MHC-CLASS II CELLS IN THE OVIDUCT PLAY A ROLE DURING THE MENSTRUAL CYCLE AND EARLY PREGNANCY?

Year 2001, Volume: 54 Issue: 3, 177 - 182, 30.09.2001

Vissun Sevinç İnan

https://izlik.org/JA96NX82RU

Abstract

The epitheliurn of the oviduct may play significant roles in defending oviductal tissue and fertilized ovum from infection. The aim of this study was to determine the role of MHC-class II cells by analyzing them via HLA-DR immunoreactivity in the human oviduct epithelium during the menstrual cycle and early pregnancy. Oviducts from 21 healthy women undergoing tubal sterilization were collected and fixed in 10% formalin. After dehydration in ethanol, the oviducts were embedded in paraffin.
Sections were immunostained with monoclonal mouse-anti human HLA-DR antibody, and immunoreactivity was determined using immunoperoxidase technique under light microscopy. While increased HLA-DR immunoreactivity was observed in columnar epithelial ceils during the proliferatory
phase of the menstrual cycle, immunoreactivity was withdrawn from the epithelium in the secretory phase and in early pregnancy. These results indicated that human oviduct epithelium has positive immunoreactivity for MHC-II+ cells and that increased immunoreactivity correlated with ovulation. Varied distribution of HLA-DR immunoreactivity in the oviduct epithelium may play a significant role in the regulation of local immunocompetence during the menstrual cycle. increased HLA-DR immunoreactivity in the proliferatory phase may be needed against invading microorganisms. At the same time, a decrease in HLA-DR
immunoreactivity in the secretory phase and early pregnancy may be required to endure preimplantation of the foreign

Keywords

Oviduct , HLA-DR , Immunohistochemistry

Project Number

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TUBA UTERINADAKI MI-tC-CIaas HÜCRELERiNlN, MENSTRUAL SİKLUS VE ERKEN GEBELİK DÖNEMLERİNDEKİ ROIÜ

https://izlik.org/JA96NX82RU

Abstract

Tuba uterina lmenini déseyen epitel dokusu, bu bölgeye gelebilecek patojenlere karşı ilk bariyeri oluşturmaktadır. Doku uygunluk kompleksi-MHC (Major Histocompatibility Kompleks}
class {1 hücrelerinin, bu bölgede varlığının gösterilmesi lokal imümun cevap sırasında, tuba uterinanın antijen sunucu olarak rol
oynayabileceğini değinmektedir.
Bu ¢alışmada, menstrual siklus ve erken gebelik döneminde
insan tuba uterina epitelindeki doku uygunluk kompleksi-li hicrelerinin rolindin, HLA-DR immunoreaktivitesi ile değerlendirilemesi amaçlanmıştır. Tuba uterina örnekleri, 21 sağlıklı kadından,
mini laparotomi ile Pomeroy usuld yapılan tip ligasyonunda alınarak % 10 formalinde tesbit edildi. Alkol serileri ile dehidrate edildikten sonra, ksilende seffaflandirilan dokular parafin bloklara gdmildi. Inimiinohistokimyasal boyama icin, 6 mikron kalınlığındaki kesitler deparafinize edildi. Anti HLA-DR birincil antiokoru uygulandıktan sonra, immunoperoksidaz tekniği kullanılarak imminoreaktivite isik mikroskobu altında değerlendirildi,
Tuba uterinanın tek sıra prizmatik epitel hücrelerinde, mensturual siklusun proliferasyon fazında HLA-DR immunoreaktivitesi
belirgin pozitif olarak gözlenirken, sekresyon fazında ve erken
gebelik döneminde ise immunoreaktivitenin azaldığı saptand).
Bu ¢alışma sonucunda, tuba uterinanın ovulasyon öncesi dönemde positif HLA-DR immunoreaktivitesinin mikroorganizmalara karst gerekli ümmün cevabın sağlanmasında; ovulasyon sonrası dönemde azalmış immunoreaktivitenin ise implantasyon öncesi yabancı olan embriyonun optimal yaşaması ve korunmasına
yönelik gerekli lokal immunokompetansin sağanmasında yönelik gerekli lokal immunokompetansin sağlanmasında önemli rol oynayabileceği düşünülmüştür.

Keywords

Tuba Uterina , HLA-DR , Menstrual Siklus

Supporting Institution

-

Project Number

-

Thanks

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