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Effects of glucosamine on LPS/IFN-γ induced RAW 264.7 macrophage apoptosis

Year 2012, Volume: 65 Issue: 1, 11 - 18, 01.01.2012

Arzu Zeynep Karabay Aslı Koç Tülin Özkan Asuman Sunguroğlu Fügen Aktan Zeliha Büyükbingöl

https://doi.org/10.1501/Tipfak_000000802

Abstract

Keywords

-

References

  • Kerr J F, Wyllie A H. and Currie A R. Apoptosis: phenomenon implications in tissue kinetics. Br J Cancer 1972; 26, 239-257. biological with wide-ranging
  • Golstein P, Ojcius DM and Young JD. Cell death mechanisms and the immune system Immunol Rev 1991; 121, 29/65.
  • Orrenius S, Nicotera P Zhivotovsky B. Cell death mechanisms and their implications in toxicology. Toxicol Sci 2011; 119:3-19.
  • Dimmeler S, Zeiher AM. Nitric oxide and apoptosis: another paradigm for the double-edged role of nitric oxide. Nitric Oxide 1997; 1:275-81.
  • Snyder SH, Bredt DS. Biological roles of nitric oxide. Sci Am 1992; 266:68-71, 74- 7.
  • Simmons ML, Murphy S. Induction of nitric oxide synthase in glial cells. J Neuro- chem 1992; 59:897-905.
  • Magalhaes CR, Socodato RE, Paes-de- Carvalho R. Nitric oxide regulates the proliferation of chick embryo retina cells by mechanism. Int J Dev Neurosci 2006; 24:53- 60.
  • Gordon, S. Alternative activation of macrophages. Nat Rev Immunol 2003; 3:23-35.
  • Dalton DK, Pitts-Meek S, Keshav S, Figar IS, Bradley A, Stewart TA. Multiple defects of immune cell function in mice with disrupted interferon-gamma genes. Science 1993; 259:1739-42.
  • Ehrt S, Schnappinger D, Bekiranov S, Drenkow J, Shi S, Gingeras T.R, Gaaster- land T, Schoolnik G, Nathan C. Repro- gramming of the macrophage transcrip- tome in response to interferon-gamma and Mycobacterium tuberculosis: signal- ing roles of nitric oxide synthase-2 and phagocyte oxidase. J Exp Med 2001; 194:1123-40.
  • Albina JE, Cui S, Mateo RB, Reichner JS. Nitric oxide-mediated apoptosis in mu- rine peritoneal macrophages. J Immunol 1993; 150:5080-5.
  • Hortelano S, Alvarez AM, Bosca L. Nitric oxide induces tyrosine nitration and re- lease of cytochrome c preceding an in- crease of mitochondrial transmembrane potential in macrophages. FASEB J 1999; 13:2311-7.
  • Brockhaus F, Brüne B. p53 accumulation in apoptotic macrophages is an energy demanding process that precedes cytoch- rome c release in response to nitric oxi- de.Oncogene 1999; 11; 18:6403-10.
  • Lei K, Nimnual A, Zong WX, Kennedy NJ, Flavell RA, Thompson CB, Bar-Sagi D, Davis RJ. The Bax subfamily of Bcl2- related proteins is essential for apoptotic signal transduction by c-Jun NH(2)- terminal kinase. Mol Cell Biol 2002; 22:4929-42.
  • Messmer UK, Reimer DM, Reed JC, Brüne B. Nitric oxide induced poly(ADP- ribose) polymerase cleavage in RAW 264.7 macrophage apoptosis is blocked by Bcl-2. FEBS Lett 1996; 15;384:162-6.
  • Moncada S. The L-arginine: nitric oxide pathway, cellular transduction and immu- nological roles. Adv Second Messenger Phos- phoprotein Res 1993; 28:97-9.
  • Nathan C. Nitric oxide as a secretory product of mammalian cells. FASEB J 1992; 6:3051-64.
  • Odegaard JI, Ricardo-Gonzales RR, Red Eagle A, Vats D, Morel CR, Goforth MH., Subramanian V, Mukundan L, Ferrante AW, Chawla A. Alternative M2 activation of Kupffer cells by PPARdelta ameliorates resistance. Cell Metab 2008; 7:496-507. insulin
