Slovenya Fındıklarında Genetik Çeşitlilik

Abstract

Slovenya kendi Avrupa fındığı Corylus avellana L. genotiplerini ve yabani tiplerini bulunduran dünyadaki fındık üreticilerinden biridir. Slovenya fındık kaynaklarının moleküler genetik açısından gösterdiği çeşitlilik araştırılmalı ve bu fındık çeşitliliğinin potansiyelleri geliştirilmelidir. Bu amaçla, Slovenya’nın geniş coğrafik alanını temsil eden 57 Slovenya fındık tipi ve Slovenya dünya koleksiyonundan 45 fındık çeşidi, 11 seçici AFLP primer çifti ve 50 SSR primer çifti ile taranmıştır. Toplamda, 512 polimorfik SSR fragmenti ve 532 polimorfik AFLP fragmenti analiz edilmiştir. Slovenya fındık çeşitleri ve tipleri, AFLP verisinin hoc istatistiği ve Neigbor-joining algoritması (r=0.96) ile analizi sonucunda üç kümeye ayrılmıştır. Benzer olarak, SSR verisi (r=0.96) Neigbor-joining algoritması ile üç kümeye ayrılmıştır. SSR verileriyle 28 fındık tipi, AFLP verileriyle 33 fındık tipi Slovenya fındık çekirdek koleksiyonu için seçilmiştir. Seçilen çekirdek koleksiyon, en yüksek genetik çeşitliliği en az birey ile temsil etmektedir ve seçilen fındıklardan 18 tanesi her iki çekirdek koleksiyonunda ortak olarak bulunmaktadır. Slovenya fındık çeşitliliğinin çalışılması, ilgilenilen agronomik ve biyokimyasal özelliklerin genetik haritalanması ve tanımlanması için ilk adımdır.

Keywords

• AFLP Çoğaltılmış fragment uzunluk polimorfizmi
• SSR basit dizi tekrarları
• moleküler markörler

Genetic Diversity in Slovenian Hazelnuts

Abstract

Slovenia is the one of the hazelnut producers in the world and has its own European hazelnut (Corylus avellana L.) genotypes and wild types. Slovenian hazelnut resources should be examined for their molecular genetic diversity and their potential for improvement of hazelnut. For this reason, 102 Slovenian hazelnuts representing a wide geographic range of the country were screened with 11 pairs of selective AFLP primers and 50 pairs of SSR primers. In total, 512 polymorphic SSR fragments and 532 polymorphic AFLP fragments were found. Diversity analysis of the Slovenian hazelnut genotypes indicated that they were classified to three clusters according to hoc statistics and Neighbor-joining algorithms (r= 0.96) for AFLP data. Similarly, three clusters were observed for the SSR data using the Neighbor joining algorithm (r= 0.96). A total of 28 individuals with SSR data and 33 individuals with AFLP data were selected for core collections of Slovenian hazelnut genotypes. These core collections represent a maximum of genetic diversity in a minimum number of individuals and 18 of them were present in both core collection datasets. Examination of Slovenian hazelnut diversity is a first step toward identification and genetic mapping of agronomic and biochemical traits of interest.

Keywords

• AFLP amplified fragment length polymorphism
• SSR simple sequence repeats
• molecular marker

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