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Effect of cigarette smoking on DNA damage according to nine comet assay parameters in female and male groups

Year 2012, Volume: 65 Issue: 1, 39 - 46, 01.01.2012
https://doi.org/10.1501/Tipfak_0000000805

Abstract

References

  • Church DF, Pryor WA. Free radical chemistry of cigarette smoke and it is toxicological Health Perspect 1985;64:111–126. Environ
  • Li W, Zhou J, Chen L, et al. Lysyloxidase, a critical intra- and extra-cellular target in the pathogenesis. Int J Environ Res Public Health 2011;8:161-184 smoke
  • International Early Lung Tumor Action Investigators
  • susceptibility Women's
  • carcinogens and survival after diagnosis of lung 96:180-184. (IELCAPI). to tobacco tumor. JAMA 2006;
  • American Tumor Society (ACS). Cancer facts and figures. American Tumor Society Atlanta. http://www.cancer.org/ Research/CancerFactsFigures/index 2010
  • Peto R, Lopez AD, Boreham J, et al. Mortality from tobacco in developed countries: indirect estimation from national 1992;339:1268-1278. Lancet
  • Halliwell B, Gutteridge JM. Free radicals and antioxidant protection: mechanisms and significance in toxicology and disease. Hum Toxicol 1988;7:7-13.
  • Mozaffarieh M, Konieczka K, Hauenstein D, et al. Half a pack of cigarettes a day more than doubles DNA breaks in circulating leukocytes. Tobacco Induced Diseases 2010;8:14.
  • Evans MD, Dizdaroglu M, Cooke MS. 2004. Oxidative DNA damage and disease: induction, repair and significance. Mutat Res 567:1–61.
  • Xiong P, Hu Z, Li C, et al. In vitro benzo[a]pyrenediol DNA aberrations in primary lymphocytes, smoking, and risk of squamous cell carcinoma of the head and neck. Int J Cancer 2007;121:2735-2740.
  • Hunt JD, Van der Hel OL, McMillan GP, et al. Renal cell carcinoma in relation to cigarette smoking: meta-analysis of 24 studies. Int J Cancer 2005;114:101-108.
  • Poli P, Buschini A, Spaggiari A, et al. DNA damage by tobacco smoke and some antiblastic drugs evaluated using the comet assay. Toxicol Lett 1999;108:267- 276.
  • Collins A, Cadet J, Epe B, et al. Problems in the measurement of 8-oxoguanine in human DNA. Report of a workshop, DNA oxidation, held in Aberdeen Carcinogenesis 1997;18:1833-1836.
  • Tice RR, Agurell E, Anderson D, et al. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 2000;35: 206–221.
  • Hoffmann H, Hogel J, Speit G. The effect of smoking on DNA effects in the comet assay: a meta-analysis. Mutagenesis 2005;20:455-466.
  • Faust F, KassieF, Knasmuller S, et al. The use of the alkaline comet assay with lymphocytes in human biomonitoring studies. Mutat Res 2004;566:209–229.
  • Singh NP, McCoy MT, Tice RR, et al. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988;175:184–191.
  • Hartmann A, Agurell E, Beevers C, et al. Recommendations for conducting the in vivo alkaline Comet assay. Mutagenesis 2003;18:45–51.
  • El-Zein RA, Monroy CM, Cortes A, et al. Rapid method for determination of DNA repairs capacity in human peripheral blood lymphocytes amongst smokers. BMC Cancer 2010;10:439.
  • Speit G, Witton-Davies T, Heepchantree W, et al. Investigations on the effect of cigarette smoking in the comet assay. Mu- tat Res 2003;542:33-42.
  • Hecht SS. Tobacco smoke carcinogens and lung cancer. J Natl Cancer Inst 1999;91:1194–1210.
  • Stabbert R, Voncken P, Rustemeier K, et al. Toxicological evaluation of an electrically heated cigarette. Part 2: chemical composition of mainstream smoke. J Appl Toxicol 2003;23:329–339.
  • Ferger B, Spratt C, Earl CD, et al. Effects of nicotine on hydroxyl free radical formation in vitro and on MPTP-induced neurotoxicity in vivo. Naunyn- Arch Pharmacol
  • Wetscher GL, Bagchi M, Bagchi D, et al. Free radical production in nicotine treated pancreatic tissue. Free Radic Biol Med 1995;18:877–882.
  • Pandey AK, Bajpayee M, Parmar D, et al. DNA damage in lymphocytes of rural Indian women exposed to biomass fuel smoke as assessed by the Comet assay. Environ Mol Mutagen 2005;45:435-441.
  • Collins AR, Ma AG, Duthie SJ. The kinetics of repair of oxidative DNA damage (strand breaks and oxidized pyrimidines) in human cells. Mutat Res 1995;336:69–77.
  • Hininger I, Chollat-Namy A, Sauvaigo S, et al. Assessment of DNA damage by comet assay on frozen total blood: method and evaluation in smokers and non-smokers. Mutat Res 2004;558: 75-80.
  • DeMarini DM. Genotoxicity of tobacco smoke and tobacco smoke condensate: a review. Mutat Res 2004;567: 447–474
  • Giovannelli L, Saieva C, Masala G, et al. Nutritional and lifestyle determinants of DNA oxidative damage: a study in a Mediterranean genesis 2002;23:1483-1489. Carcino
  • Singh NP, Danner DB, Tice RR, et al. DNA damage and repair with age in individual human lymphocytes. Mutat Res 1990;237:123-130.
  • Singh NP, Danner DB, Tice RR, et al. Basal DNA damage in individual human lymphocytes with age. Mutat Res 1991;256:1–6.
  • Liu X, Yao J, Pisha E, et al. Oxidative DNA damage induced by equine estrogen metabolites: role of estrogen receptor alpha. Chem Res Toxicol 2002;15:512- 519.
  • Ozcagli E, Sardas S, Biri A. Assessment of DNA damage in postmenopausal women under hormone replacement therapy. Maturitas 2005;51:280-285.
  • Hundal BS, Dhillon VS, Sidhu IS. Genotoxic potential of estrogens. Mut Res 1997;389:173-181.