  • Alexander WS, Hilton DJ. The role of suppressors of cytokine signaling (SOCS) proteins in regulation of the immune response. Annu Rev Immunol 2004; 22:503- 29.
  • Block JA, Oegema TR, Sandy JD, Plaas A. The effects of oral glucosamine on joint health: is a change in research ap- proach needed? Osteoarthritis Cartilage 2010; 18:5-11.
  • Miller KL, Clegg DO. Glucosamine and chondroitin sulfate. Rheum Dis Clin North Am 2011; 7:103-18.
  • Rafi MM, Yadav PN, Rossi AO. Glucosamine inhibits LPS-induced COX- 2 and iNOS expression in mouse macrophage cells (RAW 264.7) by inhibition of p38-MAP kinase and transcription factor NF-kappaB. Mol Nutr Food Res 2007; 2051:587-93.
  • Rajapakse N, Kim MM, Mendis E, Kim SK. Inhibition of inducible nitric oxide synthase lipopolysaccharide-stimulated RAW264.7 cells by carboxybutyrylated glucosamine takes place via down-regulation of mitogen-activated mediated nuclear factor-kappaB signaling. Immunology 2008;123:348-57. in protein kinase- in lipopolysaccharide
  • Mossman B T. In vitro approaches for determining mechanisms of toxicity and carcinogenecity by asbestos in the gastrointestinal and respiratory tracts. Environ Health Perspect 1983; 53 : 155 – 161.
  • Yang YM, Liu GT. Damaging effect of cigarette smoke extract on primary cultured human umbilical vein endothelial cells and its mechanism. Biomed Environ Sci .2004; 17:121-34.
  • Ward C, Dransfield I, Chilvers ER, Haslett C, Rossi AG. Pharmacological manipulation of granulocyte apoptosis: potential therapeutic targets. Trends Pharmacol Sci 1999;20:503-9.
  • Gilroy DW, Lawrence T, Perretti M, Rossi AG. Inflammatory resolution: new opportunities for drug discovery. Nat Rev Drug Discov 2004; 3:401-16.
  • Messmer UK, Brüne B. Nitric oxide (NO) in apoptotic versus necrotic RAW 264.7 macrophage cell death: the role of NO-donor exposure, NAD+ content, and p53 accumulation. Arch Biochem Biophys. 1996; 327:1-10.
  • Borutaite V, Brown GC. Nitric oxide induces apoptosis via hydrogen peroxide, but necrosis via energy and thiol depletion. Free Radic Biol Med 2003; 1; 35:1457-68.
  • De Las Heras B, Hortelano S, Girón N, Bermejo P, Rodríguez B, Boscá L. Kaurane diterpenes protect against apoptosis and inhibition of phagocytosis in activated macrophages. Br J Pharmacol 2007; 152:249-55.
  • Tsi CJ, Chao Y, Chen CW, Lin WW. Aurintricarboxylic acid protects against cell death caused by lipopolysaccharide in macrophages by decreasing inducible nitric-oxide IkappaB kinase, extracellular signal- regulated kinase, and p38 mitogen- activated protein kinase inhibition. Mol Pharmacol 2002; 62:90-101. via
  • Jean-Baptiste EJ. Cellular mechanisms in sepsis. Intensive Care Med 2007; 22:63-72.
  • Calmels S, Hainaut P, Ohshima H. Nitric oxide induces conformational and func- tional modifications of wild-type p53 tu- mor suppressor protein.Cancer Res. 1997; 57:3365-9.
  • Messmer UK, Reimer DM, Reed JC, Brüne B. Nitric oxide induced poly(ADP- ribose) polymerase cleavage in RAW 264.7 macrophage apoptosis is blocked by Bcl-2. FEBS Lett 1996; 15;384:162-6.
  • Hortelano, S., Alvarez, A.M, Bosca, L. Nitric oxide induces tyrosine nitration and release of cytochrome c preceding an increase of mitochondrial trans- membrane potential in macrophages. FASEB J 1999; 13:2311-7.
  • Haas, M.J., Wong, N.C., Mooradian, A.D. Effect of glucosamine on apoli- poprotein AI mRNA stabilization and expression in HepG2 cells. Metabolism 2004; 53:766-7

