Abstract
Rat liver and kidney alkaline phosphatases were partially purified. The effects and kinetics of methyl and ethyl parathion on these two forms of alkaline phosphatase were investigated for the first time. Methyl parathion activates kidney alkaline phosphatase, but has no effect on the liver form as an activator or inhibitor. i Ethyl parathion is alkaline to the liver and kidney. It inhibits both phosphatase. Extending the incubation period increases the inhibition to a certain level, and then it remains at a fairly constant value. With increasing inhibitor concentration, inhibition follows a typical sigmoid curve.
References
- 1 — BERSIN, T. Bioketalysatoren, Akademische Verlagsgesellschafit, Frankfurt a. Main, 1968.
- 2 — BUDDECKE, E, Grundriss der Biochemie, Walter de Gruyter, 1974.
- 3 — GERTİG, H, et al, The Effect of Aldrin, Dieldrin, Lindane, DDT, DDD, and DDE on The activity of alkaline and acid phosphatase, Dissert. Pharm. Pharmacol,, XXII, 5, 1971.
- 4 — HASCHEN, R.J. Enzymdiagnostik, Gustav Fischer Verlag, Stuttgart, 1970.
- 5 — JAKL, A, J. TULACH, Therapeutisehe Wirksamkelt von Esterasereaktivatoren bei der Vergiftung mit Organophosphonaten und ihre Grenzen., Hradci Krâlove, 14,2, 1971.
- 6 — KERN,R, et al, Einfluss organischer Insecticide auf Enzyme, Biochemische Zeitsohrift, 336, 1962. li
- 7 — KIERMEJER, F. B. PICHA, verhalten der alkalisehen Phosphatase der Milch gegenüber organischen Phosphorsaeursestern, Z. Lebensm. Ünters. Forsch., 142, 2, 1970. »
- 8 — KING, J., Practical Clinical Enzymology, D. Van Nostrand Co. Lid. London, 1965.
- 9— REHFELD, N, REICHELD, D., Analytische und praeparative Methoden der klinischen Biochemie, Verlag Chemie, 1973. i
- 10 — SEYLER, H. / THIERFELDER, Handbuch der physiologisch - und pathologisch chemischen Analyse 6. Band Enzyme Teil B. Springer Verlag, Berlin, 1964.
Abstract
Sıçan karaciğer ve böbrek alkali fosfatazları kısmen saflaştırıldı. Metil ve etil parationun alkali fosfatazın bu iki formu üzerindeki tesirleri ve kinetiği ilk defa tarafımızdan araştırıldı. Metil paration böbrek alkali fosfatazını aktive etmekte, karaciğerformu üzerine ise aktivatör ve inhibitör olarak hiç tesir etmemektedir. i Etil paration karaciğer ve böbrek alkali. fosfatazının her ikisini de inhibe etmektedir.İnkübasyon süresinin uzatılması inhibisyonu belli bir degerekadar artırmakta daha sonra oldukça sabit bir değerde kalmaktadır. Yükselen inhibitör konsantrasyonunda inhibisyon tipik sigmoid bir eğri çizmektedir.
References
- 1 — BERSIN, T. Bioketalysatoren, Akademische Verlagsgesellschafit, Frankfurt a. Main, 1968.
- 2 — BUDDECKE, E, Grundriss der Biochemie, Walter de Gruyter, 1974.
- 3 — GERTİG, H, et al, The Effect of Aldrin, Dieldrin, Lindane, DDT, DDD, and DDE on The activity of alkaline and acid phosphatase, Dissert. Pharm. Pharmacol,, XXII, 5, 1971.
- 4 — HASCHEN, R.J. Enzymdiagnostik, Gustav Fischer Verlag, Stuttgart, 1970.
- 5 — JAKL, A, J. TULACH, Therapeutisehe Wirksamkelt von Esterasereaktivatoren bei der Vergiftung mit Organophosphonaten und ihre Grenzen., Hradci Krâlove, 14,2, 1971.
- 6 — KERN,R, et al, Einfluss organischer Insecticide auf Enzyme, Biochemische Zeitsohrift, 336, 1962. li
- 7 — KIERMEJER, F. B. PICHA, verhalten der alkalisehen Phosphatase der Milch gegenüber organischen Phosphorsaeursestern, Z. Lebensm. Ünters. Forsch., 142, 2, 1970. »
- 8 — KING, J., Practical Clinical Enzymology, D. Van Nostrand Co. Lid. London, 1965.
- 9— REHFELD, N, REICHELD, D., Analytische und praeparative Methoden der klinischen Biochemie, Verlag Chemie, 1973. i
- 10 — SEYLER, H. / THIERFELDER, Handbuch der physiologisch - und pathologisch chemischen Analyse 6. Band Enzyme Teil B. Springer Verlag, Berlin, 1964.
Details
| Primary Language | English |
|---|---|
| Subjects | Medical Biochemistry - Proteins, Peptides and Proteomics |
| Journal Section | Research Article |
| Authors | |
| Publication Date | March 31, 1977 |
| IZ | https://izlik.org/JA75ND52BP |
| Published in Issue | Year 1977 Volume: 30 Issue: 1 |