House dust specific IgE and basophil degranulation test in house dust sensilive bronchial asthma patients

Year 1985, Volume: 38 Issue: 2, 85 - 94, 30.06.1985

Zeynep Mısırlıgil

Abstract

Skin and basophil degranulation test to house dust by modified Shelley test and total and house dust specific IgE by ELISA were determined in 20 patients with allergic bronchial asthma complained from house dust and nonatopic healthy controls. Raised IgE levels were found in asthma patients studied. Out of 10 patients with a positive skin test with house dust, 10 had a positive basophil degranulation test, 8 had specific IgE antibodies in the sera. Positive basophil degranulation and specific IgE was identified in only one patients showed negative skin test to house dust allergen. It was discussed that basophil degranulation test can be used because of a simple, reliable and practical invitro technique for studying reaginic allergies.

Keywords

basophil , specific IgE , degranulation , allergies

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HASTALARDA SPESIFIK IgE VE BAZOFIL DEGRANULASYON TESTİ

Abstract

Ev tozuna duyarlılık tanımlıyan allerjik bronş astmalı 20 hasta ve atopik olmayan, sağlıklı 10 kişide ev tozu antijeni ile deri testi, modifiye Shelley testi ile bazofil degranülasyon testi, ELISA yöntemi ile total ve evtozuna spesifik IgE araştırıldı. Deri testi pozitif olan 10 astmalı hastanin hepsinde bazofil degraniilasyonu pozitif, 8'inde spesifik IgE bulundu. Deri testi negatif bir hastada, bazofil degraniilasyonu ve spesifik IgE pozitif olarak bulundu. Astmali hasta grubunda total IgE yüksek degerlerde saptandi. Allergenlerin saptanmasmda bazofil degrantilasyon testinin invitro, basit, giivenilir ve pratik bir yöntem olmasi açısından uygulanabilirligi tartisildi.

Keywords

Allergen , bronş , IgE , deri testi

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Supporting Institution

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Project Number

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Thanks

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