GPSE (GRAPE SEED PROANTHOCYANIDIN EXTRACT) SIMVASTATIN AND SILYMARIN EFFECT ON HIGH CHOLESTEROL-FED ANIMALS

Year 2012, Volume: 2 Issue: 2, 101 - 117, 30.01.2012

Dilek Burukoğlu , Emine Sunal Fatma Kılıç , Sema Uslu , Setenay Öner , Ayla Eker Sarıboyacı Dilek Burukoğlu , Murat Kılıçoğlu

Abstract

The present study was designed to test the hypothesis that cholesterol rich diet-induced hypercholesterolemia in rabbits can be reversed by long-term treatment of antioxidants (proanthocyanidin and silymarin) and antihyperlipidemic drug(simvastatin). In this study 35 adult New Zealand White (NZW) male rabbits were used and divided into five groups. The rabbits in Group 1(control) was fed with rabbit chow diet. Group 2 was fed with only cholesterol (2% w/w) rich diet to induce hypercholesterolemia. The other groups received GPSE 100mg/kg (Group 3), silymarin 10mg/kg (Group 4) and simvastatin10mg/kg (Group 5) with cholesterol in addition to rabbit chow diet. At the end of the experiment, the animals were sacrified and their aorta were excised for intimal lesion analysis. Vessells were dissected, trimmed, and then placed fixed in neutral formalin. All tissue sections were stained with hematoxylin and eosin(H&E). Apoptotic process was detected by using TUNEL.

Cholesterol treatment produced a sustainable state of atheromatous plaque formation In addition, only cholesterol rich diet-induced animals have shown a considerably high number apoptotic cells by using TUNEL and plasma total cholesterol (TC) and low density lipoprotein (LDL) levels were found.

Keywords

Atherosclerosis; Grape Seed Extract; Proanthocyanidin; Simvastatin; Silymarin

Yaşam Bilimleri ve Biyoteknoloji

Abstract

Araştırmamızda kolesterolden zengin diyet ile beslenen tavşanlarda hiperkolesterolemi üzerine uzun süreli antioksidanların (üzüm çekirdeği ekstresi ve silymarin) ve antihiperlipidemik ilaçların (simvastatin) kullanımının etkisi incelenmiştir

Keywords

Ateroskleroz, Üzüm çekirdeği ekstresi, Simvastatin, Silymarin

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