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EFFECT OF EXOGENOUSLY APPLIED SPERMINE AND PUTRESCINE ON GERMINATION AND IN VITRO GROWTH OF PEPPER (CAPSICUM ANNUUM L.) SEEDS UNDER SALT STRESS

Year 2014, Volume: 3 Issue: 2, 63 - 71, 05.05.2015
https://doi.org/10.18036/btdc.61431

Abstract

Plant growth regulators play a significant role in germination of seed. In this study,  Effects of exogenous polyamines (spermine and putrescine) on germination of seed and seedling growth such as radicula length, hypocotile length, fresh weight, dry weight of pepper under salt stress were investigated. According to results, increased salt concentration resulted in a significant reduction in germination (P<0.05). NaCl prevented hypocotile growth and this inhibition was reversed by applying spermine. During application of 2 mM spermine + 200 mM NaCl (P<0.05),  the best germination was recorded. On the contrary, germination of seed was inhibited by single application of Put but hypocotile and radicula length of seedling were increased seriously by application of 0.01 mM putrescine + 200mM NaCl and 1mM putrescine + 50 mM NaCl (P<0.05) in pepper plant.

 

References

  • Abdoli, M., Saeidi, M., Azhand, M., Honarmand, A.J., Esfandiari, E. and Shekari, F. (2013). The Effects of Different Levels of Salinity and Indole-3-Acetic Acid (IAA) on Early Growth and Germination of Wheat Seedling. Journal of Stress Physiology and Biochemistry 9 (4), 329-338.
  • Applewhite, P.B., Kaur-Sawhney, R. and Galston, A.W. (2000). A Role for Spermidine in The Bolting and Flowering of Arabidopsis. Physiologia Plantarum 108, 314-20.
  • Bagni, N. (1970). Metabolic Changes of Polyamines During The of Germination Phaseolus Vulgaris. New Phytologist 69, 159-164.
  • Barendse, G.W. and Peeters, T.J.M. (1995). Multiple Hormonal Control in Plants. Acta Botanica Neerlandica 44, 3-17.
  • Bewley, J.D. (1997). Seed Germination and Dormancy. The Plant Cell 9, 1055-1066.
  • Bohnert, H., Nelson, D.E. and Jensen, R.G. (1995). Adaptations to Environment Stresses. Plant Science 7, 1099-1111.
  • Bouchereau, A., Aziz, A., Larher, F. and Martin- Tanguy, J. (1999). Polyamines and Environmental Development. Plant Science 140, 103-25. Recent of for Society Horticultural
  • Farooq, M., Aziz, T., Rehman, H., Rehman, A., Cheema, S.A. and Aziz, T. (2011). Evaluating Surface Drying and Re-drying for Wheat Seed Priming with Polyamines: Effects on Emergence, Early Seedling Growth and Starch Metabolism. Acta Physiologia Plantarum 33,1707-1713.
  • Galston, A.W., Kaur-Sawhney, R., Altabella, T. and Tiburcio, Polyamines in Reproductive Activity and Response to Abiotic Stress. Botanica Acta 110, 197-207. (1997). Plant
  • Groppa, M.D., Zawoznik, M.S., Tomaro, M.L. and Benavides, M.P. (2007). Inhibition of Root Growth and Polyamine Metabolism in Seedlings under Cadmium and Copper Stress. Biological Trace Element Research 126, 1-3. annuus)
  • Huang, J. and Reddman, R.E. (1995). Salt Tolerance of Hordeum and Brassica Species During Germination and Early Seedling Growth. Canadian Journal of Plant Science 75, 815-819.
  • Igarashi, K. and Kashiwagi, K. (2000). Polyamines: Mysterious Modulators of Cellular Functions. Biochemical and Biophysical Research Communications 271, 559-564.
