Vaccinium myrtillus ve Inula viscosa Bitki Ekstraktlarının Monilinia fructigena’ya Karşı Kontak Aktiveleri Üzerine Bir Değerlendirme

Öz

Bitki patojenlerinin kontrol edilmesinde kullanılan sentetik kimyasal ilaçların insan ve çevre sağlığı üzerine etkilerinin en alt düzeye indirilebilmesi için bitkisel ekstraktların kullanımı alternatif bir yöntem değerlendirilebilir. Bu çalışmayla; Vaccinium myrtillus (Yaban mersini) ve Inula viscosa (Yapışkan andız otu) bitkilerinin toprak üstü kısımlarından elde edilen methanol ekstraktının Monilinia fructigena (Honey in Whetze) üzerine antifungal aktivitesi in vitro koşullarda araştırılmıştır. Ekstraktların patojene karşı aktiviteleri agar petri yöntemi kullanılarak ile 1,33 mg/ml, 2 mg/ml ve 2,66 mg/ml dozların da belirlenmiştir. Ekstraktların patojenlerin miselyum gelişmeleri (MG) ve miselyum gelişim engellemeleri (MGE) hesaplanmıştır. Monilinia fructigena’nın miselyum gelişim engelleme üzerine I.viscosa ekstraktının 2,66 mg/ml dozda %74,42 V.myrtillus ekstraktının ise %57,92 oranında etkili olduğu belirlenmiştir. V.myrtillus ve I.viscosa bitki methanol ekstraktlarınında Monilinia fructigena’ya karşı in vitro koşullarda miselyum gelişimi üzerine etkili olduğu belirlenmiştir. Monilinia fructigena üzerine I.viscosa ekstraktının V.myrtillus ekstraktına göre daha etkili olduğu belirlenmiştir. Yürütülen çalışma, bitki patojenlerinin kontrolünde bitki ekstraktlarının kullanımının değerlenmesi için katkı sağlayacaktır.

Anahtar Kelimeler

• Antifungal etki
• bitkisel ekstrakt
• Vaccinium myrtillus
• Inula viscosa

An Evaluation on the Contact Activities of Vaccinium myrtillus and Inula viscosa Plant Extracts Against Monilinia fructigena

Abstract

The use of herbal extracts can be considered as an alternative method to minimize the effects of synthetic chemical pesticide used in the control of plant pathogens on human and environmental health. This study; Antifungal activity of methanol extract obtained from above ground parts of Vaccinium myrtillus (Blueberry) and Inula viscosa (False yellow head or Strong-Smelling Inula) plants on Monilinia fructigena (Honey in Whetze) was determined in vitro conditions. The pathogen activity of the extracts was determined by using agar petri method at doses of 1.33 mg/ml, 2 mg/ml and 2.66 mg/ml. Mycelial growth (MG) and mycelial growth inhibition (MGE) of pathogens of extracts were calculated. Monilinia fructigena on inhibition the development of mycelium; a dose 2.66 mg/ml dose, 74.42% of I.viscosa extract and 57.92% of I. myrtillus extract was determined to be effective. V.myrtillus and I.viscosa plant methanol extracts against Monilinia fructigena in vitro conditions were determined to be effective on the development of mycelium. Monilinia fructigena on I.viscosa extract was found to be more effective than V.myrtillus extract. The study, will contribute to the evaluation of the use of plant extracts in the control of plant pathogens.

