Aluminyum Sülfat’ın “Ross” Cinsi Term Besi Civcivi Karaciğerinde Yarattığı Toksik Ve Infl amatuar Hasar Oxidative And Infl amatory Damage Of Aluminium Suplhate On The Liver Of Term Ross Broiler Chick

Abstract

Keywords

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Aluminyum Sülfat’ın “Ross” Cinsi Term Besi Civcivi Karaciğerinde Yarattığı Toksik Ve Inflamatuar Hasar

Abstract

Amaç: Döllenmiş Ross cinsi besi tavuk yumurtalarına kuluçkanın 1.gününde toksik dozda verilen Aluminyum Sülfat’ın term civciv karaciğerinde yaratmış olduğu muhtemel histolojik ve biyokimyasal etkilerin gösterilmesi. Gereç ve Yöntem: Döllenmiş Ross cinsi besi yumurtaları üç gruba ayrılarak deney grubu hava boşluğuna kuluçkanın 1.gününde 1000 mcg/0.1 ml serum fizyolojik içinde Aluminyum sülfat verildi. Sham grubuna sadece 0.1 ml serum fizyolojik uygulanırken, Kontrol grubuna herhangi bir işlem yapılmadı. Terme ulaşan civcivlerden karaciğerler alınarak ışık mikroskobu ile histolojik değişikliklere ve biyokimyasal olarak oksidatif reaksiyon indikatörleri olan Malond ialdehit (MDA) ve Glutatyon (GSH) düzeylerine bakıldı. Bulgular: Işık mikroskobunda deney grubunda karaciğerde yaygın inflamasyona, hepatosit hasarına, santral ven duvarında kalınlaşma ve pasif konjesyona ve sinuzoid yapısında bozulmaya rastlandı. Biyokimyasal olarak ise deney grubunda oksidatif reaksiyonu gösteren MDA yüksekliği ve GSH tükenmesi verilen dozda term Ross besi civcivlerinde ilk kez gösterildi. Hem histolojik hem de biyokimyasal olarak Sham ve Kontrol gruplarında herhangi bir değişikliğe rastlanmadı. Sonuç: Döllenmiş Ross cinsi besi tavuk yumurtalarına kuluçkanın 1.gününde 1000 mcg/0.1ml serum fizyolojik içinde verilen Aluminyum Sülfat term civciv karaciğerinde yaygın inflamatuar ve oksidatif hasara yol açmıştır.

Keywords

• aluminyum toksisitesi, karaciğer, histoloji, biyokimya, Malondialdehid, Glutatyon

References

1. Becaria A, Campbell A, Bondy SC. Aluminum as a toxicant. Toxicol Ind Health 2002 Aug;18:309-20.
2. Schetinger MRC, Morsch VM, Bohrer D. Aluminum: Interaction with nuc- leotides and nucleotidases and anal- ytical aspects of 1st determination. Struct Bond 2002; 104: 99-137.
3. Williams RJP. What is wrong with alu- minium? The J.D. Birchall Memorial Lecture. J Inorg Biochem 1999; 76: 81-88.
4. Yokel RA. Brain uptake, retention and efflux of aluminum and manga- nese. Coord Chem Rev 2002; 228: 97-113.
5. Osinska E, Kanoniuk D, Kusiak A. Aluminium hemotoxicity mecha- nisms. Ann Univ Mariae Curie Sklo- dowska [Med] 2004; 59: 411-416.
6. Yokel RA, Meurer KA, Skinner TL, et al. The 3-hydroxypyridin-4-ones more effectively chelate aluminum in a rabbit model of aluminum in- toxication than does desferrioxami- ne. Drug Metab Dispos 1996; 24: 105-111
7. Schetinger MR, Bonan CD, Mors- ch VM, et al. Effects of aluminium sulphate on delta-aminolevulinate dehydratase from kidney, brain, and liver of adult mice. Braz J Med Biol Res. 1999; 32, 761-766.
8. Kaneko N, Yasui H, Takada J, et al.