  • Jeannette, S., Jimenez, B., Craig, R. and Lynch, J.P. (2002). Phaseolus Species During Germination and Early Seedling Growth. Crop Science 42, 1584-1594. Tolerance of
  • Keiffer, C.H. and Ungar, I.A. (1997). The Effect of Extend Exposure to Hyper Saline Conditions on The Germination of Five Inland Halophyte Species. American Journal of Botany 84, 104-111.
  • Khan, M.I., Khan M.A. and Khizar T. (1976). Plant Growth Regulators from Species Differing in Salt Tolerance as Affected By Soil Salinity. Plant and Soil 45, 267-271.
  • Khan, H.Z., Ziaf, K., Amjad, M. and Iqbal, Q. (2012). Polyamines Improves Germination and Early Seedling Growth of Hot Pepper. Chilean Journal of Agricultural Research 72 (3), 429-433. Application of
  • Kusano, T., Berberich, T., Tateda, C. and Takahashi, Essential Factors for Growth and Survival. Planta 228, 367-381. Polyamines: Kuznetsov, V., Radyukina, Shevyakova, N.I. (2006). Polyamines and Stress: Biological Role, Metabolism and Regulation. Russian Journal of Plant Physiology 53, 583-604. N.L. and
  • Mass, E.V. and Poss, J.A. (1989). Salt Sensitivity of Wheat at Various Growth Stages. Irrigation Science 10, 2940.
  • Pieruzzi, F.P., Leonardo, L.C.D. , Tiago, S.B. , Santa-Catarina, C., André, L.W.D. and Floh, E.I.S. (2011). Polyamines, IAA and ABA During Germination in Two Recalcitrant Seeds: Araucaria Angustifolia (Gymnosperm) and Ocotea Odorifera (Angiosperm). Annals of Botany 108 (2), 337-345.
  • Saboury, A.A. and Karbassi, F. (2000). Thermodynamic Studies on The Intraction of Calcium Ion with Alpha-amylase. Thermochemical Acta 362, 121-129.
  • Santa-Catarina, C., Silveira, V., Balbuena, T.S., Maranha˜o, M.E.E., Handro, W. and Floh, E.I.S. (2006). IAA, ABA, Polyamines and Free Amino Acids Associated with Zygotic Embryo Development of Ocotea Catharinensis. Plant Growth Regulation 49, 237-247.
  • Shoeb, F., Yadav, J.S., Bajaj, S. and Rajam, M.V. (2001). Polyamines as Biomarkers for Plant Improvement Modulation of Polyamine Metabolism Indifferent Genotypes of Indica Rice. Plant Science 160, 1229-1235. Capacity: Regeneration By
  • Silveira, V., Santa-Catarina, C., Tun, N.N., Scherer, G.F.E., Handro, W., Guerra, M.P. and Floh, E.I.S. (2006). Polyamine Effects on The Endogenous Polyamine Contents, Nitric Oxide Release, Growth and Differentiation Suspension Angustifolia (Bert.) O. Ktze. Plant Science 171, 91-98. Embryogenic Cultures of Araucaria
  • Smith, T.A. (1982). The Function and Metabolism of Polyamines in Higher Plants. In: Plant Growth Substances, Ed: P.F. Wareing. 683. Academic Press, New York.
  • Strand, A.E., Pritchard, S.G., McCormack, M.L., Davis, M.A. and Oren, R. (2008). Irreconcilable Differences: Fine-Root Life Spans and Soil Carbon Persistence. Science 319, 456–458.
  • Takahashi, T., Kakehi, J.I. (2010). Polyamines: Ubiquitous Polycations with Unique Roles in Growth and Stress Responses. Annals of Botany 105, 1-6.
  • Tassoni, A., Van Buuren, M., Franceschetti, M., Fornale, S. and Bagni, N. (2000). Polyamine Content and Metabolism in Arabidopsis Thaliana and Effect of Spermidine on Plant Developmlent. Plant Physiology and Biochemistry 38, 383-93.
  • Ungar, I.A. (1991). Ecophysiology of Vascular Halophytes. Boca Raton, CRC Press, Florida.
  • Wahed, A. (2006). Exogenous and Endogenous Polyamines Relation to Growth, A- Cellulose Precipitation in Fibres and Productivity in Cotton Plant. Agricultural Science 2, 139-148.

