Histological and Histochemical Examination of Cat and Mouse Small Intestinal Mucosa

Year 1986, Volume: 39 Issue: 2, 105 - 120, 30.06.1986

Cengiz Baycu

Abstract

Histological and Histochemical Stüdy of the Mucosa of Small Intestine of Cat and Mouse İn this stüdy the mucosa of the small intestine of cat and mouse including some exocrine and endocrine celis have been examined under the light microscope in a comparative view. Surface mucous coat and the thickness of the muscularis mucosa were the most striking differences in cat sınail intestine with respect to mouse. The striated border of the small intestine was coated Muþozaszrun 119
with a conspicuous layer of a surface coat which was prominent on the absortive and calciform cells. This coat was intensely PAS-positive (periodic acid-Schiff) when compared to the underlying striated border and it showed uniform coating in all regions. It is concluded that the external coat is a product of the mucous cell itself.
Among the species Paneth cells were seen only in the mouse in the crypts of Lieberkühn. These cells are filled with closely packed secretory granules which were morphologically heterogeneous. They were located in the apical parts of the cells and the content of these granules has been histochemically demonstrated with several methods. The granules consisted of an outer rim or halo which surrounded the central core of the granule. After buffered HgC12 fixation Alcian blue and PAS methods indicated acid mucosubstance and neutral mucopolysaccharide in the rims and it was suggested the presence of neutral mucopolysaccharide and strong basic protein in the cores with PAS and Biebrich scarlet. Large numbers and intensely stained Paneth cells were found mostly in the distal parts of the small intestine. Mitosis has not been observed in Paneth cells. Endocrine cells were abundant in the mucosa of both species. Theqe cells displayed intensely stained basal argentaffin and argyrophi 19 granules in their cytoplasms with silver impregnation methods. En docrine cells were numerous in duodenum but not in Brunner's glands. They usually appeared to be pryramidal in shape and there wasn't any noticeable differences in their morphology and staining intensities between the cells of the species.

Keywords

Paneth , histokimyasal , ekzokrin

Ethical Statement

-

Supporting Institution

-

Project Number

-

Thanks

-

Kedi Ve Fare İnce Bağırsak Mukozasının Histolojik Ve Histokimyasal İncelenmesi

Abstract

Bu çalışmada kedi ve fare ince bağırsak mukozası ile burada yer alan bazı ekzokrin ve endokrin hücreler özel tespit ve boyamalarla ışık mikroskobu düzeyinde karşılaştırmalı olarak incelendi. Dokunun genel histolojik görünümü için HE. ve Azan, histokimyasal içeriğinin belirlenmesinde ise PAS, Alsiyan mavisi boyamaları uygulandı. Farede görülen Paneth hücreleri için de bir seri inceleme yapıldı. Çalışmalarımızda özellikle kedide müşküler tabakanın daha kalın ve yüzey mukus tabakasının iyi gelişmiş olması, kedinin karnivor bir tür olmasından kaynaklanabileceği kanısına varıldı. İki tür arasında hücresel yönden de bazı farklar görüldü; yalnız farede yer alan Paneth hücreleri bu iki türe önemli bir fark getiriyordu. İnce bağırsak dokusunun histokimyasal içeriği ayrı bir önem taşımaktadır bu nedenle çalışmamızda çeşitli yapılar PAS, Alsiyan mavisi ve Biebricll scarleL boyamaları ile ayrı ayrı değerlendirildi, PAS yöntemiyle kedide çizgili kenara yapışık olarak duran yüzey mukus örtüsü'nün devamlı bir tabaka oluşturduğu görüldü Bu yapının hücre membram ile sıkı ilişkide olan bir yapı olduğu kanısına varıldı. Faredeki Paneth hücreleri de bu yöntemle speifik olarak boyanıyordu. Hücrenin salgı granüllerinde çevrede koyu ve merkezi açık boyanan bölgeler tespit edildi. Alsiyan mavisi boyamasında dokuda nötral mukopoli sakkaritlerden başka yoğun bir asit grubunun da yer aldığı görüldü. Bundan başka Paneth hücrelerinde bazik proteinlerin bulunduğu Biebrich scarlet yöntemiyle belirlendi, bu boya ile granüller homojen renkte olup çevredeki halka yapısı görülmüyordu. İnce bağırsak mukozasında endokrin veya enterokromaffin hücreleri her iki türde de yoğun olarak tespit ettik. En fazla duodenumda görülen bu hücreler arjentaffin ve arjirofil granüller içeriyordu. Sonuç olarak elde edilen bulgular çeşitli kaynakların verileri de göz önüne alınarak tartışıldı ve bunları destekler nitelikte olduğu görüldü,

