/kg CCl4 followed by the administration of 1 ml Ringer’s lactate
solution at at 1,5 and 6 hours
. The
ethyl pyruvate group was injected intraperitoneally with ethyl pyruvate at a
dose of 40 mg/kg at 0, 1,5 and 6 hours. The ethyl pyruvate +CCl4
group were administered intraperitoneally with ethyl pyruvate at a dose of 40
mg/kg at 30 min before and at 1 and 6 h after a single dose of 1.6 g/kg CCl4
injection
.Treatment of CCl4 prolonged prothrombin time (PT) and activated
thromboplastin time (aPTT), decreased fibrinogen level and increased alanine
aminotranspherase (ALT) activity. On the other hand, the administration of EP
intraperitoneally at 30 minutes before, and at 1.5
and 6 h after CCl4 significantly (P<0.05) decreased PT, aPTT
and ALT values and increased (P<0.05) fibrinogen level when compared with
CCl4-treated only group. The results of our study suggest that ethyl
pyruvate treatment plays a protective role on CCl4-induced
coagulation disturbances in rats. This effect of ethyl pyruvate may be useful
for preventing haemostatic disturbances associated with liver diseases

References

  • 1. O'Donnell-Tormey J., Nathan CF., Lanks K., DeBoer CJ., de la Harpe J., 1987. Secretion of pyruvate: an antioxidant defense of mammalian cells. J Exp Med, 165, 500–514.
  • 2. Uchiyama T., Delude RL., Fink MP., 2003. Dose-dependent effects of ethyl pyruvate in mice subjected to mesenteric ischemia and reperfusion. Intensive Care Med, 29, 2050-2058.
  • 3. Salahudeen AK., Clark EC., Nath KA., 1991. Hydrogen peroxide-induced renal injury. A protective role for pyruvate in vitro and in vivo. J Clin Investing, 88, 1886-1893.
  • 4. Bunger R., Mallet RT., Hartman DA., 1989. Pyruvate-enhanced phosphorylation potential and inotropism in normoxic and postischemic isolated working heart: near complete prevention of reperfusion contractile failure. Eur J Biochem, 180, 221-233.
  • 5. Cicalese L., Lee K., Schraut W., Watkins S., Borle A., Stanko R., 1999. Pyruvate prevents ischemia-reperfusion mucosal injury of rat small intestine. The Am J Surg, 171, 97-100.
  • 6. Sileri P., Schena S., Morini S., Rastellini C., Pham S., Benedetti E., Cicalese L., 2001. Pyruvate inhibits hepatic ischemi reperfusion injury in rats. Transplant, 72, 27-30.
  • 7. Tawadrous ZS., Delude RL., Fink MP., 2002. Resuscitation from hemorrhagic shock with Ringer’s ethyl pyruvate solution improves survival and ameliorates intestinal mucosal hyperpermeability in rats. Shock, 17, 473-477.
  • 8. Ulloa L., Ochani M., Yang H., Tanovic M., Halperin D., Yang R., Czura CJ., Fink MP., Tracey KJ., 2002. Ethyl pyruvate prevents lethality in mice with established lethal sepsis and systemic inflammation. PNAS, 99, 12351-12356.
  • 9. Yang R., Uchiyama T., Alber SM., Han X., Watkins SK., Delude RL., Fink MP., 2004. Ethyl pyruvate ameliorates distant organ injury in a murine model of acute necrotizing pancreatitis. Crit Care Med, 32, 1453-1459.
  • 10. Epperly M., Jin S., Nie S., Cao S., Zhang X., Franicola D., Wang H., Fink MP., Greenberger JS., 2007. Ethyl pyruvate, a potentially effective mitigator of damage after total-body irradiation. Radiat Res, 168, 552-559.
  • 11. Wang LV., Wang LK., Chen H., Fan C., Li X., He CM., Gong ZJ., 2012. Ethyl pyruvate protects against experimental acute-on-chronic liver failure in rats, World J Gastroenterol, 18, 5709-5718.
  • 12. Hatch GE., Santrock J., Slade R., Hayes JM., 1988. Detection of CCL4-induced oxidation of hepatic tissue in vivo by oxigen-18 tracing. Toxicol Appl Pharmacol, 93, 81-88.
  • 13. Okazaki M., Zhang LS., Suzuki M., Sakamoto K., 1988. The measurement of platelet aggregation and ATP-release in mice with liver damage induced by carbontetrachloride (CCl4) using a whole blood aggregometer. Jpn J Pharmacol, 48, 407-15.
  • 14. Okazaki M., Furuya E., Shin Y., Sakamoto K., 1986. Studies on alterations in blood coagulative and fibrinolytic activities after single and multiple administrations of carbontetrachloride in mice. Jpn J Pharmacol, 41, 447-458.
  • 15. Vazquez C., Bujan J., Vallejo D., 1990. Blood coagulation variations induced by carbontetrachloride inhalation in Wistar rats. Toxicol Appl Pharmacol, 103, 206-213.
  • 16. Sakamoto K., Otsuka K., Kasahara T., Abe K., 1986. Effect of carbontetrachloride on blood coagulation and fibrinolytic activities in rats. Nihon Yakurigaku Zasshi, 88, 255-262.
  • 17. Haro C., Lazarte S., Zelaya H., Alvarez S., Agüero G., 2009. Yogurt: Effect on leukocytes and blood coagulation in an acute liver injury model. J Med Food, 12, 796-802.
  • 18. Arıcı OF., Çetin N., 2011. Protective role of ghrelin against carbontetrachloride (CCl4)- induced coagulation disturbances in rats. Regul Pept, 166, 139-142.
  • 19. Çetin E., Kanbur M., Çetin N., Eraslan G., Atasever A., 2011. Hepatoprotective effect of ghrelin on carbontetrachloride-induced acuteliver injury in rats. Regul Pept, 171, 1-5.
  • 20. Lee L., Kwon W., Jo Y., Suh G., Youn Y., 2008. Protective effects of ethyl pyruvate treatment on paraquat-intoxicated rats. Hum Exp Toxicol, 27, 49-54.
  • 21. Amitrano L., Guardascione MA., Brancaccio V., Balzano A., 2002. Coagulation disorders in liver disease. Semin Liver Dis, 22, 83-96.
  • 22. Ishii H., Kuboki M., Ohkura M., Hiraishi S., Tsubouchi J., Kazama M., 1985. Effect of intraperitoneal injection of topical thrombin on the coagulation and fibrinolysis of rabbits with experimental liver damages. Nihon Yakurigaku Zasshi, 85, 97-110.
  • 23. Nieuwenhuizen W., Emeis JJ., Vermond A., Kurver P., van der Heide D., 1980. Studies on the catabolisms and distribution of fibrinogen in rats. Biochem Biophys Res Comm, 97, 49-55.
  • 24. Kelly DA., Tuddenham EG., 1986. Haemostatic problems in liver disease. Gut, 27, 339-349.
  • 25. Kung CW., Lee YM., Yen MH., 2011. In vivo anticoagulan effect of ethyl pyruvate in endotoxemic rats. Thromb Res, 127, 582-588.
  • 26. Dong W., Cai B., Pena G., Pisarenko V., Vida G., Doucet D., Lee M., Sharpe S., Lu Q., Xu DZ., Ramos L., Deitch EA., Ulloa L., 2010. Ethyl pyruvate prevents inflammatory responses and organ damage during resuscitation in porcine hemorrhage. Shock, 34, 205-213.
  • 27. Duncan JR., Prasse KW., Mahaffey EA., 1986. Erythrocytes. 2nd ed., 7-9, Veterinary laboratory medicine clinical pathology, Lowa State University Press, Ames, Lowa.
  • 28. Yoshiyuki T., Yoshikazu O., Takemi Y., 2006. Effects of two formulations of combination drug (liver extract and flavin adenine dinucleotide sodium), acelart and adelavin, on carbontetrachloride-induced experimental hepatic dysfunction and weight in riboflavin deficient rats. Jpn Pharmacol Ther, 34, 669-674.
  • 29. Wang W., Zhao F., Fang Y., Li X., Shen L., Coa T., Zhu H.,2013. Glycyrrhizin protects against porcine endotoxemia through modulation of systemic inflammatory response. Crit Care, 17, R44.
There are 29 citations in total.