ANADOLU ÜNİVERSİTESİ

Year 2014, Volume: 3 Issue: 2, 63 - 71, 05.05.2015
https://doi.org/10.18036/btdc.61431

Abstract

Bitki büyüme düzenleyicileri tohum çimlenmesinde önemli bir rol oynar. Bu çalışmada tuz stresi altında biberin fide büyümesi (radikula uzunluğu, hipkotil uzunluğu, taze ağırlık, kuru ağırlık) ve tohum çimlenmesi üzerine dışsal poliaminler (spermin ve putresin)’in etkisi araştırılmıştır. Artan tuz konsantrasyonu çimlenme üzerinde önemli bir azalmaya neden olmuştur (P<0.05). NaCl hipokotil büyümesini inhibe etmiştir ve bu inhibisyon spermin uygulaması ile engellenmiştir. En iyi çimlenme 2 mM spermin + 200 mM NaCl uygulamasında belirlenmiştir (P<0.05). Tek başına Put uygulması çimlenmeyi inhibe etmiştir, fakat hipokotil ve radikula uzunluğu 0.01 mM putresin + 200mM NaCl and 1mM putresin + 50 mM NaCl uygulamaları ile önemli bir şekilde artmıştır (P<0.05)

References

  • Abdoli, M., Saeidi, M., Azhand, M., Honarmand, A.J., Esfandiari, E. and Shekari, F. (2013). The Effects of Different Levels of Salinity and Indole-3-Acetic Acid (IAA) on Early Growth and Germination of Wheat Seedling. Journal of Stress Physiology and Biochemistry 9 (4), 329-338.
  • Applewhite, P.B., Kaur-Sawhney, R. and Galston, A.W. (2000). A Role for Spermidine in The Bolting and Flowering of Arabidopsis. Physiologia Plantarum 108, 314-20.
  • Bagni, N. (1970). Metabolic Changes of Polyamines During The of Germination Phaseolus Vulgaris. New Phytologist 69, 159-164.
  • Barendse, G.W. and Peeters, T.J.M. (1995). Multiple Hormonal Control in Plants. Acta Botanica Neerlandica 44, 3-17.
  • Bewley, J.D. (1997). Seed Germination and Dormancy. The Plant Cell 9, 1055-1066.
  • Bohnert, H., Nelson, D.E. and Jensen, R.G. (1995). Adaptations to Environment Stresses. Plant Science 7, 1099-1111.
  • Bouchereau, A., Aziz, A., Larher, F. and Martin- Tanguy, J. (1999). Polyamines and Environmental Development. Plant Science 140, 103-25. Recent of for Society Horticultural
  • Farooq, M., Aziz, T., Rehman, H., Rehman, A., Cheema, S.A. and Aziz, T. (2011). Evaluating Surface Drying and Re-drying for Wheat Seed Priming with Polyamines: Effects on Emergence, Early Seedling Growth and Starch Metabolism. Acta Physiologia Plantarum 33,1707-1713.
  • Galston, A.W., Kaur-Sawhney, R., Altabella, T. and Tiburcio, Polyamines in Reproductive Activity and Response to Abiotic Stress. Botanica Acta 110, 197-207. (1997). Plant
  • Groppa, M.D., Zawoznik, M.S., Tomaro, M.L. and Benavides, M.P. (2007). Inhibition of Root Growth and Polyamine Metabolism in Seedlings under Cadmium and Copper Stress. Biological Trace Element Research 126, 1-3. annuus)
  • Huang, J. and Reddman, R.E. (1995). Salt Tolerance of Hordeum and Brassica Species During Germination and Early Seedling Growth. Canadian Journal of Plant Science 75, 815-819.
  • Igarashi, K. and Kashiwagi, K. (2000). Polyamines: Mysterious Modulators of Cellular Functions. Biochemical and Biophysical Research Communications 271, 559-564.
  • Jeannette, S., Jimenez, B., Craig, R. and Lynch, J.P. (2002). Phaseolus Species During Germination and Early Seedling Growth. Crop Science 42, 1584-1594. Tolerance of
