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Study of the Relationship Between Blood Group Compatibility and Certain Erythrocyte Antigens with Blastic Transformation

Year 1971, Volume: 24 Issue: 1, 145 - 165, 28.02.1971

Bekir Sıtkı Şaylı

Abstract

Bu çalışmada birinci dereceden akrabalarla akraba olmıyanlardan yapılan karışık lökosit kültürlerinde lenfositlerin blastik transformasyonunu tayin ve bu reaksiyonla P, Duffy, Kell/Cella• 110, MN ve ss eritrosit antijenleri arasında ilişki bulunup bulun madığını tesbit amacıyla 45 tanesi 1. deveceden akraba 63 kişinin periferik lökositleri kullanıldı, Fitohemagıütinin ihtiva etmiyen standard kültürlerdeki reaksiyon sitolojik metodlarla değerIendirilclj. Akraba grubunda stimülasyonun karşılıklı olarak meydana gel mediği ve stimiilasyonla söz konusu antijenlerin ilişkisinin bulun, matlığı gösterilerek KIR nun allograft transplantasyonunda histokompatjbiliteyi tayin için test olarak kullanılabileceği belirtildi,
Transformasyonun mekanizmasını açıklamak üzere lenfositlerde özel reaktif kesimlerin varlığım öngören bir hipotez ortaya atıldl.

