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Otolog Serum Substrafı ile Kanda ve İdrarda Pepsinojen Aktivitesi Tayin Üzerine Bir Çalışma

Year 1974, Volume: 27 Issue: 1-2, 106 - 118, 30.06.1974

Necip Danişoğlu

Abstract

Gastrointestinal bir hastalık belirtisi göstermeyen 21 kişilik normaller grubu ile 19 duodenal ülserli ve 8 aşilili vak'ada otolog serum substratı kullanılarak kan ve idrarda pepsinojen aktivite tayinleri yapılmıştır. Ayrıca bu grupların mide asiditeleri incelenmiştir. Kanda ve idrarda aktivite, poteoliz sonucu açığa çıkan tirozin olarak (mg, moWml/24 saat) m.I.U. cinsinden hesaplanmıştır. Ortalama standart hata hesabıyla normaller için kanda 270 + 57, idrarda 1536 387, duodenum ülserli hastalar grubunda sırayla 260 + 72 ve 1659 662, asidi gösterenlerde ise 174 + 44 ve 1454 500 bulunmuştur. İstatistik önem bakımından ortalamalar arasında farkların bu gruplarda önemsiz olduğu saptanmıştır.

Keywords

pepsinojen enzim serum substrafı

References

  • 1 Michaelis L. : Die Wirkungsbeaingungen des Pepsin Biochem. Z. 65:1, (1914).
  • 2 Northrop J. H. : Pepsin activity units and methods for determining peptic activity. J. Gen. Physiol. 5:263, (1922).
  • 3 Gottlieb. S. : Untersuchungen uber die Pepsinmengen in Blut und Harn. Skand. Arch. Physiol. 46:1, (1924).
  • 4 Anson M. L. and mirsky A. E. Estimation of pepsin with hemoglobin. J. Gen. Physiol. 16:59, (1932).
  • 5 West P. M. : Simplified method for ûetermining excretion rate of uropepsin. J. Lab. Clin. Med. 39: 159, (1952).
  • 6 Goodman, R. D. : Observation of som of technical and Clinical factors influencing determining of uropepsin excretion in man, J. Lab. Clin. Med. 40:872, (1952).
  • Edwards, K. : Value of plasma pepsjnojen estimation. Brit. Med. J. 5165:30, (1960).
  • 8 Chiang, L. : The separate determination of human pepsin and gastricin. Proc. soc, Exptl. Biol. Med. 122:700, (1966).
  • 9 — Klotz, A. P. : The laboratory determination of pepsin in gastric juice with radioactive iodinated albumin. J. Lab. Clin. Med. 50: 753, (1957).
  • 10 — Uete, T. , Wasa Mr. : A simplified method for the determination of pepsinojen in blood and urine. Clin. Chem. 15 (1) 42, (1969).
  • 11 — Van Slyke : The Estimation of plasma protein concentration from plasma specific gravity. J. Biol. Chem. 183 :349, (1950)
  • 12 — Varley, H. : Pratical Clinical Biochemistry Third edition, s. 187 ve 381. William Heinemann Medical Books Ltd. London, (1963).
  • 13 — Mirsky, A. E. ; Blood plasma pepsinogen. I The source, properties and assay of the proteolytic of plasma at acid reactions. J. Lab. Clin. Med. 40:17, (1952).
  • 14 — Lombarts. A., J. P. F. and Peters, H. J. : Routine determination of serum pepsinogen. Clin. Chim. Acta. 36:195, (1972).
  • 15 — Sandifer, M. G. : Serum pepsinogen determinetion in hospitalized alcoholic patients. Quart. J. Studies AIC. 22 (1), 27 - 33, (1961). Biol. Abst. 36 (15) No. 48067, (1961).
  • 16 — Card, W. I. : The relationship between acid output of the stomach following maximal histamine stimulation and the parietal cell. mass. Clin. sci. 19:147, (1960).
  • 17 — Hanley, W. B. : Hereditary aspect of duodenal ulceration. Brit. Med. J. 1•.936, (1964).
  • 18 — A. R. : Plasma pepsinogen, normal and abnormal secretion, Ann. Roy. coll. surg. Engl. 35:34, (1964).
  • 19 — Küley, M. : Mide fonksiyonlarlmn incelenmesinde üropepsinojenin degeri. Türkiye TIP Akademisi Mecmuasl, 6 -4 801 (1971 h 20 — Vickers, H. E. : Diagnostik Value of a Single Plasma Pepsinogen Estimetion. Brit. Med. J. 1453, (1962).
  • 21 — Savoye, B. : Valeur du pepsinogéne sérique dans les diagnostic des affections gastroduodénales. Arch. Fr. Mal. Dig. 58: 719, (1969).
  • 22 — Ronski, R. : Serum pepsinogen in cirrhosis of the liver. Federation Pro (Translation Suppl.) 22 (3) :540, (1963).
  • 23 — Hirschowitz, B. I. : Pepsinogen : Its origins, secretion and excretion. Physiol. Rev. 37:475, (1957).

