Some Clinicopathological Some Clinicopathological Changes in Buffalo Calves Infected with Enterotoxigenic E. coli (ETEC) K99+ Detected by Direct ELISA in Buffalo Calves Infected with Enterotoxigenic E. coli (ETEC) K99+ Detected by Direct ELISA
Abstract
The objective of recurrent study was to determine the some clinicopathological (hematological and biochemical) changes in native buffalo calves (aged between 1-15 day) infected with enterotoxigenic E. coli (ETEC) K99+ detected by direct ELISA, during the period between September 2018- April 2019. A total of 40 fecal samples (30 samples from infected animal and 10 samples from clinically healthy Buffalo calves) was examined by direct ELISA. Six samples form diarrheic animals (20%) were positive for E. coli (K99). The blood gas analysis of blood samples revealed significant decrease in the means of the blood pH, Partial pressure of oxygen concentration (PO2 ), Partial pressure of carbon dioxide concentration (PCO2), base excess (BE), as well as electrolytes: sodium (Na+), chloride (CL-), bicarbonate (HCO3-), calcium (Ca++) and nonionized calcium (nCa++) ions concentrations, while a significant increase in the mean of hemoglobin concentration (tHB), packed cell volume (PCV) and anion gap (AG) in the buffalo calfs infected with enterotoxigenic E. coli (ETEC) K99+comparison with the control animals. The main clinicopathological changes in native buffalo calves infected with enterotoxigenic E. coli (ETEC) K99+ were significantly decrease in electrolytes, blood gases, Calcium (Ca++) and nonionized Calcium (nCa++) ions concentrations
Supporting Institution
College of Veterinary Medicine ,University of Mosul
References
- 1. Radostits OM., Gay CC., Hinchcliff KW., Constable PD., 2007. Veterinary. Medicine. A text book of disease of cattle, sheep, pigs and horse 10th ed., Saunders Elsevier, Edinburgh, pp, 847-871. 2. Constable PD., Hinchcliff KW., Done SH., Grünberg W., 2017. Veterinary medicine: A textbook of the diseases of cattle, sheep, pigs, goats and horses. 11th ed. Vol. 2 Elsevier Health Sciences, pp, 1879-1899. 3. Kaper JB., Nataro JP., Mobley HL., 2004. Pathogenic Escherichia coli. Nat Rev Microbiol, 2, 123-140. 4. Hosein HI., Azzam RA., Abo-Elwafa M., Menshawy AMS., Rouby S., 2019. Virulence profile of enteropathogenic escherichia coli (epec) isolated from the cases of neonatal calf diarrhea. Adv Anim Vet Sci, 7, 755-760. 5. Acres SD., 1985. Enterotoxigenic Escherichia coli infections in newborn calves: a review. J Dairy Sci, 68, 229-235. 6. Hosein HI., 2018. Infectious Diseases of domestic animals. Text book, Fourth edition, Lazer center for publication and distribution, Beni Suef, Egypt. 7. Cho Y., Yoon KJ., 2014. An overview of calf diarrhea-infectious etiology,diagnosis, and intervention. J Vet Sci, 15, 1-17. 8. Rana N., Raut AA., Khurana SK., Manuja A., Saini A., 2012. Isolation and biotyping of Salmonella and Escherichia coli associated with neonatal buffalo calves. Indian J Anim Sci, 82, 676-678. 9. Bashahun GM., Amina A., 2017. Colibacillosis in calves: A review of literature. J Anim Sci Vet Med, 2, 62-71. 10. Niaz B., Khan AMT., Javed AH., Khalid MA., 2000. Hematological studies in induced buffalo neonatal calves diarrhea with enteropathogenic E. coli. Pakistan Vet J, 20, 85-89. 11. Ghanem MM., El-Fkhrany SF., Abd El-Raof YM., El-Attar HM., 2012. Clinical and haematobiochemical evaluation of diarrheic neonatal buffalo calves (Bubalas Bubalis) with reference to antioxidant changes. Benha Vet Med J, 23, 275-288. 12. Khan JA., Khan MS., Khan MA., Avais M., Maqbool A., Salman M., Rehman Z., 2009. Epidemiology of major bacterial and viral causes of diarrheoa in Buffalo calves in three districts of the Punjab province of Pakistan. Pakistan J Zool (Supplementary Series) 9, 187-193. 