Keywords

Lenfosit Aktivasyonu Blastik Dönüşüm Kromozom Morfolojisi

References

  • 1— Agarwall, S.S., Bugbee, S.J., Loeb, L. A. : Induction of DNA polymerase in mixed lymphocyte cultures. Nature, 226: 949 1970.
  • 2 — Bach, F.H.: First International Congress of the Transplantation Society (abstr.), s. 172, Paris, 1967. Kasakura ve Lowenstein'da, 1968.
  • 3 - Hirschhorn, K: The in vitro immun response of peripheral blood lyriphocytos. Seminars in Hematology, 2: bb, 1965.
  • 5 — Kisken, W.A.: Transplantation, 5: 1046, 1967. Hardy ve Ling' de, 1969.
  • 6 - Voynow, N.K.: One-way stimulation in mixed leucocyte cul- tures. Science, 153: 545 1966.
  • 7 -Bain, B, Lowenstein, ll.: Genetic studies on the mixed leucocyte reaction. ibid, 145 • . 1315, 1964.
  • 8 -Vas, M.R., Lowenstein, Ja.: The development of large im• mature mononuclear cells in mixed leucocyte cultures. Blood, 23: 108-116, 1964.
  • 9 - Federation Proc., 22: 428 (abstr.), 1963.
  • 10 — Cooperband, S.R., Rosen, F.S., Kibrick, S.: Studies on the in vitro behavior of ogamunaglobulinemic lymphocytes. J. Clin. invest., 47: 836-847, 1968.
  • Il — Dutton, R.W.: Furthei• studies of the stimulation of DNA synthesis in cultures of spleen cell suspensions by homologous cells in inbred strains of mice and rats. J. Exp. Med., 122 : 759 1965. 12 — Elkins, W.L., Palm, J.E.: Identification of a strong histocompatibility locus in the rat by normal spleen cell transfer. Ann. NY. Acad. Sci., 129: 573, 1967.
  • 13 — Elves, M.W.: Comparison of mitomycin C and X-rays for the production of one-way stimulation in mixed leucocyte cultures. Nature, 223: 90, 1969.
  • 14 — Gedda, L., Brenci, G.: The genetic conditioning of the mitotic index and the association index of acrocentric chromosomes. Acta Genet. Med. Gemellol., 19: 179, 1970.
  • 15 — Gell, P.G.H., Sell, S.: Studies on rabbit lymphocytes in vitro. J. Exp. Med., 122: 813, 1965.
  • 16 — Gordon, J., MacLean, L.D.: A lyniphocyte—stimulat.ing factor produced in vitro. Nature, 208: 795, 1965.
  • 17 — Gowans, J.L., Mc Gregor, D.D. : The immunological activities of lymphocytes. Progr. Allergy, 9: 1, 1965.
  • 18 — Graw, R.G„ Herzig, G.P., Rogentine, G.N., J., Yankee, R.A:, Leventhal, B.G., Whang-Peng, J., Halterman, R.H., Krüger, G., Berard, C., Henderson, E.S.: Graft-versus-host reaction complicating HL-A matched bone-marrow transplantation. Lancet, ii: 1053, 1970.
  • 19 — Hardy, D.A., Ling, N.R.: Effects of some cellular antigens on lymphocytes and the nature of the mixed lymphocyte reaction. Nature, 221: 545 1969.
  • 20 Hashem, N., Rosen, F.S.: Mitogenic fractions in human peripheral lymphocyee extracts. Lancet, i: 201, 1964.
  • 21 — Hirschhorn, R., Troll, W., Brittinger, G., Weissmann, G.: Template activity of nuclei from stimulated lymphocytes. Nature, 222: 1247 1969.
  • 22 — Inbar, M., Sachs, L.: Structural difference in sites on the surface membrane of normal and transformed cells. ibid, 223: 710, 1969.
  • 23 Kasakura, S., Lowenstein, L. : Irradiated and preserved leucocytes in mixed leucocyte cultures. Proc. Soc. Exp. Biol. Med., 125: 355, 1967.
  • 24 — The factors affecting the strength of 'one-way' stimulation with irradiated leucocytes in mixed leucocyte cultures. J. immun., 101: 12 1968.
  • 25 — Kisken, W.A., Malek, G.H.: Kidney allograft survival in the dog predicted by mixed leucocyte culture tests. Nature, 224: 1110, 1969.
  • 26 — Swenson, N.A.: Unresponsiveness of mixed leucocyte cultures from thymectomized adult dogs. ibid, s. 76-77.
  • 27 — Kissrneyer-NielLsen, F. , Svejgaard, A., Sorensen, S.F., Nielsen, L.S:, Thorsby, E.: Mixed lymphocyte cultures and I-IL-A identity in unrelated subjects. ibid, 228: 63, 1970.
  • 28 — Ahrons, S., Nielsen, L.S.: Crossingover within the HL-A system. ibid, 224: 75, 1965.
  • 29 — Lowenstein, L, Bain, B.: Blastogenesis and histocompatibility. Vox sang., 11: 305, 1966.
  • 30 — Sell, S., Rowe, D.S., Gell, P.G.H.: Studies on rabbit lymphocytes in vitro. J. Exp. Med., 122: 823, 1965.
  • 31 — Schrek, R., Donnelly, W. J.: Differences between lymphocytes of leukemic and non-leukemic patients with respect to morphologic features, motility and susceptibility to guinea pig serum. Blood, 18: 561, 1961.
  • 32 — Schwarz, M.R.: The mixed lymphocyte reaction: An in vitro test for tolerance. J. Exp. Med., 127: 879, 1968.
  • 33 — Surianu, P., Heiniger, H.J., Feinendegen, L.E.: Verminderte Phytohemaglutinin-Stimulation von menschlichen Lymhozytenkulturen bei Leberzirrhose: Untersuchengen mit •IH-Thymidin und 125J—Desoxyuridin. Blut, XX (6): 370, 1970.
  • 34 — Sayll, B.S.: Secondary constrictions and heteropycnotic segments of human chromosomes. Bull. Johns Hopkins Hosp., 114: 152, 1964.
  • 35 — Blastik transformasyonda serom faktörleri ve heterolog lökositlerin etkileri. A.Ü. TIP Fak. Mec., 22: 749, 1969.
  • 36 — Waiford, R.L., Finkelstein, S., Hanna, C., Collins, Z.: Third sublocus in the HL—A human transplantation system. Nature, 224: 74, 1969.
  • 37 — Wilson, D.B., Silvers, W.K., Nowell, P.C.: Quantitative studies on the mixed lymphocyte interaction in rats. J. Exp. Med., 126 : 655, 1967.

Kan Yakınlığı ve Bazı Eritrosit Antijenlerinin Blastik Dönüşümle İlişkilerinin Araştırılması

Year 1971, Volume: 24 Issue: 1, 145 - 165, 28.02.1971

Bekir Sıtkı Şaylı

Abstract

Investigations on the Relationship between Consanguinity, Blastic Transformation and Some Erytkrocyte Antigens
In this study to measure the degree of transformation of lymphocytes in mixed leucocyte cultures from those who are relatives and those of unrelated individuals and to test if there is any relationship between the erythrocyte antigens of P, Duffy, Kell/Cel• lano, MN and ss, peripheral leucocytes have been taken from 63 individuals, 45 of them are first-degree relatives. Reactions in standard cultures not containing phytohemagglutinin have been evaluated with cytologic techniques. It has been found that no stimulation in those cultures from first-degree relatives occurs and there is no relation between antigens tested so far. To explain the mechanism of lymphocyte transformation it has been postulated the existence of some specific reactive sites on lymphocytes.