Dokuz comet parametresine göre kadın ve erkek gruplarda sigara içiminin DNA hasarına etkisi

Year 2012, Volume: 65 Issue: 1, 39 - 46, 01.01.2012
https://doi.org/10.1501/Tipfak_0000000805

Abstract

Amaç: Sigara kullanımı, halk sağlığı için ciddi bir tehdit olușturmaktadır. Çalıșmamızın amacı,
sigara içimi ile lenfosit hücrelerinde DNA hasarı arasındaki ilișkiyi araștırmaktır. Sigaranın potansiyel
genotoksik etkisi, “comet length” (CL), “comet intensity” (CI), “head length” (HL), “head
intensity” (HI), “tail length” (TL), “tail intensity” (TI), “DNA tail” (DNAt), “tail moment” (TM) ve
olive tail moment (OTM) gibi dokuz “comet assay” parametresi ile analiz edilmiștir. İlk kez bu
çalıșmada sigara içenler kadın ve erkek olarak gruplara ayrılmıș ve dokuz comet parametresine
göre değerlendirme yapılmıștır.
Materyal ve Metod: 120 gönüllü birey (60 sigara içmeyen, 60 sigara içen) kan lenfosit hücrelerindeki
DNA hasarları açısından izlenmiștir. DNA hasar dereceleri BAB Bs Comet Assay sistemi ile
ölçülmüștür.
Bulgular: Sigara içen ve içmeyen gruplar arasında CI, TL ve OTM comet parametreleri açısından
yüksek derecede anlamlı ilișki bulundu (p < 0.01). Sigara içen kadın grubunun DNA hasarı açısından
CL, CI, HL, HI, TL, TI (p < 0.01) ve TM (p < 0.05) parametreleri sigara içmeyen kadın grubuna
kıyasla daha yüksektir. Buna karșın, sigara içen ve içmeyen erkek grupları arasında sadece DNAt
ve OTM parametreleri açısından istatistiksel anlamlı fark gözlenmiștir (p < 0.05). Kadınlardan
alınan tüm kan örneklerinin sigara indeksi (SI), tüm çalıșılan comet parametrelerine dayanarak
karșılaștırıldığında, TM dıșındaki bütün parametreler ile istatistiksel olarak anlamlı ilișki bulunmuștur.
Diğer taraftan, erkek grubundaki kan örneklerinde CL, HL, HI, TI ve OTM parametrelerinde
istatistiksel anlamlılık gözlenmiștir (p < 0.05).
Sonuç: Netice olarak, sigaranın DNA hasarlarına sebep olduğu ve kadınların, sigaranın zararlı
etkilerine karșı daha duyarlı olduğu söylenilebilir.

