Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus

Year 2018, Volume: 13 Issue: 2, 128 - 134, 25.10.2018

Hasan Ceyhun Macun , Miyase Çınar Ahmet Kürşat Azkur , Hakan Kalender Serkan Erat

https://doi.org/10.17094/ataunivbd.315776

Cited By: 5

Abstract

The aim of this study was to determine oxidant-antioxidant status by evaluating some oxidative stress parameters (nitric oxide (NO), malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS), catalase activity) in clinically healthy Akkaraman ewes with seropositive for Schmallenberg virus (SBV). The study consisted of four positive, six suspected and seven negative-control blood sera in terms of specific antibody for SBV by using commercial ELISA kits. Even though there were no significant statistical differences (P>0.05), the highest NO level was in sera with SBV specific antibody positive while the lowest TOS level was in sera with SBV specific antibody negative. The values of TAS in sera were very similar in the groups (P=0.981). While the MDA levels of sera with SBV specific antibody positive and suspected were similar, the statistical difference was found between the MDA level of sera with SBV specific antibody positive and negative (P=0.021). The catalase activity of sera with SBV specific antibody positive was lower (P=0.030) than the catalase activity of sera with SBV suspected whereas the catalase activities in positive and negative groups and the catalase activities in suspected and negative groups were similar. In conclusion, it was determined that MDA level was affected in Akkaraman ewes that are SBV specific antibody positive without clinical symptoms.

Keywords

Oxidative stress, Schmallenberg virus, sheep

Schmallenberg Virüs Seropozitif Akkaraman Koyunlarda Oksidatif Stres

Abstract

Bu çalışmanın amacı Schmallenberg virüs (SBV) seropozitif belirlenen klinik olarak sağlıklı Akkaraman koyunlarda bazı oksidatif stres parametrelerinin (nitrik oksit (NO), malondialdehid (MDA), total antioksidan kapasite (TAK), total oksidan kapasite (TOK), katalaz aktivitesi) değerlendirilmesiyle oksidan-antioksidan durumun belirlenmesidir. Çalışmanın materyalini, ELISA yöntemiyle SBV özgül antikorları yönünden 4 pozitif, 6 şüpheli ve 7 negatif-kontrol kan serumu oluşturdu. Gruplar arasında istatistiksel bir fark olmamasına rağmen (P>0.05) en yüksek NO seviyesi SBV seropozitif serumlarındayken, en düşük TOK seviyesi SBV negatif serumlarda bulundu. Gruplardaki TAS değerleri oldukça benzerdi (P=0.981). SBV seropozitif ve şüpheli serum MDA seviyeleri benzerken SBV seropozitif ve negatif serumların MDA seviyeleri istatistiki olarak farklı bulundu (P=0.021). SBV seropozitif olan serumların katalaz aktiviteleri, SBV şüpheli serumların katalaz aktivitelerinden daha düşük (P=0.030) iken pozitif ve negatif grupların katalaz aktiviteleri ile şüpheli ve negatif grupların katalaz aktiviteleri benzer bulundu. Sonuç olarak; hastalığa ait klinik semptom bulunmayan SBV seropozitif Akkaraman ırkı hayvanlarda MDA seviyesinin etkilendiği belirlendi.

