The changes in adrenaline cells of adrenal medulla due to cold stress. Light and electron microscopic studies

Abstract

The alterations in the adreneline cells of the guinea pigs adrenal medullae which were exposed to cold stress were observed in light and electron microscopy. In the second experiment the most conspicuous difference was on the secretory granules. There was a partial deple- tion of the secretory granules in the second experiment (to cold ex- posure for 15 hours/day) and total depletion on the third experiment (to cold exposure for 210 hour in days). After cold stress, the quinea pigs were exposed to the room temperature and 48 hours later there was a marked increase in the number of the secretory granules and it was observed that the secretion was achieved by exocytosis. The alterations on the other cytoplasmic organelles such as lysosomes, lipids and multivesicular bodies were also discussed. It was also de- termined that the lead acetate staining which is used in the light microscopy techniques had notably ensured to differ the chromaffin cells from the cortex cells.

Keywords

• cortex
• secretory
• guinea pigs
• medullae

Soguk Stresi Uygulanan Sürrenal Medullası Adrenalin Hücrelerinde Işık Ve Elektron Mikroskobu Düzeyinde Gözlenen Değişikliklerin İncelenmesi

Abstract

Soğuk stresi etkisiyle kobay medullası adrenalin hücrelerindeki değişiklikler ışık ve elektron mikroskobu düzeyinde incelendi. Soğuk stressi altındaki ikinci deney süresinde medulla hücrelerinde en çok göze çarpan değişiklik salgı granüllerinde bulundu. Salgı granülleri- nin ikinci deney süresinde kısmen, üçüncü deney süresinde tamamen salındığı gözlendi. Soğuk etkisinden sonra normal oda ısısına alındık- tan 48 saat sonra yeni granül oluşumunun varlığı ile granül miktarın- da çoğalma görüldü. Salgılama biçiminin ekzositoz yoluyla olduğu göz- lendi. Sitoplazmadaki diğer organeller, lizozom, lipid ve multivezikü- ler cisimlerdeki değişiklikler literatür ışığında tartışıldı. Işık mikroskobik incelemelerde uyguladığımız kurşun asetat boyasının kromaffin hücrelerinin, korteks hücrelerinden belirgin biçimde ayırdolunmasını sağladığı belirtildi.

Keywords

• Soğuk stres
• kobay medullas
• elektron mikroskobu
• ekzositoz

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