Haşhaş (Papaver somniferum) Kök Boğazı ve Kapsül Yanıklığı Etmeni Alternaria papavericola’nın Moleküler Karakterizasyonu

Abstract

Hem dünyada hem de ülkemizde önemli tıbbi ve aromatik bitkiler arasında yer alan haşhaş (Papaver somniferum L.) bitkisinin tohumlarından ve yağından da gıda olarak yararlanılmaktadır. Bu çalışmada Isparta ilinden toplanan haşhaş tohumlarından izole edilen, haşhaşta kök boğazı ve kapsül yanıklığına neden olan Alternaria papavericola Woudenb. & Crous’nın moleküler karakterizasyonunun yapılması amaçlanmıştır. Bu amaçla rDNA’nın ITS (internal transcribed spacer) gen bölgesi ITS1 ve ITS4 evrensel primerleri kullanılarak çoğaltılmış ve dizilenmiştir. DNA nükleotid dizisi, NCBI GenBank veri tabanında BLAST işlemine sokulmuş ve veri tabanındaki diğer ilgili diziler ile karşılaştırılmıştır. Microsoft Office Word programı yardımı ile FASTA formatına çevrilen A.papavericola’nın DNA dizileri, MEGA 6.0 programı kullanılarak Tamura-Nei modellemesiyle Maximum Likelihood (ML) metodu kullanılarak filogenetik ağaç oluşturulmuştur.

Keywords

• Alternaria papavericola
• haşhaş
• moleküler karakterizasyon

Molecular Characterization of Alternaria papavericola, the Causal Agent of Crown and Capsule Blight of Poppy (Papaver somniferum)

Abstract

Both globally and in our country, the poppy (Papaver somniferum L.) is a significant medicinal and aromatic plant, with its seeds and oil serving as food. This work aimed to molecularly characterize Alternaria papavericola Woudenb. & Crous, the fungus that causes crown and capsule blight in poppies. The fungus was isolated from poppy seeds that were collected from the province of Isparta. For this purpose, the ITS (internal transcribed spacer) gene region of rDNA was amplified and sequenced using ITS1 and ITS4 universal primers. The DNA nucleotide sequence was BLASTed in the NCBI GenBank database and compared with other related sequences in the database. The DNA sequences of A.papavericola were converted to FASTA format with the help of the Microsoft Office Word program, and a phylogenetic tree was constructed using the Maximum Likelihood (ML) method with Tamura-Nei modeling using the MEGA 6.0 program.

Keywords

• Alternaria papavericola
• poppy
• molecular characterization

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