LDH Isozymes in Myocardial Infarction

Abstract

Recent findings on the distribution patern of LDH isozymes in different dissues and the molecular and biochemical differences between these isoenzymes are described. A simple, reliable and cheap method that can be used for the measurement of LDH, activity increase in the diagnosis of myocard infarcts is described and compared with other methods.

Keywords

• LDH izozimleri
• Miyokard infarktüsü
• enzim aktivitesi ölçümü

Miyokard İnfarktüsünde Bir LDH İzozimi Üzerinde Çalışmalar

Abstract

Son yıllarda LDH izozimlerinin çeşitli dokulardaki dağılış paternleri ve bu izozimler arasındaki moleküller ve biokimyasal değişiklikler kısaca tarif edildi. Myokard infarktüsünün teşhisinde artan LDH aktivitesinin ölçülmesinde kullanılabilecek, basit, güvenilir ve ucuz bir metod tarif edildi ve diğer metodlarla mukayesesi yapıldı.

Keywords

• LDH isozymes
• Myocardial infarction
• Enzyme activity measurement

References

1. 1 — VESSELL, E. S8. AND BEARN, A.G.: Localization of lactic dehydrogenaz activity in serum fractions. Proc. Soc. Exp. Biol. Med. 94; 96- 1957.
2. 2 — WİELAND, T. AND PFLBİDERER, G.: Nachweis der Heterogenital von Milehsaure-Dehydrogenasen verschiedenen Urspungs durch Trogrelektrophoress, Blochem. Z. 329: 112- 1957.
3. 3 - MARKERT, C.L. AND MOLLER, F.: Multiple forms of enzymes, Tissue, Ontogenetic and species-specific pattern. Proc. Nat. Acad. Sci. (USA) 45: 758, 1959.
4. 4 - WİENNE, R.J.: Studies on Agar-jel electrophoresis. Brussels, Arscia Urtgaven N.V., 1959.
5. 5 - ORNSTEİN, L.: Disc electrophoresis. I. Background and theory. Ann. N.Y. Acad. Sci., 121: 321- 1964.
6. 6 - PRESTON, J.A., BRİERE, R.O., AND BATSAKİS, J.G.: Rapid electrophoretic separation of lactate dehydrogenase isoenzymes on cellulose acetate. Amer. J. Clin. Path. 43: 256- 1965.
7. 7 - WİELAND, T. AND PFLEİDERER, G.: Chemical differences between multiple forms of lactic acid dehydrogenase. Ann. N.Y. Acad. Sci., 94: 691, 1961.
8. 8 - PFLEİDERER, G., AND WACHAMUTH, E.D.: Die Heterogenität der Laktatdehydrogenase in Entwicklungsgeschichte und Pathologie des Menschen. Klin. Wschz. 39: 352- 1961.

9. 9 - MARKERT, C.L., AND APPELLA, R.: Immunochemical properties of lactate dehydrogenase isoenzymes. Ann. N.Y. Acad. Sci., 103: 915- 1963.
10. 10 - MARKERT, C.L.: Lactate dehydrogenase isoenzymes; dissociation and recombination of subunits. Science 140: 1329- 1963.
11. 11 - STARKWEATHER, W.H. AND SCHOCH, H.K.: Some observations on the lactate dehydrogenase of human neoplastic tissue. Biochim. Biophys. Acta 62: 440, 1962.
12. 12 - GERHARD, W., CLAUSEN, J., CHRISTENSEN, E.AND RİSHEDE, J.: Changes of LDH isoenzymes, esterases, acid phosphatases, and proteins in malignant and benign human brain tumors. Acta Neurol. Scand. 39: 85-111, 1963.
13. 13 - GOLDMAN, R.D., KAPLAN, N.D., AND HALL, T.C.: Lactate dehydrogenase in human neoplastic tissues. Cancer Res. 24: 389- 1964.
14. 14 - ROSOLKİ, S.B., WİLKİNSON, J.H.: Serum and hydroxybutyric dehydrogenase in diagnosis. J. Amer. Med. Ass. 189: 61, 1964.