Keywords

Paneth , histokimyasal , ekzokrin

Ethical Statement

-

Supporting Institution

-

Project Number

-

Thanks

-

References

• 1. Behnke, O, Moe, H. : The electron microscope study of mature and differentiating Paneth cells in the rat, especially of their endoplasmic reticulum and lysosomes. J. Cell Biol., 22 : 633, 1964.
• 2. Brown, L.A. : Microvilli of the human jejunal epithelial cell. J. Cell Biol., 12-1, 3 : 623, 1962.
• 3. Cheng, H. Origin, differentiating and renewal of four main epithelial cell types in the mouse small intestine$ IV - Paneth cells. Am. J. Anat., 141-4 : 521, 1974.
• 4. Drury, R.A.B., Wallington, E.A. Carieton's Histological Technique. Oxford University Press N.Y., London, 1967.
• 5. Ferreira, M.N. : Argentaffin and other "Endocrine Cells" of the Small intestine in the adult mouse. I-Ultrastructure and Classification. Am. J. Anat., 131 : 315, 1971.
• 6. Friend, D. : The fine structure of Brunner's glands in the mouse. J. Cell Biol., 25 : 563, 1965.
• 7, Goldfisher, S., Essner, E., Navikoff, AB. : The localization of phosphatase activities at the level of ultrastructure. J. Histochem. and Cytochem., 12 : 72, 1964.
• 8. Hampton, J.S. Effects of fixation on the morphology of the Paneth cell granules. Stain Technology, 40 : 283, 1965.
• 9. Ito, S. : The enteric surface coat on cat intestinal microvilli. J. Cell Biol., 27:475, 1965.
• 10. Lane, B, Lane, P., Rhodin, A.G. : The fine structure of the lamina muscularis mucosae. J. Ultrastruct. Res., 10 : 489, 1964.
• Il. Leppi, T.J., Spicer, S.S. ; The histochemistry of mucins in certain primate salivary glands. Am. J. Anat., 118 : 883, 1966.
• 12. Levellyn, L.J., Srlandsen, S.L. : Morphometric analysis of polarized cell. The Paneth cell. J. Histochem. and Cytochem., 27-11, 554, 1979.
• 13. Lillie, R.D. : Histopathological Technic and Practical Histochemistry. Third edition, McGraw-Hill Book co. N.Y., Toronto, London, Sydney, 1965.
• 14. Nichols, B., Chang, H, Leblond, C.P. : Variability of the shape and argentaffinity of the granules in the enteroendocrine cells of the mouse duodenum. J. Histochcm. and Cytochcm„ 22-10 ; 920, 1074.
• 15. Pearse, A.G.E., Coulling, B, Weavers, B., Friesen, S. . The endocrine polypeptide sells of the human stomach, duodenum and jejunum. Gut, Il : 649, 1970.
• 16, Shean D, Jervis, H.L. : Comparative histochemistry of gastrointestinal muco• substances. Am. J. Anat., 146 : 103, 1976.
• 17. Singh, I. : A new argyrophile method for the rapid staining of enterochromaffin cells in paraffin section. Acta Anat., 59 ;290, 1964.
• 18. Singh, I. : Argyrophile and argentaffin reactions in individual granules of enterochromaffin cells of reserpine treated guinea pigs. Z. Zellforsch., 8 : 501, 1967.
• 19. Speece, A.J. : Histochemichal distribution lysozyme activity in organ of nor mal mice and radiation chimeras. J. Histochem. and Cytochem., 8 : 18, 1960.
• 20. Spicer, S.S. : A correlative study of the histochemichal properties of rodent acid mucopolysaccharides. J. Histochem. and Cytochem. 8 1960.
• 21. Spicer, S.S., Leppi, T.J., Stevard, P.J.H. : Suggest for a histochemichal terminology of carbohydrate-rich tissue components. J. Histochem. and Cytochem. 13-7: 599, 1965.
• 22. Spicer, S.S., Staley, M.W„ Wetzel, BK. : Acid mucosubstance and basic protein in mouse Paneth cells. J. Histochem. and Cytochem. 15-4 : 225, 1967.
• 23. Staley, M.W., Trier, J.S. : Morphologic heterogeneity of mouse Paneth cell gra nules before and after secretory stimulation. Am. J. Anat., 117 : 365, 1965.
• 24. Toner. P. : Fine structure of argyrophile and argentaffin cells in the gastrointestinal tract of the fowl. Z. Zellforsch. 63 : 830, 1964.
• 25. Vander, A.J., Sherman, J.H., Luciano, D.S. : Human Physiology. The mechanisms of body function. Third edition, McGraw-Hill Book Co. London, N.Y. 1980.