References

  • Church DF, Pryor WA. Free radical chemistry of cigarette smoke and it is toxicological Health Perspect 1985;64:111–126. Environ
  • Li W, Zhou J, Chen L, et al. Lysyloxidase, a critical intra- and extra-cellular target in the pathogenesis. Int J Environ Res Public Health 2011;8:161-184 smoke
  • International Early Lung Tumor Action Investigators
  • susceptibility Women's
  • carcinogens and survival after diagnosis of lung 96:180-184. (IELCAPI). to tobacco tumor. JAMA 2006;
  • American Tumor Society (ACS). Cancer facts and figures. American Tumor Society Atlanta. http://www.cancer.org/ Research/CancerFactsFigures/index 2010
  • Peto R, Lopez AD, Boreham J, et al. Mortality from tobacco in developed countries: indirect estimation from national 1992;339:1268-1278. Lancet
  • Halliwell B, Gutteridge JM. Free radicals and antioxidant protection: mechanisms and significance in toxicology and disease. Hum Toxicol 1988;7:7-13.
  • Mozaffarieh M, Konieczka K, Hauenstein D, et al. Half a pack of cigarettes a day more than doubles DNA breaks in circulating leukocytes. Tobacco Induced Diseases 2010;8:14.
  • Evans MD, Dizdaroglu M, Cooke MS. 2004. Oxidative DNA damage and disease: induction, repair and significance. Mutat Res 567:1–61.
  • Xiong P, Hu Z, Li C, et al. In vitro benzo[a]pyrenediol DNA aberrations in primary lymphocytes, smoking, and risk of squamous cell carcinoma of the head and neck. Int J Cancer 2007;121:2735-2740.
  • Hunt JD, Van der Hel OL, McMillan GP, et al. Renal cell carcinoma in relation to cigarette smoking: meta-analysis of 24 studies. Int J Cancer 2005;114:101-108.
  • Poli P, Buschini A, Spaggiari A, et al. DNA damage by tobacco smoke and some antiblastic drugs evaluated using the comet assay. Toxicol Lett 1999;108:267- 276.
  • Collins A, Cadet J, Epe B, et al. Problems in the measurement of 8-oxoguanine in human DNA. Report of a workshop, DNA oxidation, held in Aberdeen Carcinogenesis 1997;18:1833-1836.
  • Tice RR, Agurell E, Anderson D, et al. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 2000;35: 206–221.
  • Hoffmann H, Hogel J, Speit G. The effect of smoking on DNA effects in the comet assay: a meta-analysis. Mutagenesis 2005;20:455-466.
  • Faust F, KassieF, Knasmuller S, et al. The use of the alkaline comet assay with lymphocytes in human biomonitoring studies. Mutat Res 2004;566:209–229.
  • Singh NP, McCoy MT, Tice RR, et al. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988;175:184–191.
  • Hartmann A, Agurell E, Beevers C, et al. Recommendations for conducting the in vivo alkaline Comet assay. Mutagenesis 2003;18:45–51.
  • El-Zein RA, Monroy CM, Cortes A, et al. Rapid method for determination of DNA repairs capacity in human peripheral blood lymphocytes amongst smokers. BMC Cancer 2010;10:439.
  • Speit G, Witton-Davies T, Heepchantree W, et al. Investigations on the effect of cigarette smoking in the comet assay. Mu- tat Res 2003;542:33-42.
  • Hecht SS. Tobacco smoke carcinogens and lung cancer. J Natl Cancer Inst 1999;91:1194–1210.
  • Stabbert R, Voncken P, Rustemeier K, et al. Toxicological evaluation of an electrically heated cigarette. Part 2: chemical composition of mainstream smoke. J Appl Toxicol 2003;23:329–339.
  • Ferger B, Spratt C, Earl CD, et al. Effects of nicotine on hydroxyl free radical formation in vitro and on MPTP-induced neurotoxicity in vivo. Naunyn- Arch Pharmacol
  • Wetscher GL, Bagchi M, Bagchi D, et al. Free radical production in nicotine treated pancreatic tissue. Free Radic Biol Med 1995;18:877–882.
  • Pandey AK, Bajpayee M, Parmar D, et al. DNA damage in lymphocytes of rural Indian women exposed to biomass fuel smoke as assessed by the Comet assay. Environ Mol Mutagen 2005;45:435-441.
  • Collins AR, Ma AG, Duthie SJ. The kinetics of repair of oxidative DNA damage (strand breaks and oxidized pyrimidines) in human cells. Mutat Res 1995;336:69–77.
  • Hininger I, Chollat-Namy A, Sauvaigo S, et al. Assessment of DNA damage by comet assay on frozen total blood: method and evaluation in smokers and non-smokers. Mutat Res 2004;558: 75-80.
  • DeMarini DM. Genotoxicity of tobacco smoke and tobacco smoke condensate: a review. Mutat Res 2004;567: 447–474
  • Giovannelli L, Saieva C, Masala G, et al. Nutritional and lifestyle determinants of DNA oxidative damage: a study in a Mediterranean genesis 2002;23:1483-1489. Carcino
  • Singh NP, Danner DB, Tice RR, et al. DNA damage and repair with age in individual human lymphocytes. Mutat Res 1990;237:123-130.
  • Singh NP, Danner DB, Tice RR, et al. Basal DNA damage in individual human lymphocytes with age. Mutat Res 1991;256:1–6.
  • Liu X, Yao J, Pisha E, et al. Oxidative DNA damage induced by equine estrogen metabolites: role of estrogen receptor alpha. Chem Res Toxicol 2002;15:512- 519.
  • Ozcagli E, Sardas S, Biri A. Assessment of DNA damage in postmenopausal women under hormone replacement therapy. Maturitas 2005;51:280-285.
  • Hundal BS, Dhillon VS, Sidhu IS. Genotoxic potential of estrogens. Mut Res 1997;389:173-181.
There are 35 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Esma Söylemez This is me

