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The Effect of Tween-60 (Polyoxyethylene Sorbitan Monostearate) Inducing Hyperplasia on Mouse Skin

Year 1971, Volume: 24 Issue: 1, 1 - 14, 28.02.1971

Kadri Bacacı

https://izlik.org/JA43LM64PG

Abstract

Dipole tip promotor maddelerden olan Tween-60 ın tekrarlayıcı tatbiklerinin fare derisinde meydana getirdiği hiperplazi etkisi araştırıldı ve neticeler literatürdekilerle karşılaştırıldı.
Tween-60 ın sebep olduğu epidermal hücre değişiklikleri bilhassa diferansiye hücrelerde asırı bir çoğalmayı sağlıyordu. Bunu temin eden en az tatbikat sayısı 2-3 gün veya tatbikat olarak bulundu. 6 gün tatbikatla epidermiste (+ +++) şiddetinde bir hiperplazi teessüs etti. Bu hiperplazi diffüz, muntazam ve hücrelerde şişme ödem, hücreler arası mesafelerde genişleme, köprücükler teşekkülü ile karakterli idi. Tween-60 tatbikatının kesilmesi ile bu hiperplazinin 2--4 gün sonra tedricen gerilemeye başladığı ve 7-10 gün içinde kaybolarak epidermisin normal histolojik görünümünü aldığı tespit edildi. Neticede, başlangıçta günlük tatbikatlarla epidermiste 3--4 günde iyi bir hiperplazinin meydana getirilebileceği ve bunun 22 3 günlük fasılalarla yapılacak tek Tween-60 tatbikatları ile ayni derecede muhafaza edilebileceği kanısına varıldı. Bu neticenin deneysel deri karsinogenezi çalışmalarında Promotion olayını tetkik etmede metod yönünden önemli olabileceğj düşünüldü.

