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DECOLORIZATION OF DENIM DYESTUFF BY LACCASE ENZYME

Year 2011, Volume: 1 Issue: 1, 59 - 70, 09.02.2011

Abstract

Large quantities of dyes used in the textile industry are discharged to recipient environment during manufacture. This situation is beginning of a process which is difficult to recovery and relevant to environment and human health. Therefore, pollution of dyestuff produced textile industry will be reduced by cleaning of polluted area and integrating biological approaches with technologies having polluting potential. In scope of this study, commercial denim dye was decolorized by using high laccase activity culture supernatant of Trametes versicolor ATCC 200801 pellets grown in potato dextrose broth including wheat bran and determined optimum conditions. In the result of experiments done, pH, initial dye concentration, temperature and incubation time were selected 4.0, 75 mg/l, 55 oC and 120 minutes,  respectively. 68.02 % of decolorization was obtained at the  determined optimum conditions. Furthermore, adding different  metal ions to find in textile wastewater and supplementary
chemical materials used fabric dyeing process to reaction  medium, potential of decolorization copied with improvement was investigated effects of these. When the obtained data were examined, pollutants which tested at optimum conditions were observed not affected negatively decolorization. Even in the presence of Tween 80 detected the maximum inhibitor effect, 54.68 % of decolorization was obtained.

References

  • Abadulla, E., Tzanov, T., Costa, S., Robra, K.H., Cavaco-Paulo, A. ve Guebitz, G.M. (2000). Decolorization and detoxification of textile dyes with a laccase from Tram- etes hirsuta. Appl. Environ. Microbiol. 66, 3357–3362.
  • Aksu, Z. ve Çağatay, Ş.Ş. (2006). Investigation of biosorption of Gemazol Turquise Blue- G reactive dye by dried Rhizopus arrhizus in batch and continuous systems. Sep. Pu- rif. Technol. 48(1), 24-35.
  • Banat, I.M., Nigam, P., Singh, D. ve Marchant, R. (1996). Microbial decolorizatiın of tex- tile dye containing effluents:A review. Bioresource Technology 58, 217-227.
  • Cameselle, C., Pazos, M., Lorenzo, M. ve San- roman, M.A. (2003). Enhanced decolori- zation ability of laccase towards various synthetic dyes by an electrocatalysis tech- nology. Biotechnol. Lett. 25, 603–606.
  • Gedikli, S. (2008). Çeşitli Makrofungus İzolat- larının Lakkaz Üretim Yetenekleri Açıs- ından Değerlendirilmesi ve Dekolorizas- yon Uygulamalarında Kullanılabilirliği. Eskişehir Osmangazi Üniversitesi, Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, Eskişehir.
  • Gianfreda, L., Xu, F. ve Bollag, J. (1999). Lac- case: A useful Group of oxidoreductive enzymes. Biorem. J. 3, 1-25.
  • Kocaer, F.O. ve Alkan, U. (2002). boyarmadde içeren tekstil atıksularının arıtım alternati- fleri. Uludağ Üniversitesi Mühendislik- Mimarlık Fakültesi Dergisi 7(1), 47-55.
  • Kumar, K.V., Ramamurthi, V. ve Sivanesan, S. (2006). Biosorption of malachite green, a cationic dye onto Pithophora sp., a fresh water algae. Dyes and Pigments 69, 74- 79.
  • Kunamneni, A., Ghazi, I., Camarero, S., Balles- teros, A., Plou, F.J. ve Alcalde, M. (2008). Decolorization of synthetic dyes by laccase immobilized on epoxy- activated carriers. Process Biochemistry 43(2), 169-178.
  • Monteiro, M.C. ve Carvalho, M.E.A. (1998). Pulp bleaching using laccase from Tram- etes versicolor under high temperature and alkaline conditions. Appl. Biochem. Biotechnol. 70-72, 983-993.
  • Mougin, C., Jolivalt, C., Briozzo, P. ve Madzak, C. (2003). Fungal laccases: from struc- ture-activity studies to environmental ap- plications. Environmental Chemistry Let- ters 1(2), 145-148.
  • Nagai, M., Sato, T., Watanabe, H., Saito, K., Kawata, M. ve Enei, H., (2002). Purifica- tion and characterization of an extracellu- lar laccase from the edible mushroom Lentinula edodes and decolorization of chemically different dyes. Appl. Micro- biol. Biotechnol. 60, 327–335.
  • Nyanhongo, G.S., Gomes, J., Guebitz, G.M., Zvauya, R., Read, J. ve Steiner, W. (2002). Decolorization of textile dyes by laccases from a newly isolated strain of Trametes modesta. Water Research 36, 1449–1456.
  • Robinson, T., McMullan, G., Marchant, R. ve Nigam, P. (2001). Remediation of dyes in textile effluents: A critical review on cur- rent treatment technologies with a pro- posed alternative. Bioresource Technol- ogy 77, 247-255.
  • Rodríguez, C.S., Sanromán, M.A., Hofer, D. ve Gübitz, G.M. (2004). Production of lac- case by Trametes hirsuta grown in an immersion bioreactor and its application in the decolorization of dyes from a leather factory. Engineering in Life Sci- ence 4(3), 233-238.
  • Rodriguez, C.S., Sanroman, M. ve Gübitz, G.M. (2005). Influence of redox mediators and metal ions on synthetic acid dye decol- ourization by crude laccase from Tram- etes hirsuta. Chemosphere 58, 417–422.
  • Rodriguez, C.S. ve Herrara, F.L.T. (2006). In- dustrial and biotechologial applications of laccase. Biotechnology Advances 24, 500- 513.
  • Rodriguez, E., Pickard, M.A. ve Vazquez- Duhalt, R. (1999). Industrial dye decol- orization by laccases from ligninolytic fungi. Curr. Microbiol. 38, 27–32.
  • Saparrat, M.C.N., Mocchiutti, P., Liggieri, C.S., Aulicino, M.B., Caffini, N.O., Balatti, P.A. ve Martinez, M.J. (2007). Ligni- nolytic Enzyme ability And Potential Bio- technology Applications Of The White- rot Fungus Grammothele subargentea LPSC No: 436 Strain. Proces Biochemis- try 43, 368-375.
  • Selvam, K., Swaminathan, K. ve Chae, K.S. (2003). Decolorization of azo dyes and a dye industry effluent by a white rot fun- gus Thelephora sp. Bioresource Technol- ogy 88(2), 115–119.
  • Stolz, A. (2001). Basic and applied aspects in the microbial degradation of azo dyes. Appl. Microbiol Biotechnol. 56, 69-80.
  • Taşpınar, A. ve Kolankaya, N. (1998). Optimi- zation of enzymatic chlorine removal from Kraft pulp. Bull. of Environ. Con- tamin. & Toxicol. 61, 15-21.
  • Ünyayar, A., Mazmanci, M.A., Atacag, H., Erkurt, E.A. ve Coral, G. (2005). A Dri- maren Blue X3LR dye decolorizing en- zyme from Funalia trogii one step isola- tion and identification. Enzyme Microb. Technol. 36, 10–16.