13. Sherwood D., Snodgrass DR., Lawson GHK., 1993. Prevalence of Escherichia coli in calves in Scotland and northern England. Vet Res, 113, 208-212. 14. Al-Mafraji AMR., 2009. Detection of E. coli K99 and Rota virus antigens in diarrheic and healthy buffalo of Babil Province, Iraq. Al-Anbar J Vet Sci, 7, 17-21. 15. Baxby D., Blundell N., Hart CA., 1984. The development and performance of a simple, sensitive method for the detection of Cryptosporidium oocysts in faeces. J Hyg, 93, 317-323. 16. Borriello G., Lucibelli MG., De Carlo E., Auriemma C., Cozza D., Ascione G., Scognamiglio F., Iovane G., Galiero G., 2012. Characterization of enterotoxigenic E. coli (ETEC), Shiga-toxin producing E. coli (STEC) and necrotoxigenic E. coli (NTEC) isolated from diarrhoeic Mediterranean water buffalo calves (Bubalusbubalis). Res Vet Sci, 93, 18-22. 17. Gruenberg W., 2016. Overview of Coli-septicemia. Merck Manual, 11th Edn. Merck Sharp & Dohme Corp. USA. 18. Srivani M., Reddy Y., Narasimha Subramanyam KV., Lakshman M., Kavitha KL., Ramanipushpa RN., 2019. Prevalence, molecular characterization and antimicrobial resistance of enterotoxigenic Escherichia coli in diarrhoeic buffalo calves. Indian J of Comp Microbiol Immunol Infect Dis, 40, 37-41. 19. Coles EH., 1986. Veterinary clinical pathology. 4th ed. Saunders Company, Philadelphia, pp, 203-240. 20. Shekhar S., Ranjan R., Singh CV., Kumar P., 2017. Prevalence, clinicohaemato-biochemical alterations in colibacillosis in neonatal calves. Int J Current Microbiol App Sci, 9, 3192-3198. 21. Bashir S., Ashraf I., Dar AM., Majid A., Bhat AA., Farooq J., 2015. Isolation and detection of Escherichia coli from diarrheic calves along with biochemical and hematologic parameters. J. Cell Tissue Res., 15,2,5059-61. 22. Malik S., Kumar A., Verma AK., Gupta MK., Sharma SD., Sharma AK., Rahal A., 2013.Haematological profile and blood chemistry in diarrhoeic calves affected with collibacillosis. J Anim Health Prod, 1, 10-14
Direkt ELISA ile Tanısı Konmuş, Enterotoksijenik E. coli (ETEC) K99+ ile Enfekte Manda Yavrularındaki (Malak) Bazı Klinikopatolojik Değişiklikler
Abstract
Bu çalışmanın amacı, Eylül 2018 - Nisan 2019 tarihleri arasında, direkt ELISA ile Enterotoksijenik E. coli (ETEC) K99+ tespit edilen doğal buffalo buzağılarındaki (1-15 gün arası) klinikopatolojik (hematolojik ve biyokimyasal) bazı değişiklikleri belirlemekti. Toplam 40 dışkı örneği (enfekte hayvandan 30 örnek ve klinik olarak sağlıklı Buffalo buzağılarından 10 örnek) direkt ELISA ile incelendi. Diyareik hayvanlardan alınan altı numune (%20), E. coli (ETEC) K99+ için pozitifti. Kontrol grubundaki hayvanlarla karşılatırıldığında, Enterotoksijenik E. coli (ETEC) K99+ ile enfekte olmuş hayvanların kan örneklerindeki kan gazı analizi kan pH'ı, kısmi oksijen konsantrasyonu basıncı (PO2), kısmi karbondioksit konsantrasyonu (PCO2), baz fazlalığı (BE) ve ayrıca elektrolitler: sodyum (Na+), klorür (CL-), bikarbonat (HCO3-), Kalsiyum (Ca++) ve iyonlaştırılmamış Kalsiyum (nCa++) iyonları konsantrasyonlarında önemli azalmaya, hemoglobin konsantrasyonu (tHB), hematokrit (PCV) ve anyon açığı (AG) değerlerinde ise önemli bir artışa neden oldu. Enterotoksijenik E. coli (ETEC) K99+ ile enfekte olmuş doğal buffalo buzağılarındaki ana klinikopatolojik değişikliklerden; elektrolitler, kangazları, Kalsiyum (Ca++) ve iyonize olmayan Kalsiyum (nCa++) iyonları konsantrasyonları önemli ölçüd eazaldı.The main clinicopathological changes in native
buffalo calves infected with
enterotoxigenic E. coli (ETEC) K99+ were significant decrease in electrolytes, blood gases,Calcium
(Ca++) and nonionized Calcium (nCa++) ions concentrations.
