Development of LC-MS/MS Method of Measurement of Direct Ethanol Biomarkers Ethyl Glucuronid and Ethyl Sulfate in Urine and Serum Matriches in Detection and Tracking of Alcohol Use
Abstract
In this study, it was aimed to develop an easy and cost-effective analysis methodology that provides the qualitative and quantitative detection of
EtG and EtS in urine and serum matrices.
Materials and Methods: For the simultaneous quantification of EtG and EtS biomarkers in urine and serum, an LC-MS/MS method with fast and reliable sample preparation protocols with 5 minutes analysis time has been developed and validated. Two product ions along with the precursor ions of both alcohol metabolites were monitored and the deuteron-derivative of EtG was used as an internal standard, for increasing the reliability of the results.
Results: The validation parameters and obtained values were investigated based on international guidelines.They were found within acceptable ranges. Thus, sensitive determination of EtG and EtS in urine/serum samples was achieved with a simple sample preparation protocol which could be automated.
Conclusion: In this study, a simple LC-MS/MS based analytical methodology has been developed for the simultaneous quantitative analysis of EtG and EtS in urine and serum matrices. Time-consuming and costly processes such as chemical modification (derivatization), matrix cleaning with SPEcartridges or concentration were not applied at any step of sample preparation. The developed analytical method has been validated within theframework of internationally accepted biochemical analytical method development guidelines.
Keywords
Alcohol Biomarkers, Ethyl Glucuronide, Ethyl Sulphate, Alcohol Use Disorder, LC-ESI-MS/MS
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