Keywords

• Antifungal effect
• herbal extract
• Vaccinium myrtillus
• Inula viscosa

References

1. Al-Dissi, N.M., A.S. Salhab, H.A. Al-Hajj, 2001. Effects of Inula viscosa leaf extracts on abortion and implantation in rats. Journal of Ethnopharmacology 77:117-121.
2. Al-Masri, M.I., Harawi, S.M., Barakat, R.M., 2015. Effect of clammy Inula (Inula viscose) plant extract in combination with a low dose of the fungicide Iprodione on Botrytis cinerea in vitro and in vivo. American Journal of Plant Sciences 6:1519-1526.
3. Anonim, 2019. https://www.bizimbitkiler.org. tr/v2/hiyerarsi.php?c=inula (Erişim Tarihi: 01.11.2019).
4. Bayar, Y., A. Onaran, M. Yilar, F. Gul, 2018. Determination of the essential oil composition and the antifungal activities of bilberry (Vaccinium myrtillus L.) and bay laurel (Laurusnobilis L.). Journal of Essential Oil Bearing Plants 21(2):548-555.
5. Burt, S., 2004. Essential oils: their antibacterial properties and potential applications in foods a review. International Journal of Food Microbiology 94:223-253.
6. Cutler, H.G., R.A. Hill, B.G. Ward, B.H. Rohitha, A. Stewart, 1996. Antimicrobial, insecticidal and medicinal properties of natural products, flavors and fragrances. In: Takeoka, G.R., Teranishi R., Williams, P.J. and Kobayashi, A., Eds., Biotechnologies for Improved Foods and Flavours, American Chemical Society 51-66.
7. Giovanelli, G., S. Buratti, 2009. Comparison of polyphenolic composition and antioxidant activity of wild Italian blueberries and some cultivated varieties. Food Chemistry 112:903-908.
8. Hammer, K.A., Carson, C.F., Riley, T.V., 1999. Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology 86:985-990.

9. Jaakola, L., A. Tolvanen, K. Laine, A. Hohtola, 2001. Effect of N6-isopentenyladenine concentration on growth initiation in vitro and rooting of bilberry and lingonberry microshoots. Plant Cell Tiss Organ Cult. 66:73-77.
10. Kadıoğlu, İ., Y. Yanar, U. Asav, 2004. Allelopathic effects of plant extracts against seed germination of some weeds. Asian J. of Plant Sciences 3(4):472-475.
11. Kordali, S., A. Cakir, A. Mavi, H. Kilic, A. Yildirim, 2005. Screening of chemical composit ion and antifungal and antioxidant activities of the essential oils from three Turkish Artemisia species. Journal of Agricultural and Food Chemistry 53:1408-1416.
12. Lev, E., Z. Amar, 2000. Ethnopharmacological survey of traditional drugs sold in Israel at the end of the 20. Century. Journal of Ethnopharmacology 72:191-205.
13. Miljković, V., Nikolić, G., Mihajlov-Krstev, T.M., Arsić, B., 2018. Antibacterial activities of fruits extracts of three mulberry species (Morus alba L., Morus rubra L. and Morus nigra L.) and bilberry (Vaccinium myrtillus L.). Acta Medica Medianae 57(3):5-12.
14. Moze, S., Polak, T., Gasperlin, L., Koron, D., Vanzo, A., Ulrih, N.P., Abram, V., 2011. Phenolics in Slovenian bilberries (Vaccinium myrtillus L.) and blueberries (Vaccinium corymbosum L.). J. Agric. Food Chemistry 59(13):6998-7004.
15. Omezzine, F., M. Daami-Remadi, A. Rinez, A. Ladhari, R. Haouala, 2011. In vitro assessment of Inula spp. organic extracts for their antifungal activity against some pathogenic and antagonistic fungi. African Journal of Microbiology Research 5(21):3527-3531.
16. Pandey, D.K., N.N. Tripathi, R.D. Tripathi, S.N. Dixit, 1982. Fungitoxic and phytotoxic properties of essential oil of Hyptis suaandolens. Z. Pflanzenkrankheiten Pflanzenschutz 89:344-349.
17. Paster, N., B.J. Juven, E. Shaaya, M. Menassherov, R. Nitzan, H. Weisslowicz, U. Ravid, 1990. Inhibitory effect of oregano and thyme essential oils on moulds and food borne bacteria. Let Appl. Microbiol. 11:33-37.
18. Petróczy, M., Szigethy, A., Palkovics, L., 2012. Monilinia species in Hungary: morphology, culture characteristics, and molecular analysis. Trees: Structure and Function 26(1):153-164.
19. Pottier Alapetite, G., 1981. Flore de la Tunisie. Angiospermes-dicotylédones, Gamopétales. Programme flore et végétation tunisiennes, 655-1190.
20. Prabuseenivasan, S., Jayakumar, M., Ignacimuthu, S., 2006. In vitro antibacterial activity of some plant essential oils. BMC complementary Alternative Medicine, 6:39-45.
21. Wilkins, K.M., R.G. Board, 1989. Natural antimicrobial systems. In Mechanisms of Action of Food Preservation Procedures, 285-362.
22. Yılar, M., Y. Bayar, 2018. Antifungal activity of Thymbra spicata L. and Rosmarinus officinalis L. essential oils against Monilinia fructigena honey in whetze. Türk Tarım ve Doğa Bilimleri Dergisi 5(2):121-126.