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Purification of bovine liver cytoplasmic aldehyde dehydrogenase (aldh.) enzyme, investigation of its kinetic properties and inhibition by disulfiram

Year 1987, Volume: 40 Issue: 1 , 49 - 60 , 31.03.1987

İlker Durak

https://izlik.org/JA33RL88UJ

Abstract

Aldehyde dehydrogenase (Aldehyde oxidoreductase E.C. 1.2.1.3) is an enzyme that catalyzes the cravings of many aldehyde structures together with acetaldehyde formed as a result of alcohol metabolism. This enzyme has an important function in alcohol metabolism and in the emergence of the alcoholic type. Therefore, this cytoplasmic aldehyde dehydrogenase enzyme was purified from bovine heart, its kinetic properties at changed pHs and the nature of its inhibition by disulfiram, which is widely used in the treatment of alcoholics, were investigated. As a result, it was determined that the enzyme showed a higher activity at high pHs and disulfiram was a severe inhibitory substance for the enzyme.

Keywords

PURIFICATION OF CATTLE LIVER CYTOPLASMIC ALDEHYDE DEHYDROGENASE (ALDH.) ENZYME INVESTIGATION OF INFILTRATION BY DISULFIRAM WITH ITS KINETIC PROPERTIES human

References

  • Banett, AF. : Inhibition of dehydrogenase activity of sheep liver cytoplasmic aidehyde dehydrogenase hY magnesium ions Biochemisiry 22 : 716-779, 19B3,
  • Bergmeyer, H.U. : Methodes of Enzymatic Analysis : Verlag Chemie GMBH, Weinheim 5. 637-638 ve 652-633, 195.
  • Bergmeyer, H.U. : Methoden der Enzymatisohen Analyse ; Verlag Chemie Weinheim 5s. 184-186, 1974.
  • Crow, E., Kitson, M., Gibbon, M., Batt, D. : Intracellular localisation and properties of ALDH fyom sheep liver Biochim Biophys Acta 350, 121-128, 1974.
  • Dickinson, F.M. : The use of pH-gradient ion exchange chromatography to S&- perate sheep liver cytoplasmic aldehyde dehydrogenase İrom mitochondrial enzyme contamination And observations on cytoplasmic enzyme and disulfiram Biochem J 199 : 573-579, i98İ.
  • Erikkson, C.J.P. : The distribution and metabolism of gceteldehyde in rals during ethanol oxidation Biochem Pharmacol 26 : 249-252, 1977.
  • Göödde, H.W, : Pharmacogenetiçs of alcohol sensitivity Phar Biocham Behav 18 (1) : 161-166, 1983.
  • Greenfield, N.J., Fietruszko, P. : Two aldehyde dehydrogenases İrom human liver Biochem Biophy Acta, 483 : 35-45, 197.
  • Harada, 5. : Blood ethanol and acetaldehyde levels in japanas€ alcoholics and controls Phar Biochem Behav 18 (1) : 139-140, 1983.
  • Hartman, L. : Technigues Modernes de Laboratorie et Explanation Fonctionnels Tomel Lexparition Sci, Français, Paris, s. 132-136, 1871.
  • Reet, P.S.O. : The rate of ethanol elimination in fed rat liver. Cytoplaşmic and mitochondrial regulating factors. Helsingen Ylopisto S.F. 37-57, 1979.
  • Vallari, R. Pietruzsko, P. : Kinetic mechanism of the human cytoplasmic aldehyde dehydrogenase Arc, Biochim. Biophys. 212 (1) : 9-19, 1981.
  • 13. Vallari, A. Pietruzsko, P. : Human aldehyde delhiydrogenase : Mechanism of inhibition by disulfiram, Science 216 : 637-639, 1982.
  • 14. Von Wartburg : Folymorphism of alcohol and aldehyde dehydrogenase and their signilicance for âcetaldehyde toxicity. Pharm Biochem Behav 18 (1) 123-125, 1983

Sığır karaciğer sitoplazmik aldehit dehidrogenaz (aldh.) Enziminin saflaştırılması, kinetik özellikleri ile disulfiram tarafından inhibisyonun araştırılması

Year 1987, Volume: 40 Issue: 1 , 49 - 60 , 31.03.1987

İlker Durak

https://izlik.org/JA33RL88UJ

Abstract

Aldehit dehidrogenaz (Aldehit oksidoredüktaz E.C. 1.2.1.3), alkol metabolizması sonucu oluşan asetaldehit ile birlikte birçok aldehit yapısının isteklerini katalize eden bir enzimdir. Bu enzimin alkol metabolizmasında ve alkolik tipin ortaya çıkmasında önemli bir işlevi vardır. Bu nedenle, bu sitoplazmik aldehit dehidrogenaz enzimi sığır kalbinden saflaştırıldı, değişen pH'lardaki kinetik özellikleri ve alkoliklerin tedavisinde yaygın olarak kullanılan disülfiram tarafından inhibisyonunun doğası araştırıldı. Sonuç olarak, enzimin yüksek pH'larda daha yüksek bir aktivite gösterdiği ve disülfiramın enzim için ciddi bir inhibitör madde olduğu belirlendi.