Determinaticn of the Activity of Pepsinojen in Blood and Urine

Year 1974, Volume: 27 Issue: 1-2, 106 - 118, 30.06.1974

Necip Danişoğlu

Abstract

Determinaticn of the Activity of Pepsinojen in Blood and Urine
autologous serum substrate, determination of the activity cf pLpsinojen in blood and urine is performed among a grcup of' 21 normal people showing no gastrointestinal symptom, 19 patients suffering from duodenal ulcer and 8 achilia. Gastric acidity of these groups is also investigated. Activities in blood and urine are calculated as the m.I.U. of tyrosine (mg mol/ml/24 hr) liberated after the proteolysis.
After the standard error calculation, average value for the normal is found as 270 ± 57 in blood and 1536 + 387 in urine, the blood and urine values for duodenal ulcer are 260 + 72 and 1659 ± 662 respectively.
It is demonstrated that the differences between the averages of the groups has no significance from statistical point of view.

Keywords

enzyme substraf of serum pepsinojen

Supporting Institution

Ankara University Faculty of Medicine

References

  • 1 Michaelis L. : Die Wirkungsbeaingungen des Pepsin Biochem. Z. 65:1, (1914).
  • 2 Northrop J. H. : Pepsin activity units and methods for determining peptic activity. J. Gen. Physiol. 5:263, (1922).
  • 3 Gottlieb. S. : Untersuchungen uber die Pepsinmengen in Blut und Harn. Skand. Arch. Physiol. 46:1, (1924).
  • 4 Anson M. L. and mirsky A. E. Estimation of pepsin with hemoglobin. J. Gen. Physiol. 16:59, (1932).
  • 5 West P. M. : Simplified method for ûetermining excretion rate of uropepsin. J. Lab. Clin. Med. 39: 159, (1952).
  • 6 Goodman, R. D. : Observation of som of technical and Clinical factors influencing determining of uropepsin excretion in man, J. Lab. Clin. Med. 40:872, (1952).
  • Edwards, K. : Value of plasma pepsjnojen estimation. Brit. Med. J. 5165:30, (1960).
  • 8 Chiang, L. : The separate determination of human pepsin and gastricin. Proc. soc, Exptl. Biol. Med. 122:700, (1966).
  • 9 — Klotz, A. P. : The laboratory determination of pepsin in gastric juice with radioactive iodinated albumin. J. Lab. Clin. Med. 50: 753, (1957).
  • 10 — Uete, T. , Wasa Mr. : A simplified method for the determination of pepsinojen in blood and urine. Clin. Chem. 15 (1) 42, (1969).
  • 11 — Van Slyke : The Estimation of plasma protein concentration from plasma specific gravity. J. Biol. Chem. 183 :349, (1950)
  • 12 — Varley, H. : Pratical Clinical Biochemistry Third edition, s. 187 ve 381. William Heinemann Medical Books Ltd. London, (1963).
  • 13 — Mirsky, A. E. ; Blood plasma pepsinogen. I The source, properties and assay of the proteolytic of plasma at acid reactions. J. Lab. Clin. Med. 40:17, (1952).
  • 14 — Lombarts. A., J. P. F. and Peters, H. J. : Routine determination of serum pepsinogen. Clin. Chim. Acta. 36:195, (1972).
  • 15 — Sandifer, M. G. : Serum pepsinogen determinetion in hospitalized alcoholic patients. Quart. J. Studies AIC. 22 (1), 27 - 33, (1961). Biol. Abst. 36 (15) No. 48067, (1961).
  • 16 — Card, W. I. : The relationship between acid output of the stomach following maximal histamine stimulation and the parietal cell. mass. Clin. sci. 19:147, (1960).
  • 17 — Hanley, W. B. : Hereditary aspect of duodenal ulceration. Brit. Med. J. 1•.936, (1964).
  • 18 — A. R. : Plasma pepsinogen, normal and abnormal secretion, Ann. Roy. coll. surg. Engl. 35:34, (1964).
  • 19 — Küley, M. : Mide fonksiyonlarlmn incelenmesinde üropepsinojenin degeri. Türkiye TIP Akademisi Mecmuasl, 6 -4 801 (1971 h 20 — Vickers, H. E. : Diagnostik Value of a Single Plasma Pepsinogen Estimetion. Brit. Med. J. 1453, (1962).
  • 21 — Savoye, B. : Valeur du pepsinogéne sérique dans les diagnostic des affections gastroduodénales. Arch. Fr. Mal. Dig. 58: 719, (1969).
  • 22 — Ronski, R. : Serum pepsinogen in cirrhosis of the liver. Federation Pro (Translation Suppl.) 22 (3) :540, (1963).
  • 23 — Hirschowitz, B. I. : Pepsinogen : Its origins, secretion and excretion. Physiol. Rev. 37:475, (1957).
There are 22 citations in total.

