PREPARATION OF A MODIFIED ELECTRODE FOR THE DETERMINATION OF LEVOFLOXACIN

Year 2017, Volume: 18 Issue: 5, 962 - 972, 31.12.2017

Ayça Atılır Özcan Mustafa Gürbüz Ali Özcan

https://doi.org/10.18038/aubtda.319445

Abstract

In
this study, modification of glassy carbon electrode with electrochemically
reduced graphene oxide (ERGO) and acid treated multi-walled carbon nanotubes
(A-MWCNT) was investigated for the voltammetric determination of LEV in body
fluids and pharmaceutical products. Different combinations of GO, ERGO, MWCNT
and A-MWCNT were searched and the best results obtained with GC/ERGO/A-MWCNT
electrode. The prepared modified electrode showed electrocatalytic acitivity
towards LEV oxidation by increasing oxidation peak currents 293 times.
Characterizations of the modified electrode was performed with cyclic
voltammetry, electrochemical impedance spectroscopy and raman measurements. The
highest oxidation peak current was observed at pH value of 2.0. The oxidation
peak currents of LEV increased linearly with the concentration of LEV in the
range of 0.01 mM and 10 mM. The
detection limit of the prepared sensor was determined as 0.0063 mM. After three weeks’ storage, the prepared electrode
showed high stability by preserving 93% of its original activity towards 1.0 mM LEV. The prepared electrode successfully
discriminates the voltammetric response of LEV from that of other
fluoroquinolone antibiotics (enoxacin, norfloxacin, ciprofloxacin). Finally,
the performance of the prepared electrode was tested in the determination of
LEV in human blood serum, urine and pharmaceutical samples.

Keywords

Levofloxacin, graphene oxide, multi-walled carbon nanotube, voltammetry, electrochemical sensor

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