Zeliha Kayaaltı

Vugar Aliyev This is me

Tülin Söylemezoğlu This is me

Publication Date January 1, 2012
Published in Issue Year 2012 Volume: 65 Issue: 1

Cite

APA Söylemez, E., Kayaaltı, Z., Aliyev, V., Söylemezoğlu, T. (2012). Dokuz comet parametresine göre kadın ve erkek gruplarda sigara içiminin DNA hasarına etkisi. Ankara Üniversitesi Tıp Fakültesi Mecmuası, 65(1), 39-46. https://doi.org/10.1501/Tipfak_0000000805
AMA Söylemez E, Kayaaltı Z, Aliyev V, Söylemezoğlu T. Dokuz comet parametresine göre kadın ve erkek gruplarda sigara içiminin DNA hasarına etkisi. Ankara Üniversitesi Tıp Fakültesi Mecmuası. January 2012;65(1):39-46. doi:10.1501/Tipfak_0000000805
Chicago Söylemez, Esma, Zeliha Kayaaltı, Vugar Aliyev, and Tülin Söylemezoğlu. “Dokuz Comet Parametresine göre kadın Ve Erkek Gruplarda Sigara içiminin DNA hasarına Etkisi”. Ankara Üniversitesi Tıp Fakültesi Mecmuası 65, no. 1 (January 2012): 39-46. https://doi.org/10.1501/Tipfak_0000000805.
EndNote Söylemez E, Kayaaltı Z, Aliyev V, Söylemezoğlu T (January 1, 2012) Dokuz comet parametresine göre kadın ve erkek gruplarda sigara içiminin DNA hasarına etkisi. Ankara Üniversitesi Tıp Fakültesi Mecmuası 65 1 39–46.
IEEE E. Söylemez, Z. Kayaaltı, V. Aliyev, and T. Söylemezoğlu, “Dokuz comet parametresine göre kadın ve erkek gruplarda sigara içiminin DNA hasarına etkisi”, Ankara Üniversitesi Tıp Fakültesi Mecmuası, vol. 65, no. 1, pp. 39–46, 2012, doi: 10.1501/Tipfak_0000000805.
ISNAD Söylemez, Esma et al. “Dokuz Comet Parametresine göre kadın Ve Erkek Gruplarda Sigara içiminin DNA hasarına Etkisi”. Ankara Üniversitesi Tıp Fakültesi Mecmuası 65/1 (January 2012), 39-46. https://doi.org/10.1501/Tipfak_0000000805.
JAMA Söylemez E, Kayaaltı Z, Aliyev V, Söylemezoğlu T. Dokuz comet parametresine göre kadın ve erkek gruplarda sigara içiminin DNA hasarına etkisi. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 2012;65:39–46.
MLA Söylemez, Esma et al. “Dokuz Comet Parametresine göre kadın Ve Erkek Gruplarda Sigara içiminin DNA hasarına Etkisi”. Ankara Üniversitesi Tıp Fakültesi Mecmuası, vol. 65, no. 1, 2012, pp. 39-46, doi:10.1501/Tipfak_0000000805.
Vancouver Söylemez E, Kayaaltı Z, Aliyev V, Söylemezoğlu T. Dokuz comet parametresine göre kadın ve erkek gruplarda sigara içiminin DNA hasarına etkisi. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 2012;65(1):39-46.