  • Keiffer, C.H. and Ungar, I.A. (1997). The Effect of Extend Exposure to Hyper Saline Conditions on The Germination of Five Inland Halophyte Species. American Journal of Botany 84, 104-111.
  • Khan, M.I., Khan M.A. and Khizar T. (1976). Plant Growth Regulators from Species Differing in Salt Tolerance as Affected By Soil Salinity. Plant and Soil 45, 267-271.
  • Khan, H.Z., Ziaf, K., Amjad, M. and Iqbal, Q. (2012). Polyamines Improves Germination and Early Seedling Growth of Hot Pepper. Chilean Journal of Agricultural Research 72 (3), 429-433. Application of
  • Kusano, T., Berberich, T., Tateda, C. and Takahashi, Essential Factors for Growth and Survival. Planta 228, 367-381. Polyamines: Kuznetsov, V., Radyukina, Shevyakova, N.I. (2006). Polyamines and Stress: Biological Role, Metabolism and Regulation. Russian Journal of Plant Physiology 53, 583-604. N.L. and
  • Mass, E.V. and Poss, J.A. (1989). Salt Sensitivity of Wheat at Various Growth Stages. Irrigation Science 10, 2940.
  • Pieruzzi, F.P., Leonardo, L.C.D. , Tiago, S.B. , Santa-Catarina, C., André, L.W.D. and Floh, E.I.S. (2011). Polyamines, IAA and ABA During Germination in Two Recalcitrant Seeds: Araucaria Angustifolia (Gymnosperm) and Ocotea Odorifera (Angiosperm). Annals of Botany 108 (2), 337-345.
  • Saboury, A.A. and Karbassi, F. (2000). Thermodynamic Studies on The Intraction of Calcium Ion with Alpha-amylase. Thermochemical Acta 362, 121-129.
  • Santa-Catarina, C., Silveira, V., Balbuena, T.S., Maranha˜o, M.E.E., Handro, W. and Floh, E.I.S. (2006). IAA, ABA, Polyamines and Free Amino Acids Associated with Zygotic Embryo Development of Ocotea Catharinensis. Plant Growth Regulation 49, 237-247.
  • Shoeb, F., Yadav, J.S., Bajaj, S. and Rajam, M.V. (2001). Polyamines as Biomarkers for Plant Improvement Modulation of Polyamine Metabolism Indifferent Genotypes of Indica Rice. Plant Science 160, 1229-1235. Capacity: Regeneration By
  • Silveira, V., Santa-Catarina, C., Tun, N.N., Scherer, G.F.E., Handro, W., Guerra, M.P. and Floh, E.I.S. (2006). Polyamine Effects on The Endogenous Polyamine Contents, Nitric Oxide Release, Growth and Differentiation Suspension Angustifolia (Bert.) O. Ktze. Plant Science 171, 91-98. Embryogenic Cultures of Araucaria
  • Smith, T.A. (1982). The Function and Metabolism of Polyamines in Higher Plants. In: Plant Growth Substances, Ed: P.F. Wareing. 683. Academic Press, New York.
  • Strand, A.E., Pritchard, S.G., McCormack, M.L., Davis, M.A. and Oren, R. (2008). Irreconcilable Differences: Fine-Root Life Spans and Soil Carbon Persistence. Science 319, 456–458.
  • Takahashi, T., Kakehi, J.I. (2010). Polyamines: Ubiquitous Polycations with Unique Roles in Growth and Stress Responses. Annals of Botany 105, 1-6.
  • Tassoni, A., Van Buuren, M., Franceschetti, M., Fornale, S. and Bagni, N. (2000). Polyamine Content and Metabolism in Arabidopsis Thaliana and Effect of Spermidine on Plant Developmlent. Plant Physiology and Biochemistry 38, 383-93.
  • Ungar, I.A. (1991). Ecophysiology of Vascular Halophytes. Boca Raton, CRC Press, Florida.
  • Wahed, A. (2006). Exogenous and Endogenous Polyamines Relation to Growth, A- Cellulose Precipitation in Fibres and Productivity in Cotton Plant. Agricultural Science 2, 139-148.
There are 29 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Esra Koç