LAKKAZ ENZİMİ İLE KOT BOYARMADDESİNİN DEKOLORİZASYONU

Year 2011, Volume: 1 Issue: 1, 59 - 70, 09.02.2011

Abstract

Tekstil endüstrisinde kullanılan çeşitli boyarmaddeler üretim sürecinde alıcı ortamlara büyük miktarlarda deşarj edilmektedir. Bu durum, çevre ve insan sağlığı ile ilgili telafisi zor olan bir sürecin başlangıcını oluşturmaktadır. Bu nedenle gerek kirlenmiş alanların temizlenmesinde ve gerekse kirletici potansiyeli olan teknolojilerin biyolojik yaklaşımlarla bütünleştirilmesi ile tekstil endüstrisinin bir atığı olan boyarmaddelerin oluşturacağı kirlilik azaltılabilecektir. Bu çalışma kapsamında ticari olarak piyasada satılan bir kot boyasının Trametes versicolor ATCC 200801’in buğday kepeği içeren potato dekstroz broth besiyerinde geliştirilmesi ile elde edilen lakkaz aktivitesi yüksek kültür sıvısı ile renk giderimi çalışılmış ve optimum koşullar belirlenmiştir. Yapılan deneyler sonucunda pH 4.0, başlangıç boya konsantrasyonu 75 mg/l, sıcaklık 55 oC, inkübasyon süresi ise 120 dakika olarak seçilmiştir. Belirlenen optimum koşullarda % 68.02 renk giderimi elde edilmiştir. Bununla birlikte, boya çözeltisi içerisine tekstil atık sularında bulunabilecek çeşitli metal iyonları ve kumaş boyama işleminde kullanılan yardımcı kimyasal maddeler reaksiyon ortamına ilave edilerek, geliştirilmeye çalışılan renk giderim potansiyeli üzerine olası etkileri araştırılmıştır. Elde edilen veriler değerlendirildiğinde, belirlenen optimum koşullarda denenen kirleticilerin renk giderimini genel olarak olumsuz yönde etkilemediği görülmektedir. En fazla inhibe edici etkinin görüldüğü 10 mM Tween 80 varlığında bile % 54.68 düzeyinde bir renk giderimi elde edilmiştir

