Preventing Testicular Damage in Genital Burns With Cooling: An Experimental Study

Year 2014, Volume: 67 Issue: 1, 33 - 36, 18.03.2015

Ayşe Ebru Abalı

Abstract

Aim: Investigate changes in testes after cooling scrotal burns with melaleuca hydrogel after burn trauma.

Materials and Methods: Twenty male Sprague-Dawley rats were divided into 4 groups of 10 testes each. Five animals in the sham group were anesthetized; both testes were removed through a lower transverse abdominal incision. In the burn-induced groups, a tin container filled with boiling water was used to induce burns. In group T30, scrota were fixed in a hole in a 25×30 cm porcelain wall tile plate and contacted the steam for 30 seconds; in groups T60 and TB60 (melaleuca hydrogel application group), the same procedure was done for 60 seconds. Excisional biopsies of the testes in all groups were done 1 hour later. Proliferation indexes for spermatogenic cells were measured using a PCNA labeling index. Apoptotic activity in spermatogenic cells was measured using the TUNEL index. Epidermal and dermal damage to the skin was measured semiquantitatively.

Results: No significant differences were found regarding proliferative indexes in any group. Increased apoptotic activity was observed in the T30 group, testicular degeneration began with a high apoptotic rate in spermatogenic cells at 60 seconds (P<.05). Application of melaleuca hydrogel to the scrotum after 60- second steam-burn trauma decreased apoptotic activity levels to those of the T30 group (immunehistochemistry) (P>.05). No degeneration of the spermatogenic cells was seen in the TB60 group.

Conclusions: Immediate cooling by applying melaleuca hydrogel after burn trauma decreases the amount of testicular damage in a rat model of experimental scrotal steam burns.

Keywords

Burns , Genital , Cooling , Testis , Experimental , Melaleuca hydrogel

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Genital Yanıklara Bağlı Testis Hasarlanmasına Soğutma Tedavisinin Etkileri: Deneysel Bir Çalışma

Abstract

Amaç: Bu çalışmanın amacı yanık sonrası akut dönemde soğutma ve analjezi amacıyla kullanılan malelauca hidrogel uygulamasının genital bölge yanıklarında testis hasarlanması üzerine etkilerini araştırmaktır.

Gereç ve Yöntem: Yirmi adet erkek spraque-dawley sıçan (250 ± 20gr) 4 gruba ayrıldı. 5 denekten oluşan' 'sham' grubu'nda anestezi altında alçak transvers karın kesisi ile girilerek her iki testis gövde dışına alındı. Bir saat bekletildi. Yanık grupları için kaynar su içeren bir kap kullanıldı. Her bir denek anestezi altında fayans bir plaka üzerine yerleştirildi, fayans plaka üzerinde oluşturulmuş olan deliğin içine her iki testis skrotumile birlikte sabitlendi. T30 grubu kaynar su buharına 30 saniye, T60 grubu kaynar su buharına 60 saniye maruz bırakıldı. Yanık sonrası malelauca hidrojel uygulanan TB60 grubu ise 60 saniye süreyle buharla temas ettirildi. İşlemlerden bir saat sonra testisler eksize edildi. Biyopsi materyali immune-histokimyasal, histolojik çalışmalar için hazırlandı. Testislerdeki apoptotik aktivite için TUNEL indeksi, scrotal derideki epidermal ve dermal hasarlanma için semi-kantitatif yöntem kullanıldı.

Bulgular: Sham grubu ile karşılaştırıldığında T30 grubunda spermatogenik seri, Leydig ve Sertoli hücrelerinde artmış apoptotic aktivite saptandı(p<0.05). T60 grubunda apopitoz artışına ek olarak testis dokusunda dejenerasyon bulguları görüldü. TB60 grubunda ise apopitoz düzeyinin T30 grubundakiyle eş düzeyde olduğu ve dejenerasyona rastlanmadığı gözlemlendi (p>0.05).

Sonuç: Sonuçlarımıza göre, deneysel buhar yanığı modeliyle oluşturulan skrotal yanık sonrası, akut dönemde malelauca hidrojel uygulaması yoluyla soğutmanın, testis dokusundaki hasarlanmayı azaltmaktadır.

Keywords

Yan􀃖k , Genital , So􀃾utma , Testis , Malaleuca hidrojel

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