Abstract
Roe-Kahn'ın serumda kolorimetrik kalsiyum tayini metodunda değişiklik yapıldı. Serumdaki kalsiyum önce kalsiyum okzalat şeklinde çöktürüldü. Bu çökelti asitte çözülerek tekrar trikalsiyum fosfat halinde çöktürüldü. Son aşamada Fiske-Subbarow renk reaksiyonu ile tayin gerçekleştirildi. Geliştirilen bu teknikle sadece 0,5 ml serum yeterli olmakta; bu miktar orijinal metodda kullanılandan dört kat daha azdır.
References
- 1 - ROE, J. H., KAHN, B. S. : The colorimetric determination of blood calcium. J. Biol. Chem. 81: 1, 1929.
- 2 - LYNCH, M. J., RAPHAEL, S., MELLOR, D., SPARE, D., HILLS, P., INWOOD, H. : Medical laboratory technology. W.B. Saunders Com. 128, 1963.
- 3 - WATSON, D., ROGERS, J. A.: Serum calcium determination with 1,2-diaminocyclohexane-N-tetra-acetate. Clin. Chim. Acta 8: 168, 1963.
- 4 - SEIVERD, C. E. : Chemistry for medical technologists. C. V. Mosby Com. 296, 1958.
- 5 - OSER, B. L., editor : Hawk's physiological chemistry. McGraw-Hill 14. ed. 1133, 1965.
- 6 - ROTA, C., GÖKHUN, I. : Copper tungstate as protein precipitant in urea determination by nesslerization method. Acta Med. Tur. 6: 45, 1969.
Abstract
We modified the colorimetric calcium estimation method of Roe and Kahn. In our approach, calcium in serum is initially precipitated as calcium oxalate. This precipitate is then dissolved in acid and subsequently re-precipitated as tricalcium phosphate. The colorimetric reaction of Fiske and Subbarow is applied for final quantification. Our improved technique uses only 0.5 ml of serum, which is a quarter of the volume used in the original method.
Keywords
Serum calcium Colorimetric method Roe-Kahn technique Calcium oxalate Tricalcium phosphate Fiske-Subbarow reaction
References
- 1 - ROE, J. H., KAHN, B. S. : The colorimetric determination of blood calcium. J. Biol. Chem. 81: 1, 1929.
- 2 - LYNCH, M. J., RAPHAEL, S., MELLOR, D., SPARE, D., HILLS, P., INWOOD, H. : Medical laboratory technology. W.B. Saunders Com. 128, 1963.
- 3 - WATSON, D., ROGERS, J. A.: Serum calcium determination with 1,2-diaminocyclohexane-N-tetra-acetate. Clin. Chim. Acta 8: 168, 1963.
- 4 - SEIVERD, C. E. : Chemistry for medical technologists. C. V. Mosby Com. 296, 1958.
- 5 - OSER, B. L., editor : Hawk's physiological chemistry. McGraw-Hill 14. ed. 1133, 1965.
- 6 - ROTA, C., GÖKHUN, I. : Copper tungstate as protein precipitant in urea determination by nesslerization method. Acta Med. Tur. 6: 45, 1969.
Details
| Primary Language | English |
|---|---|
| Subjects | Medical Biochemistry - Inorganic Elements and Compounds |
| Journal Section | Research Article |
| Authors | |
| Publication Date | June 30, 1970 |
| Published in Issue | Year 1970 Volume: 23 Issue: 3 |