Valproik Asit ve Cisplatin Kombinasyonunun Nöroblastom Tümörlerinde Canlılık Üzerine Antitümör Etkisinin İn Vitro Olarak Belirlenmesi

Year 2021, Volume: 16 Issue: 2, 182 - 188, 31.10.2021

Ali Taghizadehghalehjoughi , Sıdıka Genç , Yeşim Yeni , Ahmet Hacımüftüoğlu

https://doi.org/10.17094/ataunivbd.842103

Abstract

Nöroblastoma (SH-SY5Y), agresif ve dirençli özellikleri ile çocuklarda en sık görülen solid tümörlerden biridir. Valproik asit (VPA) bir histon deasetilaz inhibitörüdür (HDAC) ve antitümoral aktiviteye sahip olduğu düşünülmektedir. Bu çalışmanın amacı valproik asit ve sisplatin (CSP) kombinasyonun SH-SY5Y üzerindeki antitümör etkilerinin değerlendirilmesidir. Bu amaçla farklı dozlarda sisplatin (5, 10 ve 15 μg / ml), VPA (5mM) ve CSP (5, 10 ve 15 μg / ml) + VPA (5 mM) SH-SY5Y'ye hücre hattına 24 saat boyunca uygulanmıştır. Hücre canlılığı, apoptoz, antioksidan ve oksidan durumunun değerlendirilmesi için, 3- (4,5-Dimetiltiyazol-2-Yl) -2,5-Difeniltetrazolyum Bromür (MTT), Anexin-V-FITC apoptoz, Toplam Antioksidan Kapasite (TAC) ve Toplam Oksidan Durum (TOS) testleri ilaç uygulamasından 24 saat sonra yapılmıştır. Sonuçlarımıza göre, 15 μg / ml sisplatin ve 5 mM VPA kombinasyonu, negatif kontrol grubuna kıyasla hücre canlılığını azalttığı ve apoptoz durumunu arttırdığı tespit edilmiştir (P<0.05). Çalışmamızın sonuçları, valproik asidin cisplatin etkisini artırdığını ve kanser hücrelerinin canlılığını azalttığını ve VPA + CSP kombinasyonunun tek başına sispilatin uygulamasına kıyasla daha etkili olduğunu göstermektedir. Bu veriler ışığında VPA + CSP kombine tedavi etkinliği in vivo çalışmalar ile daha geniş kapsamlı araştırılması gerektiği düşünülmektedir.

Keywords

Annexin V, Cisplatin, Neuroblastoma, TAC, TOS

Supporting Institution

yoktur

Project Number

yoktur

In Vitro Determination of Valproic Acid and Cisplatin Combination Antitumor Effect on Neuroblastoma Tumors Viability

Abstract

Neuroblastoma (SH-SY5Y) is one of the most common solid tumors in children, with aggressive and resistant features. Valproic acid (VPA) is a histone deacetylase inhibitor (HDAC) and is thought to have antitumoral activity. The aim of the current study is the evaluation of valproic acid and cisplatin (CSP) combination antitumor effects on SH-SY5Y. For this aim, the different doses of cisplatin (5, 10, and 15 μg/ml), VPA (5mM), and CSP (5, 10 and 15 μg/ml) + VPA (5 mM) were applied on SH-SY5Y tumor cell culture for 24 hours. For evaluation of cell viability, apoptosis, antioxidant and oxidant status, 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT), Anexin-V-FITC apoptosis, Total Antioxidant Capacity (TAC) and Total Oxidant Status (TOS) tests were done 24 hours after drug administration. According to our results, the combination of 15 μg/ml cisplatin and 5 mM VPA reduced cell viability and increased apoptosis status, compared with the negative control group (P<0.05). Our studies showed valproic acid increased the cisplatin effect and reduced the viability of tumor cells. The combination of VPA + CSP is more effective than only using cisplatin. In the light of these data, it is thought that the efficacy of VPA + CSP combined therapy should be investigated more comprehensively with in vivo studies.

Keywords

Annexin V, Cisplatin, Neuroblastoma, TAC, TOS

Project Number

yoktur

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