Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids

Year 2018, Volume: 7 Issue:1, 52 - 62, 28.02.2018

Yasemin Kemeç Hürkan Kaan Hürkan Cüneyt Akı

https://doi.org/10.18036/aubtdc.331328

Cited By: 1

Abstract

Due to the
increasing demand and over collection of orchids from nature to produce salep,
scientists have been led to search for more efficient ways to propagate these
specific orchids in vitro. This present study compares germination
performances of two commercial (Orchimax and Knudson C) and one specially
prepared orchid growth media (SV), on economically and medicinally important
orchids used to make salep; (Anacamptis pyramidalis, Dactylorhiza
romana, Neotinea tridentata and Anacamptis morio subsp.
morio) and further aims to obtain a mature orchid plant by following the
natural environmental cycle.

Significant
differences in seed germination and protocorm development were observed.
Asymbiotic germination tests showed that the specially prepared growth media
performed better than the commercial media by 79.11% germination rate. Also,
that A. morio subsp. morio had the best germination rate by
88.91%. Protocorms developed in the sixteenth week after sowing. Soil was
collected from the natural habitat of each species and was used as a potting
substrate, and this helped orchids to pass their initial acclimatization stage.
Regeneration success of orchids at in vitro conditions could be
increased by using SV growth medium, following their natural hardening off
cycle and using specific substrates from their respective habitats.

Keywords

Micropropagation, Salep orchids, Orchimax, Knudson C, Svante Malmgren

References

• Reference1 Assist. Prof. Dr. BEHİYE BANU BİLGEN Namık Kemal University, Faculty of Agriculture, Department of Agricultural Biotechnology, Campus/ Tekirdağ (0282) 250 - 2291, [e-mail], bbilgen.cv.nku.edu.tr
• Reference2 Prof. Dr. Aynur GÜREL Ege Univ. Dept of Bioengineering IZMIR [e-mail], [e-mail]
• Reference3 Prof. Dr. Ayşe Filiz GÜREL İstanbul University, Faculty of Science, Dept. of Molecular Biology and Genetics PK 34134 Vezneciler, Fatih/İstanbul - TURKEY Telephone: [phone], Fax: [phone] [e-mail]