Keywords

SIĞIR KARACİĞER SİTOPLAZMİK ALDEHİT DEHİDROGENAZ (ALDH.) ENZİMİNİN SAFLAŞTIRILMASI KİNETİK ÖZELLİKLERİ İLE DİSULFİRAM TARAFINDAN İNHİBİSYONUN ARAŞTIRILMASI

References

  • Banett, AF. : Inhibition of dehydrogenase activity of sheep liver cytoplasmic aidehyde dehydrogenase hY magnesium ions Biochemisiry 22 : 716-779, 19B3,
  • Bergmeyer, H.U. : Methodes of Enzymatic Analysis : Verlag Chemie GMBH, Weinheim 5. 637-638 ve 652-633, 195.
  • Bergmeyer, H.U. : Methoden der Enzymatisohen Analyse ; Verlag Chemie Weinheim 5s. 184-186, 1974.
  • Crow, E., Kitson, M., Gibbon, M., Batt, D. : Intracellular localisation and properties of ALDH fyom sheep liver Biochim Biophys Acta 350, 121-128, 1974.
  • Dickinson, F.M. : The use of pH-gradient ion exchange chromatography to S&- perate sheep liver cytoplasmic aldehyde dehydrogenase İrom mitochondrial enzyme contamination And observations on cytoplasmic enzyme and disulfiram Biochem J 199 : 573-579, i98İ.
  • Erikkson, C.J.P. : The distribution and metabolism of gceteldehyde in rals during ethanol oxidation Biochem Pharmacol 26 : 249-252, 1977.
  • Göödde, H.W, : Pharmacogenetiçs of alcohol sensitivity Phar Biocham Behav 18 (1) : 161-166, 1983.
  • Greenfield, N.J., Fietruszko, P. : Two aldehyde dehydrogenases İrom human liver Biochem Biophy Acta, 483 : 35-45, 197.
  • Harada, 5. : Blood ethanol and acetaldehyde levels in japanas€ alcoholics and controls Phar Biochem Behav 18 (1) : 139-140, 1983.
  • Hartman, L. : Technigues Modernes de Laboratorie et Explanation Fonctionnels Tomel Lexparition Sci, Français, Paris, s. 132-136, 1871.
  • Reet, P.S.O. : The rate of ethanol elimination in fed rat liver. Cytoplaşmic and mitochondrial regulating factors. Helsingen Ylopisto S.F. 37-57, 1979.
  • Vallari, R. Pietruzsko, P. : Kinetic mechanism of the human cytoplasmic aldehyde dehydrogenase Arc, Biochim. Biophys. 212 (1) : 9-19, 1981.
  • 13. Vallari, A. Pietruzsko, P. : Human aldehyde delhiydrogenase : Mechanism of inhibition by disulfiram, Science 216 : 637-639, 1982.
  • 14. Von Wartburg : Folymorphism of alcohol and aldehyde dehydrogenase and their signilicance for âcetaldehyde toxicity. Pharm Biochem Behav 18 (1) 123-125, 1983
There are 14 citations in total.

Details

Primary Language English
Subjects Medical Biochemistry and Metabolomics (Other)
Journal Section Research Article
Authors

İlker Durak This is me

Publication Date March 31, 1987
IZ https://izlik.org/JA33RL88UJ
Published in Issue Year 1987 Volume: 40 Issue: 1

Cite

APA Durak, İ. (1987). Purification of bovine liver cytoplasmic aldehyde dehydrogenase (aldh.) enzyme, investigation of its kinetic properties and inhibition by disulfiram. Ankara Üniversitesi Tıp Fakültesi Mecmuası, 40(1), 49-60. https://izlik.org/JA33RL88UJ
AMA 1.Durak İ. Purification of bovine liver cytoplasmic aldehyde dehydrogenase (aldh.) enzyme, investigation of its kinetic properties and inhibition by disulfiram. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 1987;40(1):49-60. https://izlik.org/JA33RL88UJ
Chicago Durak, İlker. 1987. “Purification of Bovine Liver Cytoplasmic Aldehyde Dehydrogenase (aldh.) Enzyme, Investigation of Its Kinetic Properties and Inhibition by Disulfiram”. Ankara Üniversitesi Tıp Fakültesi Mecmuası 40 (1): 49-60. https://izlik.org/JA33RL88UJ.
EndNote Durak İ (March 1, 1987) Purification of bovine liver cytoplasmic aldehyde dehydrogenase (aldh.) enzyme, investigation of its kinetic properties and inhibition by disulfiram. Ankara Üniversitesi Tıp Fakültesi Mecmuası 40 1 49–60.
IEEE [1]İ. Durak, “Purification of bovine liver cytoplasmic aldehyde dehydrogenase (aldh.) enzyme, investigation of its kinetic properties and inhibition by disulfiram”, Ankara Üniversitesi Tıp Fakültesi Mecmuası, vol. 40, no. 1, pp. 49–60, Mar. 1987, [Online]. Available: https://izlik.org/JA33RL88UJ
ISNAD Durak, İlker. “Purification of Bovine Liver Cytoplasmic Aldehyde Dehydrogenase (aldh.) Enzyme, Investigation of Its Kinetic Properties and Inhibition by Disulfiram”. Ankara Üniversitesi Tıp Fakültesi Mecmuası 40/1 (March 1, 1987): 49-60. https://izlik.org/JA33RL88UJ.
JAMA 1.Durak İ. Purification of bovine liver cytoplasmic aldehyde dehydrogenase (aldh.) enzyme, investigation of its kinetic properties and inhibition by disulfiram. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 1987;40:49–60.
MLA Durak, İlker. “Purification of Bovine Liver Cytoplasmic Aldehyde Dehydrogenase (aldh.) Enzyme, Investigation of Its Kinetic Properties and Inhibition by Disulfiram”. Ankara Üniversitesi Tıp Fakültesi Mecmuası, vol. 40, no. 1, Mar. 1987, pp. 49-60, https://izlik.org/JA33RL88UJ.
Vancouver 1.İlker Durak. Purification of bovine liver cytoplasmic aldehyde dehydrogenase (aldh.) enzyme, investigation of its kinetic properties and inhibition by disulfiram. Ankara Üniversitesi Tıp Fakültesi Mecmuası [Internet]. 1987 Mar. 1;40(1):49-60. Available from: https://izlik.org/JA33RL88UJ