Analysis of Genetic Diversity Among The Kiwifruit (Actinidia spp.) Genotypes Using Molecular Markers

Abstract

To reveal genetic diversity and provide information to conserve valuable kiwifruit genotypes, 42 kiwifruit genotypes collected from Ordu province of Northern Turkey were analyzed using a set of 11 sequence-related amplified polymorphism (SRAP) and 7 inter-primer binding site (iPBS) retrotransposons primers. Out of 85 amplified bands, a total of 40 polymorphic bands were obtained with SRAP primers. iPBS primers produced a total of 71 bands 24 of which were polymorphic. An average 5 and 3.42 polymorphic bands were obtained for SRAP and iPBS markers respectively. The similarity coefficients were calculated, and cluster analysis was performed with (NTSYSpc 2.2). Dendrograms were constructed via UPGMA based on SRAP and iPBS markers. The dendrograms constructed using the SRAP and iPBS data exhibited two clusters. The Mantel test for comparison of the SRAP and iPBS-based similarity matrices showed moderate but significant correlation (r=0.55). Most of the genotypes showed a low range of genetic diversity and more than half of the genotypes found genetically closely related with similarity coefficient ranged from 0.90 to 1.00. The result of current study showed that there is need to increase the genetic diversity of kiwifruit using different breeding approach such as mutations, crossing and germplasm introduction.

Keywords

• Kiwifruit
• genetic diversity
• SRAP
• iPBS
• molecular characterization

Moleküler İşaretleyiciler Kullanılarak Kivi (Actinidia spp.) Genotipleri Arasındaki Genetik Çeşitliliğin Analizi

Öz

Genetik çeşitliliği ortaya koymak ve değerli kivi genotiplerinin korunmasına yönelik bilgi sağlamak amacıyla, Kuzey Türkiye'nin Ordu ilinden toplanan 42 kivi genotipi, 11 dizi ilişkili çoğaltılmış polimorfizm (SRAP) ve 7 primerler arası bağlanma bölgesi (iPBS) retrotranspozon primerleri kullanılarak analiz edildi. 85 çoğaltılmış banttan, SRAP primerleri ile toplam 40 polimorfik bant elde edildi. iPBS primerleri, 24'ü polimorfik olan toplam 71 bant üretti. SRAP ve iPBS belirteçleri için sırasıyla ortalama 5 ve 3,42 polimorfik bant elde edildi. Benzerlik katsayıları hesaplandı ve kümeleme analizi (NTSYSpc 2.2) ile yapıldı. SRAP ve iPBS belirteçlerine dayalı UPGMA yoluyla dendrogramlar oluşturuldu. SRAP ve iPBS verileri kullanılarak oluşturulan dendrogramlar iki küme sergiledi. SRAP ve iPBS tabanlı benzerlik matrislerinin karşılaştırılması için yapılan Mantel testi, orta düzeyde ancak anlamlı bir korelasyon (r=0,55) göstermiştir. Genotiplerin çoğu düşük bir genetik çeşitlilik aralığına sahipken, genetik olarak yakın ilişkili bulunan genotiplerin yarısından fazlasının benzerlik katsayısı 0,90 ile 1,00 arasında değişmiştir. Mevcut çalışmanın sonuçları, mutasyon, çaprazlama ve germ plazması uygulaması gibi farklı ıslah yaklaşımları kullanılarak kivi genetik çeşitliliğinin artırılması gerektiğini göstermiştir.

Anahtar Kelimeler

• Kivi
• genetik çeşitlilik
• SRAP
• iPBS
• moleküler karakterizasyon

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