References
- 1. Radostits OM., Gay CC., Hinchcliff KW., Constable PD., 2007. Veterinary. Medicine. A text book of disease of cattle, sheep, pigs and horse 10th ed., Saunders Elsevier, Edinburgh, pp, 847-871. 2. Constable PD., Hinchcliff KW., Done SH., Grünberg W., 2017. Veterinary medicine: A textbook of the diseases of cattle, sheep, pigs, goats and horses. 11th ed. Vol. 2 Elsevier Health Sciences, pp, 1879-1899. 3. Kaper JB., Nataro JP., Mobley HL., 2004. Pathogenic Escherichia coli. Nat Rev Microbiol, 2, 123-140. 4. Hosein HI., Azzam RA., Abo-Elwafa M., Menshawy AMS., Rouby S., 2019. Virulence profile of enteropathogenic escherichia coli (epec) isolated from the cases of neonatal calf diarrhea. Adv Anim Vet Sci, 7, 755-760. 5. Acres SD., 1985. Enterotoxigenic Escherichia coli infections in newborn calves: a review. J Dairy Sci, 68, 229-235. 6. Hosein HI., 2018. Infectious Diseases of domestic animals. Text book, Fourth edition, Lazer center for publication and distribution, Beni Suef, Egypt. 7. Cho Y., Yoon KJ., 2014. An overview of calf diarrhea-infectious etiology,diagnosis, and intervention. J Vet Sci, 15, 1-17. 8. Rana N., Raut AA., Khurana SK., Manuja A., Saini A., 2012. Isolation and biotyping of Salmonella and Escherichia coli associated with neonatal buffalo calves. Indian J Anim Sci, 82, 676-678. 9. Bashahun GM., Amina A., 2017. Colibacillosis in calves: A review of literature. J Anim Sci Vet Med, 2, 62-71. 10. Niaz B., Khan AMT., Javed AH., Khalid MA., 2000. Hematological studies in induced buffalo neonatal calves diarrhea with enteropathogenic E. coli. Pakistan Vet J, 20, 85-89. 11. Ghanem MM., El-Fkhrany SF., Abd El-Raof YM., El-Attar HM., 2012. Clinical and haematobiochemical evaluation of diarrheic neonatal buffalo calves (Bubalas Bubalis) with reference to antioxidant changes. Benha Vet Med J, 23, 275-288. 12. Khan JA., Khan MS., Khan MA., Avais M., Maqbool A., Salman M., Rehman Z., 2009. Epidemiology of major bacterial and viral causes of diarrheoa in Buffalo calves in three districts of the Punjab province of Pakistan. Pakistan J Zool (Supplementary Series) 9, 187-193. 13. Sherwood D., Snodgrass DR., Lawson GHK., 1993. Prevalence of Escherichia coli in calves in Scotland and northern England. Vet Res, 113, 208-212. 14. Al-Mafraji AMR., 2009. Detection of E. coli K99 and Rota virus antigens in diarrheic and healthy buffalo of Babil Province, Iraq. Al-Anbar J Vet Sci, 7, 17-21. 15. Baxby D., Blundell N., Hart CA., 1984. The development and performance of a simple, sensitive method for the detection of Cryptosporidium oocysts in faeces. J Hyg, 93, 317-323. 16. Borriello G., Lucibelli MG., De Carlo E., Auriemma C., Cozza D., Ascione G., Scognamiglio F., Iovane G., Galiero G., 2012. Characterization of enterotoxigenic E. coli (ETEC), Shiga-toxin producing E. coli (STEC) and necrotoxigenic E. coli (NTEC) isolated from diarrhoeic Mediterranean water buffalo calves (Bubalusbubalis). Res Vet Sci, 93, 18-22. 17. Gruenberg W., 2016. Overview of Coli-septicemia. Merck Manual, 11th Edn. Merck Sharp & Dohme Corp. USA. 18. Srivani M., Reddy Y., Narasimha Subramanyam KV., Lakshman M., Kavitha KL., Ramanipushpa RN., 2019. Prevalence, molecular characterization and antimicrobial resistance of enterotoxigenic Escherichia coli in diarrhoeic buffalo calves. Indian J of Comp Microbiol Immunol Infect Dis, 40, 37-41. 19. Coles EH., 1986. Veterinary clinical pathology. 4th ed. Saunders Company, Philadelphia, pp, 203-240. 20. Shekhar S., Ranjan R., Singh CV., Kumar P., 2017. Prevalence, clinicohaemato-biochemical alterations in colibacillosis in neonatal calves. Int J Current Microbiol App Sci, 9, 3192-3198. 21. Bashir S., Ashraf I., Dar AM., Majid A., Bhat AA., Farooq J., 2015. Isolation and detection of Escherichia coli from diarrheic calves along with biochemical and hematologic parameters. J. Cell Tissue Res., 15,2,5059-61. 22. Malik S., Kumar A., Verma AK., Gupta MK., Sharma SD., Sharma AK., Rahal A., 2013.Haematological profile and blood chemistry in diarrhoeic calves affected with collibacillosis. J Anim Health Prod, 1, 10-14
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Araştırma Makaleleri |
| Authors | |
| Publication Date | April 30, 2020 |
| Published in Issue | Year 2020 Volume: 15 Issue: 1 |