Keywords

Lymphocyte Activation Blastic Transformation Chromosome Morphology

References

  • 1— Agarwall, S.S., Bugbee, S.J., Loeb, L. A. : Induction of DNA polymerase in mixed lymphocyte cultures. Nature, 226: 949 1970.
  • 2 — Bach, F.H.: First International Congress of the Transplantation Society (abstr.), s. 172, Paris, 1967. Kasakura ve Lowenstein'da, 1968.
  • 3 - Hirschhorn, K: The in vitro immun response of peripheral blood lyriphocytos. Seminars in Hematology, 2: bb, 1965.
  • 5 — Kisken, W.A.: Transplantation, 5: 1046, 1967. Hardy ve Ling' de, 1969.
  • 6 - Voynow, N.K.: One-way stimulation in mixed leucocyte cul- tures. Science, 153: 545 1966.
  • 7 -Bain, B, Lowenstein, ll.: Genetic studies on the mixed leucocyte reaction. ibid, 145 • . 1315, 1964.
  • 8 -Vas, M.R., Lowenstein, Ja.: The development of large im• mature mononuclear cells in mixed leucocyte cultures. Blood, 23: 108-116, 1964.
  • 9 - Federation Proc., 22: 428 (abstr.), 1963.
  • 10 — Cooperband, S.R., Rosen, F.S., Kibrick, S.: Studies on the in vitro behavior of ogamunaglobulinemic lymphocytes. J. Clin. invest., 47: 836-847, 1968.
  • Il — Dutton, R.W.: Furthei• studies of the stimulation of DNA synthesis in cultures of spleen cell suspensions by homologous cells in inbred strains of mice and rats. J. Exp. Med., 122 : 759 1965. 12 — Elkins, W.L., Palm, J.E.: Identification of a strong histocompatibility locus in the rat by normal spleen cell transfer. Ann. NY. Acad. Sci., 129: 573, 1967.
  • 13 — Elves, M.W.: Comparison of mitomycin C and X-rays for the production of one-way stimulation in mixed leucocyte cultures. Nature, 223: 90, 1969.
  • 14 — Gedda, L., Brenci, G.: The genetic conditioning of the mitotic index and the association index of acrocentric chromosomes. Acta Genet. Med. Gemellol., 19: 179, 1970.
  • 15 — Gell, P.G.H., Sell, S.: Studies on rabbit lymphocytes in vitro. J. Exp. Med., 122: 813, 1965.
  • 16 — Gordon, J., MacLean, L.D.: A lyniphocyte—stimulat.ing factor produced in vitro. Nature, 208: 795, 1965.
  • 17 — Gowans, J.L., Mc Gregor, D.D. : The immunological activities of lymphocytes. Progr. Allergy, 9: 1, 1965.
  • 18 — Graw, R.G„ Herzig, G.P., Rogentine, G.N., J., Yankee, R.A:, Leventhal, B.G., Whang-Peng, J., Halterman, R.H., Krüger, G., Berard, C., Henderson, E.S.: Graft-versus-host reaction complicating HL-A matched bone-marrow transplantation. Lancet, ii: 1053, 1970.
  • 19 — Hardy, D.A., Ling, N.R.: Effects of some cellular antigens on lymphocytes and the nature of the mixed lymphocyte reaction. Nature, 221: 545 1969.
  • 20 Hashem, N., Rosen, F.S.: Mitogenic fractions in human peripheral lymphocyee extracts. Lancet, i: 201, 1964.
  • 21 — Hirschhorn, R., Troll, W., Brittinger, G., Weissmann, G.: Template activity of nuclei from stimulated lymphocytes. Nature, 222: 1247 1969.
  • 22 — Inbar, M., Sachs, L.: Structural difference in sites on the surface membrane of normal and transformed cells. ibid, 223: 710, 1969.
  • 23 Kasakura, S., Lowenstein, L. : Irradiated and preserved leucocytes in mixed leucocyte cultures. Proc. Soc. Exp. Biol. Med., 125: 355, 1967.
  • 24 — The factors affecting the strength of 'one-way' stimulation with irradiated leucocytes in mixed leucocyte cultures. J. immun., 101: 12 1968.
  • 25 — Kisken, W.A., Malek, G.H.: Kidney allograft survival in the dog predicted by mixed leucocyte culture tests. Nature, 224: 1110, 1969.
  • 26 — Swenson, N.A.: Unresponsiveness of mixed leucocyte cultures from thymectomized adult dogs. ibid, s. 76-77.
  • 27 — Kissrneyer-NielLsen, F. , Svejgaard, A., Sorensen, S.F., Nielsen, L.S:, Thorsby, E.: Mixed lymphocyte cultures and I-IL-A identity in unrelated subjects. ibid, 228: 63, 1970.
  • 28 — Ahrons, S., Nielsen, L.S.: Crossingover within the HL-A system. ibid, 224: 75, 1965.
  • 29 — Lowenstein, L, Bain, B.: Blastogenesis and histocompatibility. Vox sang., 11: 305, 1966.
  • 30 — Sell, S., Rowe, D.S., Gell, P.G.H.: Studies on rabbit lymphocytes in vitro. J. Exp. Med., 122: 823, 1965.
  • 31 — Schrek, R., Donnelly, W. J.: Differences between lymphocytes of leukemic and non-leukemic patients with respect to morphologic features, motility and susceptibility to guinea pig serum. Blood, 18: 561, 1961.
  • 32 — Schwarz, M.R.: The mixed lymphocyte reaction: An in vitro test for tolerance. J. Exp. Med., 127: 879, 1968.
  • 33 — Surianu, P., Heiniger, H.J., Feinendegen, L.E.: Verminderte Phytohemaglutinin-Stimulation von menschlichen Lymhozytenkulturen bei Leberzirrhose: Untersuchengen mit •IH-Thymidin und 125J—Desoxyuridin. Blut, XX (6): 370, 1970.
  • 34 — Sayll, B.S.: Secondary constrictions and heteropycnotic segments of human chromosomes. Bull. Johns Hopkins Hosp., 114: 152, 1964.
  • 35 — Blastik transformasyonda serom faktörleri ve heterolog lökositlerin etkileri. A.Ü. TIP Fak. Mec., 22: 749, 1969.
  • 36 — Waiford, R.L., Finkelstein, S., Hanna, C., Collins, Z.: Third sublocus in the HL—A human transplantation system. Nature, 224: 74, 1969.
  • 37 — Wilson, D.B., Silvers, W.K., Nowell, P.C.: Quantitative studies on the mixed lymphocyte interaction in rats. J. Exp. Med., 126 : 655, 1967.
There are 35 citations in total.