Keywords

Hiperplazi Karsinogenez Polyoxyethylene Sorbitan Monostearate

References

  • 1 — BERENBLUM, 1. Irritation and Carcinogenesis. General Reviews. Arch. Path. 38 : 233, 1944.
  • 2 — BERENBLUM, 1. : Cocarcinogenesis. Brit. Med. Bull. 4 : 343, 1947
  • 3 — BERENBLUM, 1. : A Re-evaluation of The Concept of Cocarcinogenesis. Progr. Exp. Tumor Res. 11 • . 21, 1969
  • 4- BOCK, F. G. : Species Differences in Penetration and Absorbtion of Chemical Carcinogens. Nat. Cancer Inst. Monog. 10 : 361, 1963
  • 5 — CORNMAN, 1., GARGUS, J. L. and Mc DONALDS, M. : The Role of Proliferation in The Second Stage of Carcinogenesis. Cancer Res. 20 : 377, 1960
  • 6 — DAMMERT, K. : Zur Histology Der Chemichen Hautcarcinogenese tm Lichte Der Zwei Phasen Hypothese üntersucht On Mausen. Acta Path. Mic. Scand. Suppl. 124, 1957
  • 7— DAViBHADHANA, B. : Numerical Proportions of Different Cells in Mouse Epidermis During Early Methylcholanthrene Carcinogenesis. Cancer Res. 12 : 165, 1952 CO In
  • 8 — FREi, J. V. and STEPHENS, P. : The Correlation of Promotion of Tumour Growth and of Induction of Hyperplasia in Epidermal Two-Stage Carcinogenesis. Brit. J. Cancer 22 : 83, 1968.
  • 9 — GLUCKSMANN, A. : The Histogenesis of Benzpyrene-lnduced Epidermal Tumors in The Mouse. Cancer Res. 5 : 385, 1965
  • 10 — HOLSTÏ, P. : Tumor Promoting Effects of Some Long Chain Fatty Acids in Experimental Skin Carcinogenesis in The Mouse. Acta Path. Mic. Scand. 46 : 51, 1959
  • 11 — KLÏNKEN -RASMUSSEN, L. : Quantitative Morphological Studies on Cyclic Changes in The Mouse Skin With Special Reference to Carcinogenesis. 2. Mitotic Activity in The Epidermis and Hair Follicles During The First Two Hair Cycles. Acta Path. Mic. Scand. 35 : 523, 1954
  • 12 — MERENMÏES, L. : Zum Mechanismus Der Haut Tumorbildung Bei Mäusen. Untersucht Mit Hilfe Einiger Nichtionisierbaren Oberflächenactivan Tumorauslösersubstanzell. Acta Path. Mic. Scand. Suppl. 130 vol. 46, 1959
  • 13 — MOTTRAM, J. C. : A Developing Factor in Experimental Blastogenesis. J. Path. Bact. 56 : 181, 1944
  • 14 — SALAMAN, M. H. : Coca.rcinogenesis. Brit. Med. Bull. 14 : 116, 1958
  • 15 — SALAMAN, M. H. : Component of Local Carcinogenesis. Acta Unio Int. Cont. Canc. 17 • . 12, 1961
  • 16 — SETALA, H. : Tumor Promoting and Co-Carcinogenic Effects of Some Non-ionic, Lipophilic-Hydrophi1ic (Surface Active) Agents. An Experimental Study on Skin Tumors in Mice. Acta Path. Mic. Scand. Suppl. 115, 1956
  • 17 — SETALA, K, SETALA, H. and HOLSTI, P. : New and Physicochemically Well-Defined Group of Tumor-Promoting (Cocarcinagqnic) Agents For Mouse Skin. Science 120 : 1075, 1954
  • 18 — SETALA, K., HOLSTI, P. and LUNDBOM, S. : Criters d'Eva1uation Des Dangers De Cancerisation Les Action Co-Cancerigenes. Acta Unio Int. Cont. Canc. 13 : 280, 1957
  • 19 — SETALA, 1<., DAMMERT, K., SETALA, H., MERENMiES, L. and HOLSTI, P. : Morphologische Hautveränderungen Bei Mäusen Hervorgerufen Durch Nichtionisierbare Ober Flächenactive Tumorauslösende ('Tumor Promoting» Substanzen. Zeitsch. Krcbsforsch. 61 548, 1957
  • 20 — SETALA, K., MERENMiES, Ia., STJERNVALL, L., AHO, Y. and KAJANNE, P. : Mechanism of Experimental Tumorigenesis. I. Epidermal Hyperplasia in Mouse Coused by Locally Applied Tumor Initiator and Dipole-type Tumor Promotor. J. Nat. Cancer Inst. 23 : 925, 1959
  • 21 — SETALA, K., MERENMiES, L., STJERNVALL, L. and AHO, Y. : Mechanism of Experimental Tumorigenesis. Il. Effect of Mole Ratio Distribution of Span 60 and Span 20 Derivatives on Hyperplasia in Mouse Epidermis. J. Nat. Cancer Inst. 23 : 953, 1959
  • 22 —- SETALA, AHO, Y. and MERENMIES, L. : on The Ability of Clycerol Fatty Acid Esters to Produce Persisting Epidermal Hyperplasia in Mouse Ekin. Naturwiss. 46 : 328, 1959
  • 23 — SETALA, K., MERENMÎES, L., STJERNVALL, L., KAJANNE, P. and AHO, Y. Synthesis of Some Polyol Fatty Acid Ester Type Surfactants With Particular Attention to Their Tumour Promoting Property. Acta Unio Int. Cont. Canc. 15 : 224, 1959
  • 24 — SETALA, K., STJERNVALL, L., MERENMiES, L., HOLTSI, P., KAJANNE, P. and AHO, Y. : Morphological Alterations in Mouse Skin Induced by Synthesised Surfactant Type Tumour Promoters. Acta Unio Int. Cont. Canc 15 : 680, 1959
  • 25 — SETALA, K. : Progress in Carcinogenesis, Tumor-enhancing Factors. A Bioassay of Skin Tumor Formation. Progr. Exp. Tumor Res. 225, 1960
  • 26 — SETALA, MERENMíES, L., STJERNVALL, L., NYHOLM, M. and AHO, Y. : Mechanism Of Experimental Tumorigenesis. V. Ultrastructural Alterations in Mouse Epidermis Caused by Span 60 and Tween 60 Type Agents. J. Nat. Cancer Inst. 24 : 355, 1960
  • 27 — SETALA, K. : Nature and Mechanism of Tumour Promotion in Skin Carcinogenesis in Mice. Acta Unio Int. Cont. Canc. 17 : 32, 1961
  • 28 — SETALA, R., NiSKANEN, E. E., NŸYSSÖNEÑ, O. and LiNDROOS, B. : Zum Mechanismus Der Arifiziellen Geschwulstgenese. XII. Licht und Polarizations Mikroskopische Umwandlungen Der Epidermis Tumor Empfindlicher CFI - und CFW -Mäuse Der Swiss - Gruppe, Hervargerufen Durch Tween 60. Acta Path. Mic. Scand. Suppl. 155 : 47, 1962
  • 29 SKJAEGGESTAD, Ø : Experimental Epidermal Hyperplasia in Mice Relation to Carcinogenesis. Acta Path. Mic. Scand. Suppl. 169, 1964
  • 30 WALPOLE, A. L. : Initiation and Promotion in Carcinogenesis. Mec hanism of Action. Ciba Foundation Symposium on Carcinogenesis, s : 41, Little, Brown 1958
  • 31 — WEiSSMANN, G., TROLL, W., VAN DUUREN, B. and SESSA, G. A New Action of Tumor-promoting Agents : Lysis of Cell and Intracellular Membranes. J. Clin. Invest. 46 : 1132, 1967

