Ultrastructural And Cytochemıcal Study On Intramıtochondrıal Granules Of Tubulus Proxımatıs Cells In Renal Cortex

Year 1975, Volume: 28 Issue: 3-4 , 478 - 490 , 30.09.1975

Aliye Erkoçak

https://izlik.org/JA86FS28PZ

Abstract

Electron microscope shows dense granules against electrons in the matrix of mitochondria (tintramitochondrial granules), The structural and functional characteristics of intramitochondrial granules are still a matter of debate. It has been suggested that they are related to ion transport into the cell (11, 6) or that some ions settle and accumulate on them (2, 4), that their structure is mainly composed of neutral lipids (8), and that they are phospholipids (12). In this study, the structure of intramitochondrial granules of the renal cortex proximal tubule and their relationship with the accumulation or transport of some cations were examined at the electron microscopic level with a cytochemical approach. For this purpose, the identification of lipid content, 2 - Enzymatic hydrolysis of nucleic acids, 3 - Removal of some ketones, 4 - Introduction of some divalent cations

Keywords

Intramitochondrial granule , Phospholipid , Renal tubular cell

Böbrek Korteksindeki Tubulus Proxımatıs Hücrelerinin İntramitokondrial Granülleri Üzerinde Ultrastrüktürel Ve Sitokimyasal Çalışma

https://izlik.org/JA86FS28PZ

Abstract

Elektron mikroskopla mitokondriyaların matriksi içinde elektronlara karşı yoğun granülalar görülür tintramitokondriyal granülalar), İntramitokondriyal granülaların yapısal ve fonksiyonel nitelikleri henüz tartışma konusudur. Bunların hücre içine iyon iletimi ile ilişkili olduğu (11, 6) veya üzerlerine
bazı iyonların oturup biriktiği (2, 4), yapılarının esas itibariyle nötral lipidlerden meydana geldiği (8), fosfolipid olduğu (12)
ileri sürülmüştür.
Bu çalışmada böbrek korteksi proksimal tubulusunun intra- mitokondriyal granülalarının yapısı ve bazı katyonların birik- tirilmesi veya iletimi ile ilişkisi sitokimyasal bir yaklaşımla elek- iron mikroskopik düzeyde incelendi. Bu amaçla Lipid içeriğin tanınması, 2 - Nükleik asitlerin enzimatik hidrolizi, 3 - Bazı ketyonların uzaklaştırılması, 4 - İki değerli bazı katyonların
. sokulması deneyleri yapıldı.

Keywords

İntramitokondriyal granül , Fosfolipid , Böbrek tubulus hücresi

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