Sıçanlarda Karbon Tetraklorürün (CCl4) Neden Olduğu Koagülasyon Bozukluklarında Etil Pirüvatın Etkisi

Year 2017, Volume: 12 Issue: 2, 137 - 142, 30.10.2017

Ebru Çetin

https://doi.org/10.17094/ataunivbd.347965

Abstract

Çalışmada, sıçanlarda
karbon tetraklorürün (CCl₄) neden olduğu koagülasyon bozukluklarında
etil pirüvatın (EP) etkisi araştırıldı. Kırk erkek Sprague Dawley ırkı sıçan
dört eşit gruba ayrıldı. Kontrol grubuna 1 ml Ringer laktat solüsyonu 0., 1,5.
ve 6. saatlerde periton içi verildi. Karbon tetraklorür grubuna 1.6 g/kg CCl₄
periton içi yolla tek doz verilmesini takiben 1.5 ve 6 saat sonra 1 ml Ringer
laktat uygulandı. Etil pirüvat grubuna 40 mg/kg dozda etil pirüvat 0, 1.5 ve 6.
saatlerde periton içi enjekte edildi. Etil pirüvat+CCl₄ grubuna ise
tek doz 1.6 g/kg CCl₄ uygulamasından 30 dak. önce ve 1 ve 6 saat
sonra 40 mg/kg dozda etil pirüvat periton içi uygulandı. Karbon tetraklorür,
protrombin zamanı (PTZ) ve aktive parsiyel tromboplastin zamanı (aPTT)
değerlerinde uzama (P<0.05), fibrinojen düzeyinde azalma (P<0.05) ve alanin
aminotransferaz (ALT) aktivitesinde artma (P<0.05) oluşturdu. Öte yandan,
CCl₄ grubuyla karşılaştırıldığında, CCl₄ uygulamasından
30 dak. önce ve 1 ve 6 saat sonra 40 mg/kg dozda verilen etil pirüvat fibrinojen
düzeyini artırırken (P<0.05) PTZ ve aPTT sürelerinde ve ALT aktivitesinde
azalmaya (P<0.05) neden oldu. Sonuç olarak, etil pirüvatın sıçanlarda CCl₄’ün
neden olduğu pıhtılaşma bozuklukları üzerine koruyucu bir etki gösterebileceği
tespit edildi. Etil pirüvatın bu etkisi, karaciğer hastalıklarıyla ilişkili
pıhtılaşma bozukluklarının önlenmesinde yararlı olabilir.

Keywords

Etil pirüvat, Karbon tetraklorür, Pıhtılaşma bozuklukları

Effect of Ethyl Pyruvate on Carbon Tetrachloride (CCl4)-Induced Coagulation Disturbances in Rats

Abstract

The effect of ethyl pyruvate (EP)
on carbon tetrachloride (CCl₄)-induced haemostatic disturbances in
rats was investigated in this study. Forty male Sprague-Dawley rats were
divided into four equal groups. The control group was given intraperitoneally 1 ml
of Ringer’s lactate solution at 0 h, 1,5 h and 6 hours. The
CCl₄ group was treated intraperitoneally with a single dose of 1.6
g/kg CCl₄ followed by the administration of 1 ml Ringer’s lactate
solution at at 1,5 and 6 hours. The
ethyl pyruvate group was injected intraperitoneally with ethyl pyruvate at a
dose of 40 mg/kg at 0, 1,5 and 6 hours. The ethyl pyruvate +CCl₄
group were administered intraperitoneally with ethyl pyruvate at a dose of 40
mg/kg at 30 min before and at 1 and 6 h after a single dose of 1.6 g/kg CCl₄
injection.Treatment of CCl₄ prolonged prothrombin time (PT) and activated
thromboplastin time (aPTT), decreased fibrinogen level and increased alanine
aminotranspherase (ALT) activity. On the other hand, the administration of EP
intraperitoneally at 30 minutes before, and at 1.5 and 6 h after CCl₄ significantly (P<0.05) decreased PT, aPTT
and ALT values and increased (P<0.05) fibrinogen level when compared with
CCl₄-treated only group. The results of our study suggest that ethyl
pyruvate treatment plays a protective role on CCl₄-induced
coagulation disturbances in rats. This effect of ethyl pyruvate may be useful
for preventing haemostatic disturbances associated with liver diseases

Keywords

Carbon tetrachloride, Coagulation disorders, Ethyl pyruvate

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