Glukozaminin LPS/IFN-γ ile İndüklenen RAW 264.7 Makrofaj Apoptozu Üzerine Etkileri

Year 2012, Volume: 65 Issue: 1, 11 - 18, 01.01.2012

Arzu Zeynep Karabay Aslı Koç Tülin Özkan Asuman Sunguroğlu Fügen Aktan Zeliha Büyükbingöl

https://doi.org/10.1501/Tipfak_000000802

Abstract

Amaç: Apoptoz, doku homeostazisinin düzenlenmesi gibi normal fizyolojide ve çeșitli hastalıkların
patofizyolojisinde önemli rol oynayan genetik olarak programlanmıș bir hücre ölüm mekanizmasıdır.
Çeșitli hücre tiplerinde mikrobiyal ve viral patojenlere karșı bir savunma mekanizması
olarak ortaya çıkan iNOS enzim aktivasyonu ve nitrik oksit sentezi, ateroskleroz,
romatoid artrit, diyabet, septik șok, multipl sklerozis gibi inflamatuvar ve immün patolojilerde
önemli rol oynamakta, kontrolsüz yüksek düzeydeki nitrik oksit üretimi hücre ölümüne yol
açmaktadır. Yakın zamanda, osteoartrit gibi inflamatuvar bozukluklar için yararlı olduğu iddia
edilen glukozamin günümüzde klinik olarak kullanılmaktadır. Glukozaminin, bașlıca immün
sistem hücreleri olan makrofajların apoptozu üzerine olan etki mekanizması hakkında sınırlı
bilgi bulunmaktadır. Bu çalıșmada, farklı konsantrasyonlardaki glukozaminin, LPS/IFNγ ile aktive
edilmiș RAW 264.7 makrofajlar üzerindeki etkileri belirlenmiștir.
Materyal ve metod: RAW 264.7 makrofaj hücre serisi, LPS/IFNγ ile uyarım öncesinde
glukozamin varlığında ve yokluğunda kültür edildi. Nitrit düzeyleri, hücre canlılığı, kaspaz-3
aktivitesi, mitokondri membran potansiyeli tayini ve Annexin V-PI hücre boyaması ile akım
sitometrik analizler gerçekleștirilmiștir.
Sonuçlar ve tartıșma: Glukozaminin, nitrit düzeylerini inhibe ettiği, mitokondri membran
potansiyelini arttırdığı, kaspaz-3 enzim aktivitesini azalttığı ve anlamlı olarak antiapoptotik
etkiler sergilediği (p

