Deneysel ileusda indometasin kullanımının intestinal permeabilite ve bakteriyel translokasyon üzerine etkileri The effects ofindomethacine on intestinal permeability and bacterial translocation in in

Abstract

Keywords

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Deneysel ileusda indometasin kullanımının intestinal permeabilite ve bakteriyel translokasyon üzerine etkileri

Abstract

Amaç: Çalışmamızda mekanik intestinal obstrüksiyonlarda antiinflamatuvar bir ajan olarak indometasinin, intestinal permeabilite, bakteriyel translokasyon ve histopatolojik değişiklikler üzerindeki etkilerinin ortaya konulması amaçlanmıştır. Gereç ve Yöntem: Çalışmada her biri 10 hayvandan oluşan 4 grup oluşturulmuştur. Grup I (n=10) kontrol grubunu oluştururken, grup II (n=10) de terminal ileum ipek materyal ile 24 saat süresince bağlanarak deneysel ileus oluşturuldu. Grup III deki (n=10) hayvanlara 5 mg/kg subkutan indometasin uygulaması yapıldı. Grup IV de (n=10) ise intestinal obstrüksiyonu takiben indometasin uygulaması gerçekleştirildi. Grup IV de ileus oluşturulması ve indometasin uygulamaları grup II ve grup III deki ile aynı biçimde gerçekleştirildi. 51Cr ile işaretli etilen diamin tetraasetik asite (51Cr EDTA) olan intestinal permeabilite değişiklikleri laparatomilerden 24 saat sonra ve 6 saat süresince toplanan idrar örneklerinde değerlendirildi. Bakteriyel translokasyon mezenterik lenf nodları ve kan örnekleri kültüre edilerek değerlendirildi. Deney hayvanlarından intestinal doku örnekleri de alınarak histopatolojik değişiklikler ve kuru-yaş ağırlık değişiklikleri değerlendirildi. Bulgular: Deneysel ileus oluşturulmasını takiben geçekleştirilen indometasin uygulamasının, ileusa bağlı olarak artmış olan intestinal permeabiliteyi anlamlı biçimde azaltırken (%3.5±0.10 - %2.0±0.20, p

Keywords

• İntestinal obstrüksiyon, intestinal permeabilite, bakteriyel translokasyon, indometasin

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