Investigation of Cytotoxic Effects due to Antibiotic Containing Teicoplanin Active Ingredient on Human Pancreatic Cells by MTT Test

Year 2023, Volume: 76 Issue: 4, 323 - 327, 05.04.2024

Özlem Özdemir

Abstract

Objectives: Antibiotics are a group of drugs used in the treatment of infectious diseases caused by bacteria. They provide powerful treatment against infections, but they are not without side effects. These side effects are not always clinically detectable and their damage at the cellular level may contribute to the development of new disorders. Teicoplanin is one of the glycopeptide antibiotics and is used as a potent agent in the treatment of serious infections caused by Gram-positive bacteria. It may have side effects at cellular level.

Materials and Methods: In this study, the cytotoxicity of the antibiotic teicoplanin in human pancreatic cell (hTERT-HPNE) was investigated. Cytotoxicity was determined using the MTT test. hTERT-HPNE cells were exposed to drug containing the active ingredient teicoplanin. The experimental group concentration was prepared in the range of 0.054 mM, 0.109 mM, 0.218 mM, 0.437 mM, 0.875 mM, 1.75 mM, 3.5 mM, 7 mM.

Results: The active ingredient teicoplanin caused a change in the morphology of the cells in the MTT test. Dose-related decreases in cell viability were observed with the effect of teicoplanin and they were found to be statistically significant compared to the negative control (p<0.05). As a result of the experiment, it was determined that teicoplanin caused cytotoxicity on hTERT-HPNE.

Conclusion: Our study supports the hypothesis that teicoplanin-based antibiotic may have cytotoxic effects on human pancreatic cells and draws attention to the side effects of antibiotics at the cellular level.

Key Words: Teicoplanin, Glycopeptide Antibiotic, MTT Test, Human Pancreatic Cell

Keywords

Teicoplanin , Glycopeptide Antibiotic , MTT Test , Human Pancreatic Cell

Ethical Statement

This study was carried out using a cell line. It does not include human and animal testing. Therefore, ethical committee approval is not required.

Supporting Institution

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Project Number

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Thanks

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