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Açıklanamayan İnfertil Çiftlerde Erkek Faktörü: Sperm DNA Fragmantasyonu Değerlendirmesi

Year 2020, Volume: 3 Issue: 3, 109 - 113, 01.11.2020

Hale Bayram

Abstract

Açıklanamayan infertilite günümüzde tüm infertil çiftlerin % 30-40 ını oluşturmaktadır. Yapılan tüm infertilite tetkiklerinde sonuçların normal değerlendirilmesi sonucu daha ileri tetkikler yapılması gerekmektedir. Bu konuda en çok yapılan çalışma erkek partnerin sperm DNA bütünlüğünün değerlendirilmesidir. Sperm kalitesi gebelik oluşumu ve devamı için önemlidir. Sperm DNA bütünlüğünü değerlendirmek için TdT-dUTP nick-end-labelling (TUNEL) yöntemi, tek hücre jel elektroforezi (COMET analizi), sperm kromatin yapı analizi (SCSA), flow sitometrik analiz, akridin orange testi ve floresan in situ hibridizasyon (FISH) yöntemi sıklıkla kullanılmaktadır. Bazı çalışmalarda testlerden sadece biri kullanılırken bazılarında iki ya da daha fazlası kullanılmıştır. Yapılan çalışmalar açıklanamayan infertilite tanısı almış erkeklerde sperm DNA fragmantasyonu oranının yüksek olduğunu göstermiştir. Bu çiftlerde ya gebelik oluşmamakta ya da erken gebelik kayıpları görülmektedir.

