Pathogenic Bacteria Present in the Lochia First 10−Day Postpartum Prolongs Days Open in Dairy Cows

Year 2021, Volume: 16 Issue: 1, 32 - 40, 26.04.2021

Mehmet Cemal Adıguzel , Seyda Cengiz , Mehmet Cengiz , Armağan Hayırlı

Abstract

The aims of this study were to evaluate the antibiotic resistance profiles and virulence genes of bacteria (Escherichia coli, Trueperella pyogenes, Prevotella melaninogenica, and Fusobacterium necrophorum) isolated from lochia during early postpartum and their effects on days open. The study was conducted on isolates, which were obtained from uterine discharges in the first 10 days of postpartum period, from 36 multiparous Simmental cows. The virulence genes of the isolates were investigated by polymerase chain reaction. The antimicrobial susceptibilities of the isolates were examined by the disc diffusion method. T. pyogenes (n = 97) and E. coli (n = 47) were revealed to carry the virulence gene (name of the gene), but not F. necrophorum (n = 16) and P. melaninogenica (n = 15). All isolates tested in this study were significantly sensitive to beta−lactam and tetracycline. E. coli and T. pyogenes were susceptible to aminoglycoside antibiotics, while F. necrophorum and P. melaninogenica were resistant to them. E. coli and F. necrophorum were susceptible to trimethoprim−sulfamethoxazole while T. pyogenes and P. melaninogenica were resistant. Those bacteria were predominant in cows with a prolonged days open (P<0.05). The uterine bacteria are responsible for prolonged days open and beta−lactam−derived antibiotics could be used as the first choice in the treatment of fresh cow uterine infections.

Keywords

Antimicrobial resistance, Bacteria, Cows, Days open, Uterus inflammation

Supporting Institution

Scientific Research Projects Coordination Unit of Atatürk University

Project Number

THD−2017−6207

Doğum Sonrası İlk On Günde Loşyada Bulunan Patojen Bakteriler Süt Sığırlarında Boş Gün Süresini Uzatır

Abstract

Bu çalışmanın amacı, postpartum erken dönemde loşyadan izole edilen bakterilerin (Escherichia coli, Trueperella pyogenes, Prevotella melaninogenica ve Fusobacterium necrophorum) antibiyotik direnç profili, virülans geni ve bunların ineklerde yeniden gebe kalma süresi üzerindeki etkilerini değerlendirmektir. Çalışma, birden fazla doğum yapmış 36 Simental inekten postpartum ilk 10 gündeki uterus akıntısından elde edilen izolatlar üzerinde gerçekleştirildi. Kültür ve biyokimyasal özellikleri belirlenen izolatların virülans genleri polimeraz zincir reaksiyonu ile incelendi. İzolatların antimikrobiyal duyarlılıkları disk difüzyon yöntemi ile saptandı. Çalışmadaki izole edilen T. pyogenes (n = 97) ve E. coli'nin (n = 47) virülans geni taşıdığı, ancak F. necrophorum (n = 16) ve P. melaninogenica (n = 15)’nın taşımadığı belirlendi. Tüm izolatlar beta − laktam antibiyotiklere ve tetrasikline karşı önemli derecede duyarlıydı. E. coli ve T. pyogenes, bu çalışmada aminoglikozid antibiyotiklere duyarlı iken F. necrophorum ve P. melaninogenica dirençliydi. E. coli ve F. necrophorum trimetoprim − sülfametoksazole duyarlı iken T. pyogenes ve P. melaninogenica dirençliydi. Ayrıca, E. coli, T. pyogenes, P. melaninogenica ve F. necrophorum’un yeniden gebe kalma süresi uzayan hayvanlarda öne çıkan bakteriler idi (P<0.05). Bu bulgular, uterus bakterilerinin yeniden gebe kalma süresinin uzamasına neden olduğunu ve uterus infeksiyonlarının tedavisinde ilk seçenek olarak beta − laktam türevi antibiyotiklerin kullanılabileceğini ortaya koymaktadır.

Keywords

Antimikrobiyal direnç, Bakteri, İnek, Uterus inflamasyonu, Yeniden gebe kalma süresi

Project Number

THD−2017−6207

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