Keywords

Apoptoz glukozamin nitrik oksit makrofaj

References

  • Kerr J F, Wyllie A H. and Currie A R. Apoptosis: phenomenon implications in tissue kinetics. Br J Cancer 1972; 26, 239-257. biological with wide-ranging
  • Golstein P, Ojcius DM and Young JD. Cell death mechanisms and the immune system Immunol Rev 1991; 121, 29/65.
  • Orrenius S, Nicotera P Zhivotovsky B. Cell death mechanisms and their implications in toxicology. Toxicol Sci 2011; 119:3-19.
  • Dimmeler S, Zeiher AM. Nitric oxide and apoptosis: another paradigm for the double-edged role of nitric oxide. Nitric Oxide 1997; 1:275-81.
  • Snyder SH, Bredt DS. Biological roles of nitric oxide. Sci Am 1992; 266:68-71, 74- 7.
  • Simmons ML, Murphy S. Induction of nitric oxide synthase in glial cells. J Neuro- chem 1992; 59:897-905.
  • Magalhaes CR, Socodato RE, Paes-de- Carvalho R. Nitric oxide regulates the proliferation of chick embryo retina cells by mechanism. Int J Dev Neurosci 2006; 24:53- 60.
  • Gordon, S. Alternative activation of macrophages. Nat Rev Immunol 2003; 3:23-35.
  • Dalton DK, Pitts-Meek S, Keshav S, Figar IS, Bradley A, Stewart TA. Multiple defects of immune cell function in mice with disrupted interferon-gamma genes. Science 1993; 259:1739-42.
  • Ehrt S, Schnappinger D, Bekiranov S, Drenkow J, Shi S, Gingeras T.R, Gaaster- land T, Schoolnik G, Nathan C. Repro- gramming of the macrophage transcrip- tome in response to interferon-gamma and Mycobacterium tuberculosis: signal- ing roles of nitric oxide synthase-2 and phagocyte oxidase. J Exp Med 2001; 194:1123-40.
  • Albina JE, Cui S, Mateo RB, Reichner JS. Nitric oxide-mediated apoptosis in mu- rine peritoneal macrophages. J Immunol 1993; 150:5080-5.
  • Hortelano S, Alvarez AM, Bosca L. Nitric oxide induces tyrosine nitration and re- lease of cytochrome c preceding an in- crease of mitochondrial transmembrane potential in macrophages. FASEB J 1999; 13:2311-7.
  • Brockhaus F, Brüne B. p53 accumulation in apoptotic macrophages is an energy demanding process that precedes cytoch- rome c release in response to nitric oxi- de.Oncogene 1999; 11; 18:6403-10.
  • Lei K, Nimnual A, Zong WX, Kennedy NJ, Flavell RA, Thompson CB, Bar-Sagi D, Davis RJ. The Bax subfamily of Bcl2- related proteins is essential for apoptotic signal transduction by c-Jun NH(2)- terminal kinase. Mol Cell Biol 2002; 22:4929-42.
  • Messmer UK, Reimer DM, Reed JC, Brüne B. Nitric oxide induced poly(ADP- ribose) polymerase cleavage in RAW 264.7 macrophage apoptosis is blocked by Bcl-2. FEBS Lett 1996; 15;384:162-6.
  • Moncada S. The L-arginine: nitric oxide pathway, cellular transduction and immu- nological roles. Adv Second Messenger Phos- phoprotein Res 1993; 28:97-9.
  • Nathan C. Nitric oxide as a secretory product of mammalian cells. FASEB J 1992; 6:3051-64.
  • Odegaard JI, Ricardo-Gonzales RR, Red Eagle A, Vats D, Morel CR, Goforth MH., Subramanian V, Mukundan L, Ferrante AW, Chawla A. Alternative M2 activation of Kupffer cells by PPARdelta ameliorates resistance. Cell Metab 2008; 7:496-507. insulin
  • Alexander WS, Hilton DJ. The role of suppressors of cytokine signaling (SOCS) proteins in regulation of the immune response. Annu Rev Immunol 2004; 22:503- 29.
  • Block JA, Oegema TR, Sandy JD, Plaas A. The effects of oral glucosamine on joint health: is a change in research ap- proach needed? Osteoarthritis Cartilage 2010; 18:5-11.
  • Miller KL, Clegg DO. Glucosamine and chondroitin sulfate. Rheum Dis Clin North Am 2011; 7:103-18.
  • Rafi MM, Yadav PN, Rossi AO. Glucosamine inhibits LPS-induced COX- 2 and iNOS expression in mouse macrophage cells (RAW 264.7) by inhibition of p38-MAP kinase and transcription factor NF-kappaB. Mol Nutr Food Res 2007; 2051:587-93.
  • Rajapakse N, Kim MM, Mendis E, Kim SK. Inhibition of inducible nitric oxide synthase lipopolysaccharide-stimulated RAW264.7 cells by carboxybutyrylated glucosamine takes place via down-regulation of mitogen-activated mediated nuclear factor-kappaB signaling. Immunology 2008;123:348-57. in protein kinase- in lipopolysaccharide
  • Mossman B T. In vitro approaches for determining mechanisms of toxicity and carcinogenecity by asbestos in the gastrointestinal and respiratory tracts. Environ Health Perspect 1983; 53 : 155 – 161.
  • Yang YM, Liu GT. Damaging effect of cigarette smoke extract on primary cultured human umbilical vein endothelial cells and its mechanism. Biomed Environ Sci .2004; 17:121-34.
  • Ward C, Dransfield I, Chilvers ER, Haslett C, Rossi AG. Pharmacological manipulation of granulocyte apoptosis: potential therapeutic targets. Trends Pharmacol Sci 1999;20:503-9.
  • Gilroy DW, Lawrence T, Perretti M, Rossi AG. Inflammatory resolution: new opportunities for drug discovery. Nat Rev Drug Discov 2004; 3:401-16.
  • Messmer UK, Brüne B. Nitric oxide (NO) in apoptotic versus necrotic RAW 264.7 macrophage cell death: the role of NO-donor exposure, NAD+ content, and p53 accumulation. Arch Biochem Biophys. 1996; 327:1-10.
  • Borutaite V, Brown GC. Nitric oxide induces apoptosis via hydrogen peroxide, but necrosis via energy and thiol depletion. Free Radic Biol Med 2003; 1; 35:1457-68.
  • De Las Heras B, Hortelano S, Girón N, Bermejo P, Rodríguez B, Boscá L. Kaurane diterpenes protect against apoptosis and inhibition of phagocytosis in activated macrophages. Br J Pharmacol 2007; 152:249-55.
  • Tsi CJ, Chao Y, Chen CW, Lin WW. Aurintricarboxylic acid protects against cell death caused by lipopolysaccharide in macrophages by decreasing inducible nitric-oxide IkappaB kinase, extracellular signal- regulated kinase, and p38 mitogen- activated protein kinase inhibition. Mol Pharmacol 2002; 62:90-101. via
  • Jean-Baptiste EJ. Cellular mechanisms in sepsis. Intensive Care Med 2007; 22:63-72.
  • Calmels S, Hainaut P, Ohshima H. Nitric oxide induces conformational and func- tional modifications of wild-type p53 tu- mor suppressor protein.Cancer Res. 1997; 57:3365-9.
  • Messmer UK, Reimer DM, Reed JC, Brüne B. Nitric oxide induced poly(ADP- ribose) polymerase cleavage in RAW 264.7 macrophage apoptosis is blocked by Bcl-2. FEBS Lett 1996; 15;384:162-6.
  • Hortelano, S., Alvarez, A.M, Bosca, L. Nitric oxide induces tyrosine nitration and release of cytochrome c preceding an increase of mitochondrial trans- membrane potential in macrophages. FASEB J 1999; 13:2311-7.
  • Haas, M.J., Wong, N.C., Mooradian, A.D. Effect of glucosamine on apoli- poprotein AI mRNA stabilization and expression in HepG2 cells. Metabolism 2004; 53:766-7
There are 36 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Arzu Zeynep Karabay This is me

