Theraupeutic potency of benfotiamine against methotrexate-induced kidney injury and irisin immunoreactivity

Year 2020, Volume: 9 Issue: 2, 244 - 253, 30.12.2020

Alper Yalçın , Ali Gürel

Abstract

Amaç: Benfotiamine (BFT), antioksidatif etkilere sahip etkili bir ajandır. Bu çalışmada, BFT'nin metotreksat (MTX) kaynaklı böbrek hasarı ve irisinin immünoreaktivitesi üzerindeki etkileri araştırılmıştır. Yöntem: Toplam 28 sıçan dört eşit gruba ayrıldı: Kontrol (herhangi bir tedavi uygulanmadı), BFT (50 mg / kg BFT oral gavaj ile verildi), MTX (20 mg / kg MTX intraperitoneal yolla verildi) ve MTX + BFT. Çalışmanın sonunda, alınan böbrek dokuları rutin histolojik takip serilerinden geçirildi ve parafin bloklara gömüldü. Histopatolojik inceleme için hematoksilen & eozin, irisin ve kaspaz 3 için streptavidin-biotin-peroksidaz kompleks yöntemi parafin bloklardan alınan kesitlere uygulandı. Toplam antioksidan seviyesi (TAS) ve toplam oksidan seviyesi (TOS) Rel Assay kitleri ile belirlendi. Bulgular: MTX'in histopatolojik hasara, irisin ve kaspaz-3 immünoreaktivitesinde önemli bir artışa neden olduğu gözlendi. Biyokimyasal olarak, MTX verilen hayvanlarda toplam oksidan seviyesinde (TOS) önemli artış ve toplam antioksidan seviyesinde (TAS) düşüş belirlendi. BFT tedavisinin histopatolojik hasarı iyileştirdiği, TOS ve kaspaz-3 immünoreaktivitesini önemli ölçüde azalttığı, TAS düzeylerini artırdığı ve irisin immünoreaktivitesini önemsiz ölçüde azalttığı bulundu. Sonuç: Sonuç olarak BFT, MTX'in neden olduğu böbrek hasarını önlemede koruyucu etkiler sergilemiştir.

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