Details

Primary Language Turkish
Subjects Gastroenterology and Hepatology, Medical Biochemistry - Proteins, Peptides and Proteomics
Journal Section Articles
Authors

Necip Danişoğlu This is me

Publication Date June 30, 1974
Published in Issue Year 1974 Volume: 27 Issue: 1-2

Cite

APA Danişoğlu, N. (1974). Otolog Serum Substrafı ile Kanda ve İdrarda Pepsinojen Aktivitesi Tayin Üzerine Bir Çalışma. Ankara Üniversitesi Tıp Fakültesi Mecmuası, 27(1-2), 106-118.
AMA Danişoğlu N. Otolog Serum Substrafı ile Kanda ve İdrarda Pepsinojen Aktivitesi Tayin Üzerine Bir Çalışma. Ankara Üniversitesi Tıp Fakültesi Mecmuası. June 1974;27(1-2):106-118.
Chicago Danişoğlu, Necip. “Otolog Serum Substrafı Ile Kanda Ve İdrarda Pepsinojen Aktivitesi Tayin Üzerine Bir Çalışma”. Ankara Üniversitesi Tıp Fakültesi Mecmuası 27, no. 1-2 (June 1974): 106-18.
EndNote Danişoğlu N (June 1, 1974) Otolog Serum Substrafı ile Kanda ve İdrarda Pepsinojen Aktivitesi Tayin Üzerine Bir Çalışma. Ankara Üniversitesi Tıp Fakültesi Mecmuası 27 1-2 106–118.
IEEE N. Danişoğlu, “Otolog Serum Substrafı ile Kanda ve İdrarda Pepsinojen Aktivitesi Tayin Üzerine Bir Çalışma”, Ankara Üniversitesi Tıp Fakültesi Mecmuası, vol. 27, no. 1-2, pp. 106–118, 1974.
ISNAD Danişoğlu, Necip. “Otolog Serum Substrafı Ile Kanda Ve İdrarda Pepsinojen Aktivitesi Tayin Üzerine Bir Çalışma”. Ankara Üniversitesi Tıp Fakültesi Mecmuası 27/1-2 (June1974), 106-118.
JAMA Danişoğlu N. Otolog Serum Substrafı ile Kanda ve İdrarda Pepsinojen Aktivitesi Tayin Üzerine Bir Çalışma. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 1974;27:106–118.
MLA Danişoğlu, Necip. “Otolog Serum Substrafı Ile Kanda Ve İdrarda Pepsinojen Aktivitesi Tayin Üzerine Bir Çalışma”. Ankara Üniversitesi Tıp Fakültesi Mecmuası, vol. 27, no. 1-2, 1974, pp. 106-18.
Vancouver Danişoğlu N. Otolog Serum Substrafı ile Kanda ve İdrarda Pepsinojen Aktivitesi Tayin Üzerine Bir Çalışma. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 1974;27(1-2):106-18.