ANTIOXIDATIVE RESPONSES IN Tubifex tubifex AGAINST THALLIUM INDUCED OXIDATIVE STRESS

Year 2017, Volume: 6 Issue: 2, 75 - 85, 31.08.2017

Gözde Aydoğan Kılıç , VOLKAN Kılıç

https://doi.org/10.18036/aubtdc.288885

Cited By: 1

Abstract

Thallium (Tl) impact on
living systems has become an important issue in recent years. However very few
data exist about the consequences of Tl exposure in freshwater aquatic systems.
The aim of the present study is to investigate alterations in the levels of antioxidative
biomarkers (metallothionein (MT), total glutathione (GSH), glutathione
peroxidase (GPx), glutathione S-transferase (GST) and catalase (CAT)) in the aquatic oligochaete Tubifex tubifex, in response to sublethal doses of Tl.  Experimental groups were
exposed to thallium acetate at 0.25, 0.5 and 1 µg l^-1 concentrations
during 7 or 15 days. MT
levels were observed to be interestingly decreased. 0.25 µg
l^-1 concentration had no effect on total GSH, GPx and GST activities
while 0.5 and 1 µg l^-1 concentrations induced total GSH and GPx
activitiy after 7 days. Longer exposure time resulted in a decrease in total
GSH along with GPx and GST activities at all concentrations of Tl. Despite the
significant elevation of CAT activity in all experimental groups, lipid
peroxidation (LP) was observed to be induced depending on the increasing
exposure concentration and time. The results suggested the possible mechanism
of action in the antioxidant system of T.
tubifex in response to Tl induced oxidative stress (OS) and provide useful
information for the future evaluation of Tl impact on aquatic ecosystems.

Keywords

Thallium, Tubifex tubfex, Oxidative stres, Antioxidants, Lipid peroxidation

Tubifex tubifex'DE TALYUM İLE İNDÜKLENEN OKSİDATİF STRESE KARŞI OLUŞAN ANTİOKSİDAN YANITLAR

Abstract

Talyum (Tl)’un canlı sistemler üzerindeki etkisi
son yıllarda önemli bir sorun haline gelmiştir. Bununla birlikte, sucul
sistemlerin Tl’a maruz kalmasının sonuçları ile ilgili çok az bilgi mevcuttur. Bu
çalışmanın amacı, sucul oligoket Tubifex
tubifex’in antioksidan biyolojik belirteçlerinde (metallotionein (MT), toplam glutatyon (GSH), glutatyon
peroksidaz (GPx), glutatyon S-transferaz (GST) ve katalaz (CAT)), TI’un
subletal dozlarına yanıt olarak meydana gelen değişikliklerin araştırılmasıdır.
Deney grupları 0.25, 0.5 ve 1 µg l^-1’lik konsantrasyonlarda, 7 ve 15
günlük süreler boyunca talyum asetat’a maruz bırakılmıştır. MT seviyelerinde
ilgi çekici bir azalma gözlenmiştir. 0.25 µg l^-1’ lık konsantrasyon
toplam GSH, GPx ve GST aktiviteleri üzerinde etki göstermezken, 0.5 ve 1 µg l^-1’
lık konsantrasyonlar toplam GSH ve GPx aktivitelerini 7 günün sonunda
indüklemiştir. Daha uzun maruz kalma süresi GPx ve GST aktiviteleri ile
birlikte toplam GSH’un da tüm konsantrasyonlarda azalmasına neden olmuştur. CAT
aktivitesinde tüm deney gruplarında gözlenen anlamlı artışa rağmen, lipid
peroksidasyonunun (LP) artan konsantrasyon ve süreye bağlı olarak arttığı
gözlenmiştir. Sonuçlar Tl ile indüklenen oksidatif strese karşı, T. tubifex’in antioksidan sisteminin olası
hareket mekanizmasını ortaya koymakta ve Tl’un sucul ekosistemlerdeki etkileri
ile ilgili gelecekte yapılacak değerlendirmeler için önemli bir bilgi kaynağı oluşturmaktadır.

Keywords

Talyum, Tubifex tubifex, Oksidatif stres, Antioksidanlar, Lipit peroksidasyonu

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