References

  • Abadulla, E., Tzanov, T., Costa, S., Robra, K.H., Cavaco-Paulo, A. ve Guebitz, G.M. (2000). Decolorization and detoxification of textile dyes with a laccase from Tram- etes hirsuta. Appl. Environ. Microbiol. 66, 3357–3362.
  • Aksu, Z. ve Çağatay, Ş.Ş. (2006). Investigation of biosorption of Gemazol Turquise Blue- G reactive dye by dried Rhizopus arrhizus in batch and continuous systems. Sep. Pu- rif. Technol. 48(1), 24-35.
  • Banat, I.M., Nigam, P., Singh, D. ve Marchant, R. (1996). Microbial decolorizatiın of tex- tile dye containing effluents:A review. Bioresource Technology 58, 217-227.
  • Cameselle, C., Pazos, M., Lorenzo, M. ve San- roman, M.A. (2003). Enhanced decolori- zation ability of laccase towards various synthetic dyes by an electrocatalysis tech- nology. Biotechnol. Lett. 25, 603–606.
  • Gedikli, S. (2008). Çeşitli Makrofungus İzolat- larının Lakkaz Üretim Yetenekleri Açıs- ından Değerlendirilmesi ve Dekolorizas- yon Uygulamalarında Kullanılabilirliği. Eskişehir Osmangazi Üniversitesi, Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, Eskişehir.
  • Gianfreda, L., Xu, F. ve Bollag, J. (1999). Lac- case: A useful Group of oxidoreductive enzymes. Biorem. J. 3, 1-25.
  • Kocaer, F.O. ve Alkan, U. (2002). boyarmadde içeren tekstil atıksularının arıtım alternati- fleri. Uludağ Üniversitesi Mühendislik- Mimarlık Fakültesi Dergisi 7(1), 47-55.
  • Kumar, K.V., Ramamurthi, V. ve Sivanesan, S. (2006). Biosorption of malachite green, a cationic dye onto Pithophora sp., a fresh water algae. Dyes and Pigments 69, 74- 79.
  • Kunamneni, A., Ghazi, I., Camarero, S., Balles- teros, A., Plou, F.J. ve Alcalde, M. (2008). Decolorization of synthetic dyes by laccase immobilized on epoxy- activated carriers. Process Biochemistry 43(2), 169-178.
  • Monteiro, M.C. ve Carvalho, M.E.A. (1998). Pulp bleaching using laccase from Tram- etes versicolor under high temperature and alkaline conditions. Appl. Biochem. Biotechnol. 70-72, 983-993.
  • Mougin, C., Jolivalt, C., Briozzo, P. ve Madzak, C. (2003). Fungal laccases: from struc- ture-activity studies to environmental ap- plications. Environmental Chemistry Let- ters 1(2), 145-148.
  • Nagai, M., Sato, T., Watanabe, H., Saito, K., Kawata, M. ve Enei, H., (2002). Purifica- tion and characterization of an extracellu- lar laccase from the edible mushroom Lentinula edodes and decolorization of chemically different dyes. Appl. Micro- biol. Biotechnol. 60, 327–335.
  • Nyanhongo, G.S., Gomes, J., Guebitz, G.M., Zvauya, R., Read, J. ve Steiner, W. (2002). Decolorization of textile dyes by laccases from a newly isolated strain of Trametes modesta. Water Research 36, 1449–1456.
  • Robinson, T., McMullan, G., Marchant, R. ve Nigam, P. (2001). Remediation of dyes in textile effluents: A critical review on cur- rent treatment technologies with a pro- posed alternative. Bioresource Technol- ogy 77, 247-255.
  • Rodríguez, C.S., Sanromán, M.A., Hofer, D. ve Gübitz, G.M. (2004). Production of lac- case by Trametes hirsuta grown in an immersion bioreactor and its application in the decolorization of dyes from a leather factory. Engineering in Life Sci- ence 4(3), 233-238.
  • Rodriguez, C.S., Sanroman, M. ve Gübitz, G.M. (2005). Influence of redox mediators and metal ions on synthetic acid dye decol- ourization by crude laccase from Tram- etes hirsuta. Chemosphere 58, 417–422.
  • Rodriguez, C.S. ve Herrara, F.L.T. (2006). In- dustrial and biotechologial applications of laccase. Biotechnology Advances 24, 500- 513.
  • Rodriguez, E., Pickard, M.A. ve Vazquez- Duhalt, R. (1999). Industrial dye decol- orization by laccases from ligninolytic fungi. Curr. Microbiol. 38, 27–32.
  • Saparrat, M.C.N., Mocchiutti, P., Liggieri, C.S., Aulicino, M.B., Caffini, N.O., Balatti, P.A. ve Martinez, M.J. (2007). Ligni- nolytic Enzyme ability And Potential Bio- technology Applications Of The White- rot Fungus Grammothele subargentea LPSC No: 436 Strain. Proces Biochemis- try 43, 368-375.
  • Selvam, K., Swaminathan, K. ve Chae, K.S. (2003). Decolorization of azo dyes and a dye industry effluent by a white rot fun- gus Thelephora sp. Bioresource Technol- ogy 88(2), 115–119.
  • Stolz, A. (2001). Basic and applied aspects in the microbial degradation of azo dyes. Appl. Microbiol Biotechnol. 56, 69-80.
  • Taşpınar, A. ve Kolankaya, N. (1998). Optimi- zation of enzymatic chlorine removal from Kraft pulp. Bull. of Environ. Con- tamin. & Toxicol. 61, 15-21.
  • Ünyayar, A., Mazmanci, M.A., Atacag, H., Erkurt, E.A. ve Coral, G. (2005). A Dri- maren Blue X3LR dye decolorizing en- zyme from Funalia trogii one step isola- tion and identification. Enzyme Microb. Technol. 36, 10–16.
There are 23 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Ahmet Çabuk