Tween-60 (Polyoxyethylene Sorbitan Monostearate) Fare Derisinde Hiperplazi Doğuran Etkisi

Year 1971, Volume: 24 Issue: 1, 1 - 14, 28.02.1971

Kadri Bacacı

https://izlik.org/JA43LM64PG

Abstract

Hyperplastic effect of repeated applications of Tween-60 onto the mouse skin has been investigated, and results compared with those reported in the literatüre.
The cellular changes in the epidermis were consisted of marked proliferation of the differentiated spinal cells. The least number of the application causing hiperplasia of epidermis was found to be 2-3 times. 6 applications were produced ( I I + ) severe epi„ dermal hiperplasia. This was diffuse, regular, and characterised by cellular swelling, edema, expansion in the intercellular spaces, and also exageration of the intercellular bridges. With the interruption of Tween-60 applications, this hyperplasia was regressed gradually and disappeared through seventh to tenth days, and the histologic appearence of the epidermis was returned to mal. In this way, it is said that, a nice epidermal hyperplasia can be produced by daily application of Tween-60 within 3-4 days. Its severity can be keep up at the same level for a long time by single application every other day or 3 days interval. This finding was found to be important in respect to method of determination of promotion in the studies on experimental carcinogenesis.

Keywords

Hyperplasia Carcinogenesis Polyoxyethylene Sorbitan Monostearate

References

  • 1 — BERENBLUM, 1. Irritation and Carcinogenesis. General Reviews. Arch. Path. 38 : 233, 1944.
  • 2 — BERENBLUM, 1. : Cocarcinogenesis. Brit. Med. Bull. 4 : 343, 1947
  • 3 — BERENBLUM, 1. : A Re-evaluation of The Concept of Cocarcinogenesis. Progr. Exp. Tumor Res. 11 • . 21, 1969
  • 4- BOCK, F. G. : Species Differences in Penetration and Absorbtion of Chemical Carcinogens. Nat. Cancer Inst. Monog. 10 : 361, 1963
  • 5 — CORNMAN, 1., GARGUS, J. L. and Mc DONALDS, M. : The Role of Proliferation in The Second Stage of Carcinogenesis. Cancer Res. 20 : 377, 1960
  • 6 — DAMMERT, K. : Zur Histology Der Chemichen Hautcarcinogenese tm Lichte Der Zwei Phasen Hypothese üntersucht On Mausen. Acta Path. Mic. Scand. Suppl. 124, 1957
  • 7— DAViBHADHANA, B. : Numerical Proportions of Different Cells in Mouse Epidermis During Early Methylcholanthrene Carcinogenesis. Cancer Res. 12 : 165, 1952 CO In
  • 8 — FREi, J. V. and STEPHENS, P. : The Correlation of Promotion of Tumour Growth and of Induction of Hyperplasia in Epidermal Two-Stage Carcinogenesis. Brit. J. Cancer 22 : 83, 1968.
  • 9 — GLUCKSMANN, A. : The Histogenesis of Benzpyrene-lnduced Epidermal Tumors in The Mouse. Cancer Res. 5 : 385, 1965
  • 10 — HOLSTÏ, P. : Tumor Promoting Effects of Some Long Chain Fatty Acids in Experimental Skin Carcinogenesis in The Mouse. Acta Path. Mic. Scand. 46 : 51, 1959
  • 11 — KLÏNKEN -RASMUSSEN, L. : Quantitative Morphological Studies on Cyclic Changes in The Mouse Skin With Special Reference to Carcinogenesis. 2. Mitotic Activity in The Epidermis and Hair Follicles During The First Two Hair Cycles. Acta Path. Mic. Scand. 35 : 523, 1954
  • 12 — MERENMÏES, L. : Zum Mechanismus Der Haut Tumorbildung Bei Mäusen. Untersucht Mit Hilfe Einiger Nichtionisierbaren Oberflächenactivan Tumorauslösersubstanzell. Acta Path. Mic. Scand. Suppl. 130 vol. 46, 1959
  • 13 — MOTTRAM, J. C. : A Developing Factor in Experimental Blastogenesis. J. Path. Bact. 56 : 181, 1944
  • 14 — SALAMAN, M. H. : Coca.rcinogenesis. Brit. Med. Bull. 14 : 116, 1958