Details

Journal Section Araştırma Makaleleri
Authors

Ebru Çetin

Publication Date October 30, 2017
Published in Issue Year 2017 Volume: 12 Issue: 2

Cite

APA Çetin, E. (2017). Sıçanlarda Karbon Tetraklorürün (CCl4) Neden Olduğu Koagülasyon Bozukluklarında Etil Pirüvatın Etkisi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 12(2), 137-142. https://doi.org/10.17094/ataunivbd.347965
AMA Çetin E. Sıçanlarda Karbon Tetraklorürün (CCl4) Neden Olduğu Koagülasyon Bozukluklarında Etil Pirüvatın Etkisi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. October 2017;12(2):137-142. doi:10.17094/ataunivbd.347965
Chicago Çetin, Ebru. “Sıçanlarda Karbon Tetraklorürün (CCl4) Neden Olduğu Koagülasyon Bozukluklarında Etil Pirüvatın Etkisi”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 12, no. 2 (October 2017): 137-42. https://doi.org/10.17094/ataunivbd.347965.
EndNote Çetin E (October 1, 2017) Sıçanlarda Karbon Tetraklorürün (CCl4) Neden Olduğu Koagülasyon Bozukluklarında Etil Pirüvatın Etkisi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 12 2 137–142.
IEEE E. Çetin, “Sıçanlarda Karbon Tetraklorürün (CCl4) Neden Olduğu Koagülasyon Bozukluklarında Etil Pirüvatın Etkisi”, Atatürk Üniversitesi Veteriner Bilimleri Dergisi, vol. 12, no. 2, pp. 137–142, 2017, doi: 10.17094/ataunivbd.347965.
ISNAD Çetin, Ebru. “Sıçanlarda Karbon Tetraklorürün (CCl4) Neden Olduğu Koagülasyon Bozukluklarında Etil Pirüvatın Etkisi”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 12/2 (October 2017), 137-142. https://doi.org/10.17094/ataunivbd.347965.
JAMA Çetin E. Sıçanlarda Karbon Tetraklorürün (CCl4) Neden Olduğu Koagülasyon Bozukluklarında Etil Pirüvatın Etkisi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2017;12:137–142.
MLA Çetin, Ebru. “Sıçanlarda Karbon Tetraklorürün (CCl4) Neden Olduğu Koagülasyon Bozukluklarında Etil Pirüvatın Etkisi”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, vol. 12, no. 2, 2017, pp. 137-42, doi:10.17094/ataunivbd.347965.
Vancouver Çetin E. Sıçanlarda Karbon Tetraklorürün (CCl4) Neden Olduğu Koagülasyon Bozukluklarında Etil Pirüvatın Etkisi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2017;12(2):137-42.