Ayşen Üstün

Cemil İşlek This is me

Yeliz Kaşko Arıcı

Publication Date May 5, 2015
Published in Issue Year 2014 Volume: 3 Issue: 2

Cite

APA Koç, E., Üstün, A., İşlek, C., Kaşko Arıcı, Y. (2015). EFFECT OF EXOGENOUSLY APPLIED SPERMINE AND PUTRESCINE ON GERMINATION AND IN VITRO GROWTH OF PEPPER (CAPSICUM ANNUUM L.) SEEDS UNDER SALT STRESS. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, 3(2), 63-71. https://doi.org/10.18036/btdc.61431
AMA Koç E, Üstün A, İşlek C, Kaşko Arıcı Y. EFFECT OF EXOGENOUSLY APPLIED SPERMINE AND PUTRESCINE ON GERMINATION AND IN VITRO GROWTH OF PEPPER (CAPSICUM ANNUUM L.) SEEDS UNDER SALT STRESS. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. May 2015;3(2):63-71. doi:10.18036/btdc.61431
Chicago Koç, Esra, Ayşen Üstün, Cemil İşlek, and Yeliz Kaşko Arıcı. “EFFECT OF EXOGENOUSLY APPLIED SPERMINE AND PUTRESCINE ON GERMINATION AND IN VITRO GROWTH OF PEPPER (CAPSICUM ANNUUM L.) SEEDS UNDER SALT STRESS”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 3, no. 2 (May 2015): 63-71. https://doi.org/10.18036/btdc.61431.
EndNote Koç E, Üstün A, İşlek C, Kaşko Arıcı Y (May 1, 2015) EFFECT OF EXOGENOUSLY APPLIED SPERMINE AND PUTRESCINE ON GERMINATION AND IN VITRO GROWTH OF PEPPER (CAPSICUM ANNUUM L.) SEEDS UNDER SALT STRESS. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 3 2 63–71.
IEEE E. Koç, A. Üstün, C. İşlek, and Y. Kaşko Arıcı, “EFFECT OF EXOGENOUSLY APPLIED SPERMINE AND PUTRESCINE ON GERMINATION AND IN VITRO GROWTH OF PEPPER (CAPSICUM ANNUUM L.) SEEDS UNDER SALT STRESS”, Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, vol. 3, no. 2, pp. 63–71, 2015, doi: 10.18036/btdc.61431.
ISNAD Koç, Esra et al. “EFFECT OF EXOGENOUSLY APPLIED SPERMINE AND PUTRESCINE ON GERMINATION AND IN VITRO GROWTH OF PEPPER (CAPSICUM ANNUUM L.) SEEDS UNDER SALT STRESS”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 3/2 (May 2015), 63-71. https://doi.org/10.18036/btdc.61431.
JAMA Koç E, Üstün A, İşlek C, Kaşko Arıcı Y. EFFECT OF EXOGENOUSLY APPLIED SPERMINE AND PUTRESCINE ON GERMINATION AND IN VITRO GROWTH OF PEPPER (CAPSICUM ANNUUM L.) SEEDS UNDER SALT STRESS. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. 2015;3:63–71.
MLA Koç, Esra et al. “EFFECT OF EXOGENOUSLY APPLIED SPERMINE AND PUTRESCINE ON GERMINATION AND IN VITRO GROWTH OF PEPPER (CAPSICUM ANNUUM L.) SEEDS UNDER SALT STRESS”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, vol. 3, no. 2, 2015, pp. 63-71, doi:10.18036/btdc.61431.
Vancouver Koç E, Üstün A, İşlek C, Kaşko Arıcı Y. EFFECT OF EXOGENOUSLY APPLIED SPERMINE AND PUTRESCINE ON GERMINATION AND IN VITRO GROWTH OF PEPPER (CAPSICUM ANNUUM L.) SEEDS UNDER SALT STRESS. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. 2015;3(2):63-71.