Açlıkta Kobay Karaciğer Protein Miktarı ile Laktat Dehidrogenaz Enziminin Aktivitesinde Meydana Gelen Değişikliklerin Araştırılması

Abstract

Kobay karaciğer homogenâtından kısmen saflaştırılmış to- tal LDH ve LDH izoenzimleri aktivitesi ile karaciğerin çözünentotal protein miktarı tayin edildi. Açlıkta total protein miktarında, hızlı ve büyük bir azalma görülmesine karşılık total LDH ve LDH izoenzim aktivitelerinde önemli bir değişiklik tesbit edilememiştir. Sonuç olarak i LDH enzim-proteininin yarılanma süresinin karaciğerdeki çözünen proteinlerin yarılanma, süresinden daha uzun olduğu, ayrıca M alt ünitesini daha fazla oranda ihtiva, eden LDH izoenzimlerinde açlıkta, aktivitenin daha çok azaldığı müşahede edilmiştir.

Keywords

• Açlık
• Karaciğer Proteinleri
• LDH ve İzoenzimleri

Investigation Of Changes In Guinea Pig Liver Protein Content And Lactate Dehydrogenase Enzyme Activity During Starvation

Abstract

The activity of partially purified total LDH and LDH isoenzymes from guinea pig liver homogenate was determined, as was the soluble total protein content of the liver. While a rapid and significant decrease in total protein was observed during fasting, no significant changes were detected in total LDH and LDH isoenzyme activities. Consequently, it was observed that the half-life of the LDH enzyme-protein was longer than that of the soluble proteins in the liver, and that LDH isoenzymes containing a higher proportion of the M subunit exhibited greater activity reductions during fasting.

Keywords

• Fasting
• Liver Proteins
• LDH and Isoenzymes

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