Aslı Koç This is me

Tülin Özkan This is me

Asuman Sunguroğlu This is me

Fügen Aktan This is me

Zeliha Büyükbingöl This is me

Publication Date January 1, 2012
Published in Issue Year 2012 Volume: 65 Issue: 1

Cite

APA Karabay, A. Z., Koç, A., Özkan, T., Sunguroğlu, A., et al. (2012). Glukozaminin LPS/IFN-γ ile İndüklenen RAW 264.7 Makrofaj Apoptozu Üzerine Etkileri. Ankara Üniversitesi Tıp Fakültesi Mecmuası, 65(1), 11-18. https://doi.org/10.1501/Tipfak_000000802
AMA Karabay AZ, Koç A, Özkan T, Sunguroğlu A, Aktan F, Büyükbingöl Z. Glukozaminin LPS/IFN-γ ile İndüklenen RAW 264.7 Makrofaj Apoptozu Üzerine Etkileri. Ankara Üniversitesi Tıp Fakültesi Mecmuası. January 2012;65(1):11-18. doi:10.1501/Tipfak_000000802
Chicago Karabay, Arzu Zeynep, Aslı Koç, Tülin Özkan, Asuman Sunguroğlu, Fügen Aktan, and Zeliha Büyükbingöl. “Glukozaminin LPS/IFN-γ Ile İndüklenen RAW 264.7 Makrofaj Apoptozu Üzerine Etkileri”. Ankara Üniversitesi Tıp Fakültesi Mecmuası 65, no. 1 (January 2012): 11-18. https://doi.org/10.1501/Tipfak_000000802.
EndNote Karabay AZ, Koç A, Özkan T, Sunguroğlu A, Aktan F, Büyükbingöl Z (January 1, 2012) Glukozaminin LPS/IFN-γ ile İndüklenen RAW 264.7 Makrofaj Apoptozu Üzerine Etkileri. Ankara Üniversitesi Tıp Fakültesi Mecmuası 65 1 11–18.
IEEE A. Z. Karabay, A. Koç, T. Özkan, A. Sunguroğlu, F. Aktan, and Z. Büyükbingöl, “Glukozaminin LPS/IFN-γ ile İndüklenen RAW 264.7 Makrofaj Apoptozu Üzerine Etkileri”, Ankara Üniversitesi Tıp Fakültesi Mecmuası, vol. 65, no. 1, pp. 11–18, 2012, doi: 10.1501/Tipfak_000000802.
ISNAD Karabay, Arzu Zeynep et al. “Glukozaminin LPS/IFN-γ Ile İndüklenen RAW 264.7 Makrofaj Apoptozu Üzerine Etkileri”. Ankara Üniversitesi Tıp Fakültesi Mecmuası 65/1 (January 2012), 11-18. https://doi.org/10.1501/Tipfak_000000802.
JAMA Karabay AZ, Koç A, Özkan T, Sunguroğlu A, Aktan F, Büyükbingöl Z. Glukozaminin LPS/IFN-γ ile İndüklenen RAW 264.7 Makrofaj Apoptozu Üzerine Etkileri. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 2012;65:11–18.
MLA Karabay, Arzu Zeynep et al. “Glukozaminin LPS/IFN-γ Ile İndüklenen RAW 264.7 Makrofaj Apoptozu Üzerine Etkileri”. Ankara Üniversitesi Tıp Fakültesi Mecmuası, vol. 65, no. 1, 2012, pp. 11-18, doi:10.1501/Tipfak_000000802.
Vancouver Karabay AZ, Koç A, Özkan T, Sunguroğlu A, Aktan F, Büyükbingöl Z. Glukozaminin LPS/IFN-γ ile İndüklenen RAW 264.7 Makrofaj Apoptozu Üzerine Etkileri. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 2012;65(1):11-8.