Diagnosis and Determination of Viral Disease Agents in Olive Saplings in Aydin, Balikesir and Izmir Provinces

Abstract

This study was carried out in order to determine the viral agents in the nurseries in the olive growing production areas in Aydın, Balıkesir and İzmir provinces between 2010 and. A total of 120 samples, 40 from each province, were collected from the nurseries in Aydın, Balıkesir and İzmir provinces. Molecular methods were applied to identify viral agents in collected olive samples. Arabis mosaic virus (ArMV), cucumis mosaic virus (CMV), cherry leaf roll virus (CLRV), strawberry latent ringspot nepovirus (SLRSV), olive latent-1 virus (OLV-1), olive latent-2 virus (OLV-2), olive latent-3 virus (OLV-3), olive latent ringspot virus (OLRSV) and olive leaf yellowing-associated virus (OLYaV) searched by RT-PCR molecular method. As a result of molecular analyzes; It was determined that there was 32.5%, 45% and 35% virus infection in Aydın, Balıkesir and İzmir provinces, respectively. It was determined that the collected samples were infected with 27.5% CMV and 7.5% ArMV in İzmir, 20% CMV and 12.5% ArMV in Aydın, 30% CMV and 15% ArMV in Balıkesir. As a result of the analysis, it was revealed that the viruses CLRV, SLRSV, OLV-1, OLV-2, OLRSV, OLYaV and OLV-3 were not found to infected in the samples taken from the nurseries.

Keywords

• Olive
• Virus diseases
• RT-PCR

Aydın, Balıkesir ve İzmir İllerinde Zeytin Fidanlarındaki Viral Hastalık Etmenlerinin Tanılanması ve Varlığının Belirlenmesi

Öz

Bu çalışma 2009 ve 2012 yılları arasında Aydın, Balıkesir ve İzmir illerinde yaygın olarak zeytin yetiştiriciliği yapılan üretim alanlarındaki fidanlıklarda virüs hastalıklarının belirlenmesi, toplanan örneklerde virüslerin bulunma durumlarının ortaya konması amacıyla yürütülmüştür. Aydın, Balıkesir ve İzmir illerindeki fidanlıklardan her ilden 40 adet olmak üzere, toplam 120 adet fidan örneği alınmıştır. Zeytin örneklerindeki viral etmenlerin tanılanması moleküler yöntemler kullanılarak yapılmıştır. Arabis mosaic virus (ArMV), cucumber mosaic virus (CMV), cherry leafroll virus (CLRV) ve strawberry latent ringspot virus (SLRSV), olive latent-1 virus (OLV-1), olive latent-2 virus (OLV-2), olive latent-3 virus (OLV-3), olive latent ringspot virus (OLRSV) ve olive leaf yellowing-associated virus (OLYaV) adlı virüslerin varlığı RT-PCR ile araştırılmıştır. Alınan fidan örneklerinde yapılan analizler sonucunda; Aydın ilinde %32.5, Balıkesir ilinde %45 ve İzmir ilinde %35 oranlarında virüs enfeksiyonu olduğu tespit edilmiştir. Toplanan örneklerin İzmir ilinde %27.5 CMV ve %7.5 ArMV, Aydın ilinde %20 CMV ve %12.5 ArMV, Balıkesir ilinde ise %30 CMV ve %15 oranlarında ArMV ile enfekteli olduğu belirlenmiştir. Fidanlıklardan alınan örneklerde CLRV, SLRSV, OLV-1, OLV-2, OLRSV, OLYaV ve OLV-3 adlı virüslerin enfeksiyonunun olmadığı analizler sonucunda ortaya konmuştur.

Anahtar Kelimeler

• Zeytin
• virüs hastalıkları
• RT-PCR

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