  • 15 — SALAMAN, M. H. : Component of Local Carcinogenesis. Acta Unio Int. Cont. Canc. 17 • . 12, 1961
  • 16 — SETALA, H. : Tumor Promoting and Co-Carcinogenic Effects of Some Non-ionic, Lipophilic-Hydrophi1ic (Surface Active) Agents. An Experimental Study on Skin Tumors in Mice. Acta Path. Mic. Scand. Suppl. 115, 1956
  • 17 — SETALA, K, SETALA, H. and HOLSTI, P. : New and Physicochemically Well-Defined Group of Tumor-Promoting (Cocarcinagqnic) Agents For Mouse Skin. Science 120 : 1075, 1954
  • 18 — SETALA, K., HOLSTI, P. and LUNDBOM, S. : Criters d'Eva1uation Des Dangers De Cancerisation Les Action Co-Cancerigenes. Acta Unio Int. Cont. Canc. 13 : 280, 1957
  • 19 — SETALA, 1<., DAMMERT, K., SETALA, H., MERENMiES, L. and HOLSTI, P. : Morphologische Hautveränderungen Bei Mäusen Hervorgerufen Durch Nichtionisierbare Ober Flächenactive Tumorauslösende ('Tumor Promoting» Substanzen. Zeitsch. Krcbsforsch. 61 548, 1957
  • 20 — SETALA, K., MERENMiES, Ia., STJERNVALL, L., AHO, Y. and KAJANNE, P. : Mechanism of Experimental Tumorigenesis. I. Epidermal Hyperplasia in Mouse Coused by Locally Applied Tumor Initiator and Dipole-type Tumor Promotor. J. Nat. Cancer Inst. 23 : 925, 1959
  • 21 — SETALA, K., MERENMiES, L., STJERNVALL, L. and AHO, Y. : Mechanism of Experimental Tumorigenesis. Il. Effect of Mole Ratio Distribution of Span 60 and Span 20 Derivatives on Hyperplasia in Mouse Epidermis. J. Nat. Cancer Inst. 23 : 953, 1959
  • 22 —- SETALA, AHO, Y. and MERENMIES, L. : on The Ability of Clycerol Fatty Acid Esters to Produce Persisting Epidermal Hyperplasia in Mouse Ekin. Naturwiss. 46 : 328, 1959
  • 23 — SETALA, K., MERENMÎES, L., STJERNVALL, L., KAJANNE, P. and AHO, Y. Synthesis of Some Polyol Fatty Acid Ester Type Surfactants With Particular Attention to Their Tumour Promoting Property. Acta Unio Int. Cont. Canc. 15 : 224, 1959
  • 24 — SETALA, K., STJERNVALL, L., MERENMiES, L., HOLTSI, P., KAJANNE, P. and AHO, Y. : Morphological Alterations in Mouse Skin Induced by Synthesised Surfactant Type Tumour Promoters. Acta Unio Int. Cont. Canc 15 : 680, 1959
  • 25 — SETALA, K. : Progress in Carcinogenesis, Tumor-enhancing Factors. A Bioassay of Skin Tumor Formation. Progr. Exp. Tumor Res. 225, 1960
  • 26 — SETALA, MERENMíES, L., STJERNVALL, L., NYHOLM, M. and AHO, Y. : Mechanism Of Experimental Tumorigenesis. V. Ultrastructural Alterations in Mouse Epidermis Caused by Span 60 and Tween 60 Type Agents. J. Nat. Cancer Inst. 24 : 355, 1960
  • 27 — SETALA, K. : Nature and Mechanism of Tumour Promotion in Skin Carcinogenesis in Mice. Acta Unio Int. Cont. Canc. 17 : 32, 1961
  • 28 — SETALA, R., NiSKANEN, E. E., NŸYSSÖNEÑ, O. and LiNDROOS, B. : Zum Mechanismus Der Arifiziellen Geschwulstgenese. XII. Licht und Polarizations Mikroskopische Umwandlungen Der Epidermis Tumor Empfindlicher CFI - und CFW -Mäuse Der Swiss - Gruppe, Hervargerufen Durch Tween 60. Acta Path. Mic. Scand. Suppl. 155 : 47, 1962
  • 29 SKJAEGGESTAD, Ø : Experimental Epidermal Hyperplasia in Mice Relation to Carcinogenesis. Acta Path. Mic. Scand. Suppl. 169, 1964
  • 30 WALPOLE, A. L. : Initiation and Promotion in Carcinogenesis. Mec hanism of Action. Ciba Foundation Symposium on Carcinogenesis, s : 41, Little, Brown 1958
  • 31 — WEiSSMANN, G., TROLL, W., VAN DUUREN, B. and SESSA, G. A New Action of Tumor-promoting Agents : Lysis of Cell and Intracellular Membranes. J. Clin. Invest. 46 : 1132, 1967
There are 31 citations in total.

