Construction of the Reference Genetic Linkage Map of Amasya Apple by Microsatellite (SSR) Markers

Abstract

This study aimed to construct reference genetic map of ‘Amasya’ apple cultivar with SSR markers. With this aimed, the cross between selected ‘Kaşel-41’ from ‘Amasya’ apple cultivar and ‘Willams Pride’ cultivar establishes this study's materials. As a result of screening 699 SSR pairs of primer in 96 hybrid individuals showed 417 polymorphisms. A reference map was constructed by using 207 common SSR markers. The reference genetic map was 1351 cM long , average marker number 12.17 with a mean marker density of 6.52. Thereby producing the densest SSR-based reference map currently reported for apple. In the future genetic mapping and genomic studies, use of the new SSR markers and other marker methods will be useful to obtain high density map.

Keywords

• Apple
• SSR
• reference map
• microsatellite

SSR Markörleri İle Amasya Elmasının İskelet Haritasının Oluşturulması

Abstract

Bu çalışmada ‘Amasya’ elmasının SSR markörleri ile iskelet haritasının oluşturulması amaçlanmıştır. Bu amaçla Amasya elması içerisinden seçilen ‘Kaşel-41’ çeşidi ile ‘Williams Pride’ çeşitlerinin melezlenmesinden oluşan F1 popülasyonu çalışmanın materyalini oluşturmuştur. SSR primer çiftlerinin taranması sonucunda 699 primer çiftinden 417 adedi açılım göstermiş ve bunlardan 207 adet ortak SSR markörü olarak 96 melez bireyde analiz edilerek iskelet harita oluşturulmuştur. Amasya elmasının iskelet haritasının oluşturulmasında açılım gösteren 207 markör, 17 bağlantı grubuna dağılım göstermiştir. Bu harita 1351 cM uzunluğunda olup, markör yoğunluğu 12.2 ve markörler arası ortalama uzaklık ise 6.5 cM olarak saptanmıştır. Böylece elmada şimdiye kadarki en iyi SSR markörleri ile iskelet harita bu çalışma ile oluşturulmuştur. Yeni SSR markörleri ve farklı markör yöntemlerinin kullanılması, ileriki zamanlarda yapılacak genetik haritalama çalışmalarında yüksek yoğunluklu harita elde edilmesinde fayda sağlayacaktır.

Keywords

• Elma
• SSR
• iskelet harita
• mikrosatellit

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