Keywords

İnfertilite; erkek spermatozoa sperm DNA fragmantasyonu

References

  • 1. Speroff L, Glass RH, Kase NG. Infertility. In: Taylor HS, Pal L, Sell E, editors. Speroff's Clinical Gynecologic Endocrinology and Infertility içinde. 9th ed. Philadelphia: Lippincott Williams & Wilkins Press; 2019. p. 26-29.
  • 2. Satar DA, Gençdal S. Sperm Değerlendirmesi. Arşiv Kaynak Tarama Dergisi 2013; 22(4):532-42. doi: 10.17827/aktd.29343.
  • 3. Collins JA, Crosignani PG. Unexplained infertility: a review of diagnosis, prognosis, treatment efficacy and management. Int. J. Gynecol. Obstet. 1992;39(4):267-75. https://doi.org/10.1016/0020-7292(92)90257-J.
  • 4. Kan Ö, Alkılıç A, Yüce T, Berker B. Açıklanamayan İnfertilitede Yönetim. Arşiv Kaynak Tarama Dergisi. 2014; 23(3): 506-518. https://doi.org/10.17827/aktd.93154.
  • 5. Ferlin A, Raicu F, Gatta V, Zuccarello D, Palka G, Foresta C. Male infertility: role of genetic background. Reprod Biomed Online. 2007;14(6):734-45. https://doi.org/10.1016/S1472-6483(10)60677-3.
  • 6. Carrell DT, Liu L. Altered Protamine 2 Expression Is Uncommon In Donors of Known Fertility, but Common Among Men With Poor Fertilizing Capacity, and May Reflect Other Abnormalities of Spermiogenesis. J Androl. 2001;22(4):604-10.
  • 7. Saleh RA, Agarwal A, Nada EA, El-Tonsy MH, Sharma RK, Meyer A, vd. Negative effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility. Fertil Steril. 2003;79:1597-605.
  • 8. Shamsi MB, Imam SN, Dada R. Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertility. J Assist Reprod Genet. 2011;28(11):1073-85.
  • 9. Pastuszek E, Kiewisz J, Skowronska P, Liss J, Lukaszuk M, Bruszczynska A, vd. An investigation of the potential effect of sperm nuclear vacuoles in human spermatozoa on DNA fragmentation using a neutral and alkaline comet assay. Andrology. 2017;5(2):392-8.
  • 10. Evenson DP, Wixon R. Clinical aspects of sperm DNA fragmentation detection and male infertility. Theriogenology. 2006;65(5):979-91. doi: 10.1016/j.theriogenology.2005.09.011.
  • 11. Bareh GM, Jacoby E, Binkley P, Chang T “Arthur”, Schenken RS, Robinson RD. Sperm deoxyribonucleic acid fragmentation assessment in normozoospermic male partners of couples with unexplained recurrent pregnancy loss: a prospective study. Fertil Steril. 2016;105(2):329-36.e1. doi: 10.1016/j.fertnstert.2015.10.033.
  • 12. Küçük N. Sperm DNA and detection of DNA fragmentations in sperm. Turk J Urol. 2018;44(1):1-5.
  • 13. Singh NP, Muller CH, Berger RE. Effects of age on DNA double-strand breaks and apoptosis in human sperm. Fertil Steril. 2003;80(6):1420-30. doi: 10.1016/j.fertnstert.2003.04.002.
  • 14. O’Neill CL, Parrella A, Keating D, Cheung S, Rosenwaks Z, Palermo GD. A treatment algorithm for couples with unexplained infertility based on sperm chromatin assessment. J Assist Reprod Genet. 2018;35(10):1911-7. doi: 10.1007/s10815-018-1270-x.
  • 15. Feijó CM, Esteves SC. Diagnostic accuracy of sperm chromatin dispersion test to evaluate sperm deoxyribonucleic acid damage in men with unexplained infertility. Fertil Steril. 2014;101(1):58-63.e3. doi: 10.1016/j.fertnstert.2013.09.002.
  • 16. Hamada A, Esteves SC, Nizza M, Agarwal A. Unexplained Male infertility: diagnosis and Management. Int Braz J Urol. 2012;38(5):576-94. doi: 10.1590/s1677-55382012000500002.
  • 17. Robinson L, Gallos ID, Conner SJ, Rajkhowa M, Miller D, Lewis S, vd. The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta-analysis. Hum Reprod. 2012;27(10):2908-17.
  • 18. Agarwal A, Zini A, Sigman M. Is Sperm DNA Integrity Assessment Useful? Journal of Urology. 2013;190(5):1645-7. https://doi.org/10.1016/j.juro.2013.08.004
  • 19. Pons‐Rejraji H, Artonne C, Sion B, Brugnon F, Canis M, Janny L, vd. Prostasomes: inhibitors of capacitation and modulators of cellular signalling in human sperm. Int J Androl. 2011;34(6pt1):568-80. doi: 10.1111/j.1365-2605.2010.01116.x.

