Cyclic 3-Hidroksimelatonin’in Tuz ve Su Stresi Altında Biber Tohumu Çimlenmesi Üzerine Etkileri

Abstract

Abiyotik stres koşulları tohum kalitesini bozarak, tohumun canlılığını ve çimlenme potansiyelini azaltmaktadır. Bu nedenle, abiyotik stres koşulları altında tohum çimlenmesini iyileştirmek önemlidir. Melatonin’in bitkilerde çeşitli abiyotik stres faktörlerine karşı tolerans kazanılmasında etkin rol aldığı ortaya konmuştur. Ancak, melatonin metabolitlerin bitkilerdeki olası rolleri üzerinde sınırlı sayıda çalışma yürütülmüş ve sonuçlar bu metabolitlerinde melatonin benzeri görevlere sahip olduğu yönündedir. O nedenle bu çalışmada bir melatonin metaboliti olan cyclic 3-hidroksimelatoninin (3-ohm) stres koşulları altında (kuraklık ve tuz stresi) tohum çimlenmesi üzerine etkileri araştırılmıştır. Bu amaca yönelik değişik konsantrasyonlarda (0 μM, 10 μM, 50 μM ve 100 μM) 3-ohm uygulanmış biber tohumları, tuz (0, 75, 100 ve 125 mM NaCl) ve su (0, 75 ve 100 gL⁻¹ PEG 6000) stresleri altında çimlenme testine tabi tutulmuştur. Çalışma sonucunda 3-ohm uygulamalarının tüm konsantrasyonlarının, tuz ve su stresi altındaki çimlenme testine tabi tutulan biber tohumlarının çimlenme yüzdelerini, çimlenme üniformitesini, çimlenme hızını, kökçük uzunluğu ve vigor indeksini kontrol uygulamasına kıyasla arttırdığı gözlenmiştir. Ayrıca 3-ohm’in biberde çimlenme aşamasında tuz ve su streslerine karşı toleransı arttırmada melatonin benzeri fonksiyona sahip olduğu ve antioksidan olarak görev yaparak stres toleransının artmasında aktif rol aldığı sonucunu ortaya çıkarmıştır.

Keywords

• Cyclic 3- Hidroksimelatonin
• tuzluluk stresi
• su stresi
• RNS
• ROS
• vigor indeksi

Project Number

Project number:121C409

Effects of Cyclic 3-hydroxymelatonin on Pepper Seed Germination Under Salt and Water Stress

Abstract

Abiotic stresses reduce seed germination by increasing deterioration of seed quality, reducing germination potential and seed viability. Therefore, it is important to improve seed germination under abiotic stress conditions. Melatonin has been shown to play an active role in the acquisition of tolerance to various abiotic stress factors in plants. However, limited studies have been conducted on the possible roles of melatonin metabolites in plants and the results suggests that these metabolites may also function similarly to melatonin. Therefore, in this study, the effects of cyclic 3-hydroxymelatonin (3-ohm), a melatonin metabolite, on seed germination under stress conditions (drought and salt stress) were investigated. For this purpose, pepper seeds treated with 3-ohm at different concentrations (0 μM, 10 μM, 50 μM and 100 μM) were subjected to germination test under salt (0, 75, 100 and 125 mM NaCl) and water (0, 75 and 100 gL⁻¹ PEG 6000) stresses. As a result of the study, all concentrations of 3-ohm treatments increased the germination percentage, germination uniformity, germination rate, root length and vigor index of pepper seeds subjected to germination test under salt and water stress compared to control treatment. It was also concluded that 3-ohm has a melatonin-like function in increasing tolerance to salt and water stresses at the germination stage in pepper and plays an active role in increasing stress tolerance by acting as an antioxidant.

Keywords

• Cyclic 3- Hydroxymelatonin
• Salinity stress
• Water stress
• RNS
• ROS
• vigor index

Supporting Institution

TUBITAK/BİDEB-2218

Project Number

Project number:121C409

Thanks

This study is a part of the project numbered 121C409 carried out with the support of TUBITAK/BİDEB-2218. I would like to thank TUBITAK for their support. I would also like to express my endless respect and gratitude to my esteemed teacher Assoc. Prof. Dr. Gökçen YAKUPOĞLU for his scientific support in my study.

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