9. Kutlubay R, Oğuz EO, Güven C, et al. Histological and ultrastructural evidence for protective effects on aluminium-Induced kidney damage by intraperitoneal administration of alpha tocopherol. Int J Toxicol 2007; 26:95-101.
10. Kutlubay R, Oğuz EO, Can B, et al. Vitamin E protection from testicu- lar damage caused by intraperito- neal aluminium. Int J Toxicol 2007; 26:297-306.
11. Yousef MI. Aluminium-induced chan- ges in hemato-biochemical parame- ters, lipid peroxidation and enzyme activities of male rabbits: protective role of ascorbic acid. Toxicology. 2004 Jun 1;199:47-57.
12. Mahieu S, Millen N, González M, et al. Alterations of the renal function and oxidative stress in renal tissue from rats chronically treated with aluminium during the initial phase of hepatic regeneration. J Inorg Bio- chem 2005 Sep;99:1858-64 Orally administrated aluminum-mal- tolate complex enhances oxidative stress in the organs of mice. J Inorg Biochem 2004 Dec;98:2022-31
13. Osińska E, Kanoniuk D, Kusiak A. Aluminum hemotoxicity mecha- nisms. Ann Univ Mariae Curie Sklo- dowska [Med] 2004;59:411-6.
14. Han J, Han J, Dunn MA. Effect of di- etary aluminum on tissue nonheme iron and ferritin levels in the chick. Toxicology 2000 Jan 3;142:97-109.
15. Contini Mdel C, Ferri A, Bernal CA, et al. Study of iron homeostasis fol- lowing partial hepatectomy in rats with chronic aluminum intoxication. Biol Trace Elem Res 2007 Jan;115:31- 45.
16. Zaman K, Zaman A, Batcabe J. He- matological effects of aluminum on living organisms. Comp Biochem Physiol C 1993 Oct;106:285-93.
17. Whihelm M, Jaeger DE, Schüll-Cab- litz H, et al. Hepatic clearance and retention of aluminium: studies in the isolated perfused rat liver. Toxi- col Lett Volume 89, 1996 Dec313,257- 263
18. Kutlubay R, Oğuz EO, Abban G, et al. Amelioration of aluminium-indu- ced liver damage by vitamin E. Saudi Med J 2007; Vol. 28: 197-200
19. Han J, Han J, Dunn MA. Effect of di- etary aluminum on tissue nonheme iron and ferritin levels in the chick. Toxicology 2000 Jan 3;142:97-109.
20. Gonzalez MA, Alvarez Mdel L, Pisani GB, et al. Involvement of oxidative stress in the impairment in biliary secretory function induced by in- traperitoneal administration of alu- minum to rats. Biol Trace Elem Res 2007 Jun;116:329-48.
21. Golub MS, Gershwin ME, Donald JM, et al. Maternal and developmen- tal toxicity of chronic aluminum ex- posure in mice. Fundam and Appl Toxicol 1987 April; 8: 346-357.
22. Ebina Y, Okada S, Hamazaki S, et al. Liver, kidney, and central nervous system toxicity of aluminum given intraperitoneally to rats: A multip- le-dose subchronic study using alu- minum nitrilotriacetate. Toxicol and Appl Pharmacol 1984 Sept 15; 75: 211-218.
23. Okhawa H, Ohishi N. Assay for lipid peroxides in animal tissues by thio- barbituric acid reaction. Anal Bioc- hem 1979; 95: 351-358.
24. Moron MS, Depierre JW, Mannervik B. Level of glutathione, glutathione reductase and glutathione-S-transfe- rase activity in rat lung and liver. Bio- chem Biophys Acta 1979; 82: 67-78.
25. Stacchiotti A, Rodella LF, Ricci F, et al. Stress proteins expression in rat kidney and liver chronically exposed to aluminium sulphate. Histol Histo- pathol 2006 Feb;21:131-40.
26. Walker PR, LeBlanc J, Sikorska M. Ef- fects of aluminum and other cations on the structure of brain and liver chromatin Biochem 1989; 28: 3911- 3915.
27. Snyder JW, Serroni A, Savory J, et al. The absence of extracellular calcium potentiates the killing of cultured hepatocytes by aluminum maltolate. Arch Biochem Biophys 1995 Jan 10; 316:434-42.
28. De Marchi U, Mancon M, Battaglia 34. Missel JR, Schetinger MR, Gioda CR, V, et al. Influence of reactive oxygen species production by monoamine oxidase activity on aluminum-in- duced mitochondrial permeability transition. Cell Mol Life Sci 2004 Oct;61:2664-71.
29. Murakami K, Yoshino M. Aluminum decreases the glutathione regenera- tion by the inhibition of NADP-isocit- rate dehydrogenase in mitochond- ria. J Cell Biochem. 2004 Dec 15; 93:1267-71.
30. Abubakar MG, Taylor A, Ferns GA. Aluminium administration is associ- ated with enhanced hepatic oxidant stress that may be offset by dietary vitamin E in the rat. Int J Exp Pathol. 2003 Feb; 84:49-54.
31. Katyal R, Desigan B, Sodhi CP, et al. Oral aluminum administration and oxidative injury. Biol Trace Elem Res 1997 May;57:125-30.
32. Fulton B, Jeffery EH. The temporal relationship between hepatic GSH loss, heme oxygenase induction, and cytochrome P450 loss following in- traperitoneal aluminum administra- tion to mice. Toxicol Appl Pharmacol 1994 Aug; 127:291-7.
33. Abreo K, Sella M, Alvarez-Hernandez X, et al. Antioxidants prevent alu- minum-induced toxicity in cultured hepatocytes. J Inorg Biochem. 2004 Jun; 98:1129-34. et al. Chelating effects of novel py- rimidines in a model of aluminium intoxication. J Inorg Biochem 2005; 99: 1853-1857.
34. Nicholas JCB, Dawes PT, Davies JD, Freemon AJ. Persisting aluminium- related bone disease after cadaveric renal transplantation. Nephrol Dial Transplant 1999; 14: 202-204
35. Swain C, Chainy GB. In vitro stimula- tion of chick brain lipid peroxidation by aluminium, and effects of tiron, EDTA and some antioxidants. Indian J Exp Biol 2000 Dec; 38: 1231-5.