Details

Primary Language English
Subjects Medical Genetics (Excl. Cancer Genetics)
Journal Section Articles
Authors

Bekir Sıtkı Şaylı This is me

Publication Date February 28, 1971
Published in Issue Year 1971 Volume: 24 Issue: 1

Cite

APA Şaylı, B. S. (1971). Kan Yakınlığı ve Bazı Eritrosit Antijenlerinin Blastik Dönüşümle İlişkilerinin Araştırılması. Ankara Üniversitesi Tıp Fakültesi Mecmuası, 24(1), 145-165.
AMA Şaylı BS. Kan Yakınlığı ve Bazı Eritrosit Antijenlerinin Blastik Dönüşümle İlişkilerinin Araştırılması. Ankara Üniversitesi Tıp Fakültesi Mecmuası. February 1971;24(1):145-165.
Chicago Şaylı, Bekir Sıtkı. “Kan Yakınlığı Ve Bazı Eritrosit Antijenlerinin Blastik Dönüşümle İlişkilerinin Araştırılması”. Ankara Üniversitesi Tıp Fakültesi Mecmuası 24, no. 1 (February 1971): 145-65.
EndNote Şaylı BS (February 1, 1971) Kan Yakınlığı ve Bazı Eritrosit Antijenlerinin Blastik Dönüşümle İlişkilerinin Araştırılması. Ankara Üniversitesi Tıp Fakültesi Mecmuası 24 1 145–165.
IEEE B. S. Şaylı, “Kan Yakınlığı ve Bazı Eritrosit Antijenlerinin Blastik Dönüşümle İlişkilerinin Araştırılması”, Ankara Üniversitesi Tıp Fakültesi Mecmuası, vol. 24, no. 1, pp. 145–165, 1971.
ISNAD Şaylı, Bekir Sıtkı. “Kan Yakınlığı Ve Bazı Eritrosit Antijenlerinin Blastik Dönüşümle İlişkilerinin Araştırılması”. Ankara Üniversitesi Tıp Fakültesi Mecmuası 24/1 (February 1971), 145-165.
JAMA Şaylı BS. Kan Yakınlığı ve Bazı Eritrosit Antijenlerinin Blastik Dönüşümle İlişkilerinin Araştırılması. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 1971;24:145–165.
MLA Şaylı, Bekir Sıtkı. “Kan Yakınlığı Ve Bazı Eritrosit Antijenlerinin Blastik Dönüşümle İlişkilerinin Araştırılması”. Ankara Üniversitesi Tıp Fakültesi Mecmuası, vol. 24, no. 1, 1971, pp. 145-6.
Vancouver Şaylı BS. Kan Yakınlığı ve Bazı Eritrosit Antijenlerinin Blastik Dönüşümle İlişkilerinin Araştırılması. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 1971;24(1):145-6.