Evaluation of the Relationship between eNOS and Breast Cancer

Abstract

Objective: The aim of this study was to determine the relationship between polymorphisms of endothelial nitric oxide synthase (eNOS) gen and development of breast cancer.

Material and methods: Thirty-seven cases with breast cancer and seventy healthy controls were enrolled in the present study. The study focused on three functional variants; a variant in a variable number of 27 bp tandem repeats in intron 4 (VNTR) of eNOS gene. We genotyped these variants using the polymerase chain reaction (PCR) and/or PCR-restriction fragment length polymorphism (RFLP) method. The distribution of allele and genotype in eNOS was compared between cases with breast cancer and healthy controls using chi-square test. 

Results: With regard to the eNOS (VNTR) variant, significantly decreased breast cancer risk was found for eNOS BB polymorphism (OR=0.56; 95%CI:0.463-0.676; p=0.001). No statistical association were found between the eNOS AA and AB polymorphisms and breast cancer risk (p=0.223 and 0.487).

Conclusion: In the current study, eNOS BB, was found to be associated with breast cancer. In contrast, eNOS AA genotype was not associated with breast cancer. Further studies are needed to determine whether these gene polymorphisms have a place in diagnosis or determining the risk of disease.

Keywords

• Breast cancer,Endothelial nitric oxide synthase,Risk factor

References

1. 1.Torre LA, Siegel RL, Ward EM et al. Global cancer ncidence and mortality rates and trends-an update. Cancer Epidemiol Biomark Prev 2016;25(1):16-27.
2. 2. Kelly KM, Shah N, Shedlosky-Shoemaker R et al. Living post treatment: Definitions of those with history and no history of cancer. J Cancer Surv 2011;5:158-166.
3. 3. Lichtenstein P, Holm NV, Verkasalo PK. Environmental and heritable factors in the causation of cancer. N Engl J Med 2000;343:78-85.
4. 4. Kehrer JP, KlotzLO. Free radicals and related reactive species as mediators of tissue injury and disease: implications for health. Crit Rev Toxicol 2015;45(9):765-798.
5. 5. Steenport M, Eom H, Uezu M et al. Association of polymorphisms in myeloperoxidase and catalase genes with precancerous changes in the gastric mucosa of patients at inner-cityhospitals in New York. Oncol Re. 2007;18(1):235-240.
6. 6. Dosenko VE, Zagoriy VY, Haytovich NV et al. Allelic polymorphism of endothelial NO synthase gene and its functional manifestations. Acta Biochim Pol 2006;53:299-302.
7. 7. Wang X, Axelsson J, Nordfors L et al. Changes in fat mass after initiation of maintenance dialysis is inflenced by the uncoupling protein 2 exon 8 insertion/deletion polymorphism. Nephrol Dial Transplant 2007;22:196-202.
8. 8. Gao X, Wang J, Wang W et al. eNOS Genetic Polymorphisms and Cancer Risk A Meta-Analysis and a Case–Control Study of Breast Cancer. Medicine (Baltimore) 2015;94(26): e972.

9. 9. Yao L, Fang F, Zhong Y et al. The association between two polymorphisms of eNOS and breast cancer risk:a meta-analysis. Breast Cancer Res Treat 2010;124:223-227.