Penicillinase Activity of Pathogenic Staphylococci and Its Relation to Penicillin Resistance
Abstract
The study investigates penicillin G resistance and penicillinase activity in 106 pathogenic staphylococcal strains (92 S. aureus, 14 S. albus) isolated from various clinical infections. Quantitative and semi-quantitative methods were used to evaluate enzyme activity. Results showed that 69 strains were resistant to penicillin with enzyme activities ranging from 0.1 to 667.5 μM/ml. Even some sensitive strains exhibited penicillinase, suggesting a role for intrinsic resistance in addition to enzymatic inactivation. The research discusses mechanisms of resistance, including genetic factors like plasmid-mediated enzyme induction, and compares efficacy of different testing methods. The study emphasizes the significance of penicillinase in therapy and prophylactic antibiotic selection.
Keywords
staphylococcus aureus, penicillin resistance, penicillinase, antibiotic resistance, microbiology, enzyme activity
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