Keywords

Koyun, oksidatif stres, Schmallenberg virüs

References

• 1. Hoffmann B., Scheuch M., Hoper D., Jungblut R., Holsteg M., Schirrmeier H., Eschbaumer M., Goller KV., Wernike K., Fischer M., Breithaupt A., Mettenleiter TC., Beer M., 2012. Novel orthobunyavirus in cattle, Europe, 2011. Emerg Infect Dis, 18, 469-472.
• 2. Veldhuis AMB., van Schaik G., Vellema P., Elbers ARW., Bouwstra R., van der Heijden HMJF., Mars MH., 2013. Schmallenberg virus epidemic in the Netherlands: Spatiotemporal introduction in 2011 and seroprevalance in ruminants. Prev Vet Med, 112, 35-47.
• 3. Azkur AK., Tonbak Ş., 2015. Schmallenberg virüsü. F Ü Sağ Bil Vet Derg, 29, 129-135.
• 4. Helmer C., Eibach R., Humann-Ziehank E., Tegtmeyer PC., Burstel D., Mayer K., Moog U., Stauch S., Strobel H., Voigt K., Sieber P., Greiner M., Ganter M., 2016. Seroprevalence of Schmallenberg virus infection in sheep and goats flocks in Germany, 2012-2013. VMS, 2, 10-22.
• 5. Lievaart-Peterson K., Luttikholt SJM., Van den Brom R., Vellema P., 2012. Schmallenberg virus infection in small ruminants – First review of the situation and prospects in Northern Europe. Small Ruminant Res, 106, 71-76.
• 6. Muskens J., Smolenaars AJG., van der Poel WHM., van Wuijckhuise L., Holzhauer M., van Weering H., Kock P., 2012. Diarree en productiedaling op Nederlandse melkveebedrijven door het Schmallenbergvirus. Tijdschr Diergeneeskd, 137, 112-115.
• 7. Hechinger S., Wernike K., Beer M., 2013. Evaluating the protective efficacy of a trivalent vaccine containing Akabane virus, Aino virus and Chuzan virus against Schmallenberg virus infection. Vet Res, 44, 114.
• 8. Martinelle L., Dal Pozzo F., Gauthier B., Kirschvink N., Saegerman C., 2014. Field veterinary survey on clinical and economic impact of Schmallenberg virus in Belgium. Transbound Emerg Dis, 61, 285-288.
• 9. Méroc E., De Regge N., Riocreux F., Caij AB., van den Berg T., van der Stede Y., 2014. Distribution of Schmallenberg virus and seroprevalence in Belgian sheep and goats. Transbound Emerg Dis, 61, 425-431.
• 10. Sies H., 1997. Oxidative stress: Oxidants and antioxidants. Exp Physiol, 82, 291-295.
• 11. Lykkesfeldt J., Svendsen O., 2007. Oxidants and antioxidants in disease: Oxidative stress in farm animals. Vet J, 173, 502-511.
• 12. Matsufuji H., Ochi H., Shibamoto T., 2006. Formation and inhibition of genotoxic malonaldehyde from DNA oxidation controlled with EDTA. Food Chem Toxicol, 44, 236-241.
• 13. Han SN., Meydani M., Wu D., Bender BS., Smith DE., Viña J., Cao G., Prior RL., Meydani SN., 2000. Effect of long-term dietary antioxidant supplementation on influenza virus infection. J Gerontol A Biol Sci Med Sci, 55, B496-503.
• 14. Aydin H., Yildiz G., Engin A., Yilmaz A., Çelik K., Bakir S., 2010. Malondialdehyde, vitamin E, and anti-oxidant enzyme activity levels in patients with crimean-congo hemorrhagic fever. AJMR, 4, 2402-2409.
• 15. Narayanan A., Popova T., Turell M., Kidd J., Chertow J., Popov SG., Bailey C., Kashanchi F., Kehn-Hall K., 2011. Alteration in superoxide dismutase 1 causes oxidative stress and p38 MAPK activation following RVFV infection. PLoS One, 6, e20354.
• 16. Bozukluhan K., Atakisi E., Atakisi O., 2013. Nitric oxide levels, total antioxidant and oxidant capacity in cattle with foot-and-mouth-disease. Kafkas Univ Vet Fak Derg, 19, 179-181.
• 17. Camini FC., Almeida LT., Bernardes CS., Silva M., Pedrosa ML., Costa DC., de Lima WG., do Amaral Pinto C., Ferreira PCP., de Magalhães JC., de Brito Magalhães CL., 2014. Caraparu virus induces damage and alterations in antioxidant defenses in the liver of BALB/c mice after subcutaneous infection. Arch Virol, 159, 2621-2632.
• 18. Kirmizigul AH., Ogun M., Ozen H., Erkilic EE., Gokce E., Karaman M., Kukurt A., 2016. Oxidative stress and total sialic acid levels in sheep naturally infected with pox virus. Pak Vet J, 36, 312-315.
• 19. Uzlu E., Karapehlivan M., Erdoğan HM., Kızıltepe Ş., Erkılıç EE., Deveci HA., Gökçe E., Kaya İ., Çitil M., 2016. Serum and saliva sialic and oxidative stress parameters changes in bulls with foot and mouth disease. Kafkas Univ Vet Fak Derg, 22, 321-325.
• 20. Deveci AH., Kükürt A., Uzlu E., Sözdutmaz İ., Merhan O., Aktaş S., Alpay M., Kaya İ., Karapehlivan M., 2017. Evaluation of paraoxonase activity, total sialic acid and oxidative stress in sheep with ecthyma contagiosa. Kafkas Univ Vet Fak Derg, 23, 453-457.
• 21. Akkuş İ., 1995. Serbest Radikaller ve Fizyopatolojik Etkileri. 1st ed., 42-112, Mimoza Yayınları, Konya.
• 22. Gutteridge JMC., 1995. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem, 41, 1819-1828.
• 23. Bozukluhan K., Merhan O., Öğün M., Cihan M., Gökçe G., 2016. Omfalitisli buzağılarda bazı oksidatif stres parametre düzeylerinin belirlenmesi. F Ü Sağ Bil Vet Derg, 30, 79-81.
• 24. Özbek Ö., 2013. Testis iskemi/reperfüzyonu oluşturulmuş ratlarda apocyninin testis dokusu üzerine koruyucu ve tedavi edici etkilerinin araştırılması. İnönü Üniversitesi, Tıp Fakültesi, Türkiye.
• 25. Çaylak E., 2011. Hayvan ve bitkilerde oksidatif stres ile antioksidanlar. TAD, 9, 73-83.
• 26. Buege JA., Aust SD., 1978. Microsomal lipid peroxidation. Methods Enzymol, 52, 302–310
• 27. Miranda KM., Espey MG., Wink DA., 2001. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide, 5, 62–71.
• 28. Issi M., Gul Y., Yilmaz S., 2008. Clinical, haematological and antioxidant status in naturally poxvirus infected sheep. Revue Méd Vét, 159, 54-58.
• 29. Aytekin I., Aksit H., Sait A., Kaya F., Aksit D., Gokmen M., Unsal Baca A., 2015. Evaluation of oxidative stress via total antioxidant status, sialic acid, malondialdehyde and RT-PCR findings in sheep affected with bluetongue. Vet Rec Open, 2:e000054.
• 30. Atakisi E., Bozukluhan K., Atakisi O., Gokce HI., 2010. Total oxidant and antioxidant capacities and nitric oxide levels in cattle with traumatic reticuloperitonitis. Vet Rec, 167, 908-909.
• 31. Van der Poel WHM., Parlevliet JM., Verstraten ERAM., Kooi EA., Hakze-van der Honing R., Stockhofe N., 2014. Schmallenberg virus detection in bovine semen after experimental infection of bulls. Epidemiol Infect, 142, 1495-1500.
• 32. Kataria AK., Kataria N., 2012. Evaluation of oxidative stress in sheep affected with Paste des petits ruminants. JSPB, 8, 72-77.