Year 2020, Volume: 3 Issue: 3, 109 - 113, 01.11.2020

Hale Bayram

Abstract

References

  • 1. Speroff L, Glass RH, Kase NG. Infertility. In: Taylor HS, Pal L, Sell E, editors. Speroff's Clinical Gynecologic Endocrinology and Infertility içinde. 9th ed. Philadelphia: Lippincott Williams & Wilkins Press; 2019. p. 26-29.
  • 2. Satar DA, Gençdal S. Sperm Değerlendirmesi. Arşiv Kaynak Tarama Dergisi 2013; 22(4):532-42. doi: 10.17827/aktd.29343.
  • 3. Collins JA, Crosignani PG. Unexplained infertility: a review of diagnosis, prognosis, treatment efficacy and management. Int. J. Gynecol. Obstet. 1992;39(4):267-75. https://doi.org/10.1016/0020-7292(92)90257-J.
  • 4. Kan Ö, Alkılıç A, Yüce T, Berker B. Açıklanamayan İnfertilitede Yönetim. Arşiv Kaynak Tarama Dergisi. 2014; 23(3): 506-518. https://doi.org/10.17827/aktd.93154.
  • 5. Ferlin A, Raicu F, Gatta V, Zuccarello D, Palka G, Foresta C. Male infertility: role of genetic background. Reprod Biomed Online. 2007;14(6):734-45. https://doi.org/10.1016/S1472-6483(10)60677-3.
  • 6. Carrell DT, Liu L. Altered Protamine 2 Expression Is Uncommon In Donors of Known Fertility, but Common Among Men With Poor Fertilizing Capacity, and May Reflect Other Abnormalities of Spermiogenesis. J Androl. 2001;22(4):604-10.
  • 7. Saleh RA, Agarwal A, Nada EA, El-Tonsy MH, Sharma RK, Meyer A, vd. Negative effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility. Fertil Steril. 2003;79:1597-605.
  • 8. Shamsi MB, Imam SN, Dada R. Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertility. J Assist Reprod Genet. 2011;28(11):1073-85.
  • 9. Pastuszek E, Kiewisz J, Skowronska P, Liss J, Lukaszuk M, Bruszczynska A, vd. An investigation of the potential effect of sperm nuclear vacuoles in human spermatozoa on DNA fragmentation using a neutral and alkaline comet assay. Andrology. 2017;5(2):392-8.
  • 10. Evenson DP, Wixon R. Clinical aspects of sperm DNA fragmentation detection and male infertility. Theriogenology. 2006;65(5):979-91. doi: 10.1016/j.theriogenology.2005.09.011.
  • 11. Bareh GM, Jacoby E, Binkley P, Chang T “Arthur”, Schenken RS, Robinson RD. Sperm deoxyribonucleic acid fragmentation assessment in normozoospermic male partners of couples with unexplained recurrent pregnancy loss: a prospective study. Fertil Steril. 2016;105(2):329-36.e1. doi: 10.1016/j.fertnstert.2015.10.033.
  • 12. Küçük N. Sperm DNA and detection of DNA fragmentations in sperm. Turk J Urol. 2018;44(1):1-5.
  • 13. Singh NP, Muller CH, Berger RE. Effects of age on DNA double-strand breaks and apoptosis in human sperm. Fertil Steril. 2003;80(6):1420-30. doi: 10.1016/j.fertnstert.2003.04.002.
  • 14. O’Neill CL, Parrella A, Keating D, Cheung S, Rosenwaks Z, Palermo GD. A treatment algorithm for couples with unexplained infertility based on sperm chromatin assessment. J Assist Reprod Genet. 2018;35(10):1911-7. doi: 10.1007/s10815-018-1270-x.
  • 15. Feijó CM, Esteves SC. Diagnostic accuracy of sperm chromatin dispersion test to evaluate sperm deoxyribonucleic acid damage in men with unexplained infertility. Fertil Steril. 2014;101(1):58-63.e3. doi: 10.1016/j.fertnstert.2013.09.002.
  • 16. Hamada A, Esteves SC, Nizza M, Agarwal A. Unexplained Male infertility: diagnosis and Management. Int Braz J Urol. 2012;38(5):576-94. doi: 10.1590/s1677-55382012000500002.
  • 17. Robinson L, Gallos ID, Conner SJ, Rajkhowa M, Miller D, Lewis S, vd. The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta-analysis. Hum Reprod. 2012;27(10):2908-17.
  • 18. Agarwal A, Zini A, Sigman M. Is Sperm DNA Integrity Assessment Useful? Journal of Urology. 2013;190(5):1645-7. https://doi.org/10.1016/j.juro.2013.08.004
  • 19. Pons‐Rejraji H, Artonne C, Sion B, Brugnon F, Canis M, Janny L, vd. Prostasomes: inhibitors of capacitation and modulators of cellular signalling in human sperm. Int J Androl. 2011;34(6pt1):568-80. doi: 10.1111/j.1365-2605.2010.01116.x.
There are 19 citations in total.

Details

Primary Language Turkish
Subjects Clinical Sciences
Journal Section Rewiev
Authors

Hale Bayram 0000-0002-6104-8820

Publication Date November 1, 2020
Acceptance Date August 19, 2020
Published in Issue Year 2020 Volume: 3 Issue: 3

Cite

APA Bayram, H. (2020). Açıklanamayan İnfertil Çiftlerde Erkek Faktörü: Sperm DNA Fragmantasyonu Değerlendirmesi. Tıp Fakültesi Klinikleri Dergisi, 3(3), 109-113.
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