Details

Primary Language English
Subjects Anatomy
Journal Section Research Article
Authors

Kadri Bacacı This is me

Publication Date February 28, 1971
IZ https://izlik.org/JA43LM64PG
Published in Issue Year 1971 Volume: 24 Issue: 1

Cite

APA Bacacı, K. (1971). Tween-60 (Polyoxyethylene Sorbitan Monostearate) Fare Derisinde Hiperplazi Doğuran Etkisi. Ankara Üniversitesi Tıp Fakültesi Mecmuası, 24(1), 1-14. https://izlik.org/JA43LM64PG
AMA 1.Bacacı K. Tween-60 (Polyoxyethylene Sorbitan Monostearate) Fare Derisinde Hiperplazi Doğuran Etkisi. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 1971;24(1):1-14. https://izlik.org/JA43LM64PG
Chicago Bacacı, Kadri. 1971. “Tween-60 (Polyoxyethylene Sorbitan Monostearate) Fare Derisinde Hiperplazi Doğuran Etkisi”. Ankara Üniversitesi Tıp Fakültesi Mecmuası 24 (1): 1-14. https://izlik.org/JA43LM64PG.
EndNote Bacacı K (February 1, 1971) Tween-60 (Polyoxyethylene Sorbitan Monostearate) Fare Derisinde Hiperplazi Doğuran Etkisi. Ankara Üniversitesi Tıp Fakültesi Mecmuası 24 1 1–14.
IEEE [1]K. Bacacı, “Tween-60 (Polyoxyethylene Sorbitan Monostearate) Fare Derisinde Hiperplazi Doğuran Etkisi”, Ankara Üniversitesi Tıp Fakültesi Mecmuası, vol. 24, no. 1, pp. 1–14, Feb. 1971, [Online]. Available: https://izlik.org/JA43LM64PG
ISNAD Bacacı, Kadri. “Tween-60 (Polyoxyethylene Sorbitan Monostearate) Fare Derisinde Hiperplazi Doğuran Etkisi”. Ankara Üniversitesi Tıp Fakültesi Mecmuası 24/1 (February 1, 1971): 1-14. https://izlik.org/JA43LM64PG.
JAMA 1.Bacacı K. Tween-60 (Polyoxyethylene Sorbitan Monostearate) Fare Derisinde Hiperplazi Doğuran Etkisi. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 1971;24:1–14.
MLA Bacacı, Kadri. “Tween-60 (Polyoxyethylene Sorbitan Monostearate) Fare Derisinde Hiperplazi Doğuran Etkisi”. Ankara Üniversitesi Tıp Fakültesi Mecmuası, vol. 24, no. 1, Feb. 1971, pp. 1-14, https://izlik.org/JA43LM64PG.
Vancouver 1.Kadri Bacacı. Tween-60 (Polyoxyethylene Sorbitan Monostearate) Fare Derisinde Hiperplazi Doğuran Etkisi. Ankara Üniversitesi Tıp Fakültesi Mecmuası [Internet]. 1971 Feb. 1;24(1):1-14. Available from: https://izlik.org/JA43LM64PG