Serap Gedikli

Pınar Aytar

Arzu Ünal

Nazif Kolankaya This is me

Publication Date February 9, 2011
Published in Issue Year 2011 Volume: 1 Issue: 1

Cite

APA Çabuk, A., Gedikli, S., Aytar, P., Ünal, A., et al. (2011). DECOLORIZATION OF DENIM DYESTUFF BY LACCASE ENZYME. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, 1(1), 59-70.
AMA Çabuk A, Gedikli S, Aytar P, Ünal A, Kolankaya N. DECOLORIZATION OF DENIM DYESTUFF BY LACCASE ENZYME. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. April 2011;1(1):59-70.
Chicago Çabuk, Ahmet, Serap Gedikli, Pınar Aytar, Arzu Ünal, and Nazif Kolankaya. “DECOLORIZATION OF DENIM DYESTUFF BY LACCASE ENZYME”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 1, no. 1 (April 2011): 59-70.
EndNote Çabuk A, Gedikli S, Aytar P, Ünal A, Kolankaya N (April 1, 2011) DECOLORIZATION OF DENIM DYESTUFF BY LACCASE ENZYME. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 1 1 59–70.
IEEE A. Çabuk, S. Gedikli, P. Aytar, A. Ünal, and N. Kolankaya, “DECOLORIZATION OF DENIM DYESTUFF BY LACCASE ENZYME”, Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, vol. 1, no. 1, pp. 59–70, 2011.
ISNAD Çabuk, Ahmet et al. “DECOLORIZATION OF DENIM DYESTUFF BY LACCASE ENZYME”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 1/1 (April 2011), 59-70.
JAMA Çabuk A, Gedikli S, Aytar P, Ünal A, Kolankaya N. DECOLORIZATION OF DENIM DYESTUFF BY LACCASE ENZYME. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. 2011;1:59–70.
MLA Çabuk, Ahmet et al. “DECOLORIZATION OF DENIM DYESTUFF BY LACCASE ENZYME”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, vol. 1, no. 1, 2011, pp. 59-70.
Vancouver Çabuk A, Gedikli S, Aytar P, Ünal A, Kolankaya N. DECOLORIZATION OF DENIM DYESTUFF BY LACCASE ENZYME. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. 2011;1(1):59-70.